{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T19:42:00Z","timestamp":1696448520221},"reference-count":0,"publisher":"Colegio Mexicano de Inmunologia Clinica y Alergia, A. C.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["RAM"],"abstract":"<jats:p>Antecedentes: las opciones diagn\u00f3sticas de las reacciones inmunol\u00f3gicas a la picadura del mosquito son limitadas. En Cuba, las reacciones mediadas por IgE m\u00e1s frecuentes son por picadura de Culex quinquefasciatus. Objetivo: determinar la sensibilidad y especificidad de la prueba cut\u00e1nea por punci\u00f3n con dos dosis del extracto estandarizado en unidades de nitr\u00f3geno proteico (UNP) de Culex quinquefasciatus (BIOCEN, Cuba). Material y m\u00e9todo: estudio anal\u00edtico efectuado en 100 ni\u00f1os entre 2 y 15 a\u00f1os de edad: 50 pacientes at\u00f3picos con antecedentes de alergia a la picadura de mosquito e IgE s\u00e9rica espec\u00edfica positiva a Culex quinquefasciatus y 50 pacientes at\u00f3picos sin antecedentes de alergia a la picadura de mosquito e IgE s\u00e9rica espec\u00edfica negativa a Culex quinquefasciatus. La prueba cut\u00e1nea por punci\u00f3n se realiz\u00f3 por duplicado en los antebrazos de los pacientes. Las dosis investigadas fueron 100 y 10 UNP\/mL. Resultados: en la prueba cut\u00e1nea por punci\u00f3n con el extracto de mayor concentraci\u00f3n se obtuvo un tama\u00f1o del \u00e1rea del hab\u00f3n de 22.09 mm2 y con la menor concentraci\u00f3n de 8.19 mm2; una diferencia estad\u00edsticamente significativa (p=0.001, prueba t de Student). La prueba cut\u00e1nea positiva se correlacion\u00f3 en el 100% de los pacientes con la existencia de IgE espec\u00edfica. La prueba con ambas dosis mostr\u00f3 94% de especificidad y 88% de sensibilidad. Conclusi\u00f3n: la alta coincidencia en el resultado de la prueba cut\u00e1nea nos muestra que puede sustituirse la concentraci\u00f3n del extracto a 100 UNP\/mL por la de menor concentraci\u00f3n, sin perder confiabilidad en el diagn\u00f3stico de sensibilizaci\u00f3n al mosquito Culex quinquefasciatus, utilizando ese m\u00e9todo in vivo.<\/jats:p>","DOI":"10.29262\/ram.v63i1.114","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T20:59:48Z","timestamp":1515531588000},"page":"11-19","source":"Crossref","is-referenced-by-count":2,"title":["Sensibilidad y especificidad de la prueba cut\u00e1nea por punci\u00f3n con dos concentraciones del extracto estandarizado de Culex quinquefasciatus en ni\u00f1os al\u00e9rgicos"],"prefix":"10.29262","volume":"63","author":[{"given":"Ra\u00fal L\u00e1zaro","family":"Castro-Almarales","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirta","family":"\u00c1lvarez-Castell\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercedes","family":"Ronquillo-D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 S","family":"Rodr\u00edguez-Canosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayda","family":"Gonz\u00e1lez-Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara I","family":"Navarro-Viltre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Betancourt-Mesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Enr\u00edquez-Dom\u00ednguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Carmen","family":"Reyes-Zamora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunia","family":"Oliva-D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayte\u00e9","family":"Mateo-Morej\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis","family":"Labrada-Rosado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"11808","published-online":{"date-parts":[[2016,3,1]]},"container-title":["Revista Alergia M\u00e9xico"],"original-title":[],"link":[{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/114\/226","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/114\/288","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/viewFile\/114\/250","content-type":"application\/epub+zip","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/114\/250","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/114\/226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T22:05:51Z","timestamp":1627682751000},"score":1,"resource":{"primary":{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/view\/114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,1]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,3,1]]}},"URL":"https:\/\/doi.org\/10.29262\/ram.v63i1.114","relation":{},"ISSN":["2448-9190","0002-5151"],"issn-type":[{"value":"2448-9190","type":"electronic"},{"value":"0002-5151","type":"print"}],"subject":[],"published":{"date-parts":[[2016,3,1]]}}}