{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T12:32:52Z","timestamp":1649075572878},"reference-count":0,"publisher":"Colegio Mexicano de Inmunologia Clinica y Alergia, A. C.","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["RAM"],"abstract":"<jats:p>ANTECEDENTES: algunos adenovirus se han se\u00f1alado como activadores clonales en leucemias. El alelo HLA-DRB1* 14 subtipos DRB1*14:21, 14:22, 14:45, 14:26, 14:33, 14:51, 14:35 se asociaron con leucemia mieloide cr\u00f3nica (LMC) en pacientes venezolanos.OBJETIVO: evaluar el mimetismo molecular entre el adenovirus y la estructura del ant\u00edgeno HLA-DRB1*14 que exhiben el mismo cambio en la posici\u00f3n de amino\u00e1cido del ep\u00edtopo DR53.MATERIAL Y M\u00c9TODO: estudio experimental realizado en el IHO Banco de Sangre del Estado Zulia, Venezuela en muestras de sangre perif\u00e9rica de pacientes con LLA, LMC y controles sanos. Se realizaron cultivo mixto de linfocitos, serolog\u00eda, proliferaci\u00f3n linfocitaria y citofluorometr\u00eda.RESULTADOS: los linfocitos DRB1*14 del paciente reaccionaron en 48 horas versus los linfocitos DRB1*14 estimuladores, que exhibieron aumento de los linfocitos T CD8+. Los pacientes con LMC tuvieron un perfil serol\u00f3gico diferente contra el adenovirus. S\u00f3lo pacientes con LMC reaccionaron frente al p\u00e9ptido secuencia LLERRRA con incremento de las c\u00e9lulas TCD8+.CONCLUSI\u00d3N: se estableci\u00f3 que la relaci\u00f3n leucemia mieloide cr\u00f3nica, HLA-DRB1*14, c\u00e9lulas TCD8+ de memoria autorreactivas y TCD8+ en respuesta espec\u00edfica frente al adenovirus podr\u00eda estar en el origen de la leucemia mieloide cr\u00f3nica de pacientes venezolanos.<\/jats:p>","DOI":"10.29262\/ram.v63i2.149","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T21:00:46Z","timestamp":1515531646000},"page":"163-168","source":"Crossref","is-referenced-by-count":0,"title":["Linfocitos TCD8+ autorreactivos en pacientes con leucemia mieloide cr\u00f3nica en asociaci\u00f3n con HLA e infecci\u00f3n con adenovirus"],"prefix":"10.29262","volume":"63","author":[{"given":"Sergio E.","family":"Rivera-Pirela","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Echeverr\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Salcedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgina","family":"M\u00e1rquez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuhey","family":"Carrillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yennis","family":"Parra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Mar\u00eda","family":"Cipriani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 R.","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melchor","family":"\u00c1lvarez de Mon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atilio","family":"Farruco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"11808","published-online":{"date-parts":[[2016,5,11]]},"container-title":["Revista Alergia M\u00e9xico"],"original-title":[],"link":[{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/149\/268","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/149\/305","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/viewFile\/149\/280","content-type":"application\/epub+zip","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/149\/280","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/download\/149\/268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T22:00:13Z","timestamp":1627682413000},"score":1,"resource":{"primary":{"URL":"http:\/\/revistaalergia.mx\/ojs\/index.php\/ram\/article\/view\/149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,11]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,5,11]]}},"URL":"https:\/\/doi.org\/10.29262\/ram.v63i2.149","relation":{},"ISSN":["2448-9190","0002-5151"],"issn-type":[{"value":"2448-9190","type":"electronic"},{"value":"0002-5151","type":"print"}],"subject":[],"published":{"date-parts":[[2016,5,11]]}}}