{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T17:31:17Z","timestamp":1793381477176,"version":"4.5.0"},"reference-count":0,"publisher":"SNF Swedish Nutrition Foundation","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["fnr"],"abstract":"<jats:p>Background: Neuroinflammation is a key pathological feature in many neurodegenerative diseases, and the nuclear factor kappa-B (NF-\u03baB) signaling pathway is a central mediator of this response. Aged garlic extract (AGE) is a functional food with well-documented antioxidant and anti-inflammatory properties, but its role in mitigating neuroinflammation remains unclear.\r\nObjective: This study investigates the effects of AGE on neuroinflammation by modulating the NF-\u03baB signaling pathway using multi-omics analyses and experimental validation.\r\nDesign: Lipopolysaccharide (LPS)-induced BV2 microglial cells and LPS-treated C57BL\/6 mice were used to assess the effects of AGE. Transcriptomics, metabolomics, and network pharmacology approaches identified potential targets and pathways, focusing on NF-\u03baB signaling. In vitro and in vivo models were employed to evaluate behavioral, biochemical, and histological outcomes.\r\nResults: AGE reduced pro-inflammatory cytokines (tumor necrosis factor-\u03b1, interleukin-1\u03b2, inducible nitric oxide synthase, and cyclooxygenase-2) in LPS-stimulated BV2 cells and suppressed microglial activation and neuronal damage in LPS-induced mice. Transcriptomic analysis showed that NF-\u03baB pathway inhibition mediated these effects, with molecular docking confirming interactions between aged garlic compounds and NF-\u03baB targets (NF-\u03baB2 and NF-\u03baB3).\r\nConclusion: AGE attenuates neuroinflammation by inhibiting the NF-\u03baB signaling pathway, improving cognitive and motor functions, and reducing neuronal injury in experimental models. These findings suggest aged garlic as a promising neuroprotective agent against neuroinflammation.<\/jats:p>","DOI":"10.29219\/fnr.v69.11923","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T21:11:36Z","timestamp":1752095496000},"source":"Crossref","is-referenced-by-count":4,"title":["Aged garlic attenuates neuroinflammation via modulating the NF-\u03baB pathway: Insights from multi-omics analyses"],"prefix":"10.29219","author":[{"given":"Junjun","family":"Meng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengquan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofan","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxiu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyuan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxue","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxiu","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"11752","published-online":{"date-parts":[[2025,7,9]]},"container-title":["Food &amp; Nutrition Research"],"original-title":[],"link":[{"URL":"https:\/\/foodandnutritionresearch.net\/index.php\/fnr\/article\/download\/11923\/19678","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foodandnutritionresearch.net\/index.php\/fnr\/article\/download\/11923\/19675","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foodandnutritionresearch.net\/index.php\/fnr\/article\/download\/11923\/19676","content-type":"application\/epub+zip","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foodandnutritionresearch.net\/index.php\/fnr\/article\/download\/11923\/19677","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/foodandnutritionresearch.net\/index.php\/fnr\/article\/download\/11923\/19678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T21:11:37Z","timestamp":1752095497000},"score":1,"resource":{"primary":{"URL":"https:\/\/foodandnutritionresearch.net\/index.php\/fnr\/article\/view\/11923"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,9]]},"references-count":0,"URL":"https:\/\/doi.org\/10.29219\/fnr.v69.11923","relation":{},"ISSN":["1654-661X","1654-6628"],"issn-type":[{"value":"1654-661X","type":"electronic"},{"value":"1654-6628","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7,9]]}}}