{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:30:49Z","timestamp":1705537849734},"reference-count":14,"publisher":"Colegio Mexicano de Inmunologia Clinica y Alergia, A. C.","issue":"4","license":[{"start":{"date-parts":[[2023,12,31]],"date-time":"2023-12-31T00:00:00Z","timestamp":1703980800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Alerg Mex"],"abstract":"<jats:p>Despu\u00e9s del descubrimiento de la IgE, los avances tecnol\u00f3gicos han proporcionado nuevas herramientas de\u00a0laboratorio para la cuantificaci\u00f3n de anticuerpos IgE espec\u00edficos de al\u00e9rgenos en suero y en la superficie de\u00a0bas\u00f3filos-mastocitos. Las pruebas in vitro ofrecen numerosas ventajas: cuantificaci\u00f3n precisa, falta de interferencia\u00a0de f\u00e1rmacos, seguridad y almacenamiento a largo plazo de las muestras. Los inmunoensayos cuantitativos\u00a0para anticuerpos IgE pueden ser un complemento de las pruebas cut\u00e1neas. El reactivo de alergeno\u00a0en fase s\u00f3lida (alergosorbente) o l\u00edquida es el componente principal del ensayo que confiere especificidad a la\u00a0prueba de anticuerpos IgE. Es el reactivo m\u00e1s complejo y altamente variable en los ensayos de anticuerpos IgE.\u00a0La elecci\u00f3n de utilizar recombinantes de diagn\u00f3stico en una \u00fanica plataforma en lugar de m\u00faltiples se realiza\u00a0caso por caso (considerando el historial previo y el perfil cl\u00ednico) y de manera dependiente de los al\u00e9rgenos.\u00a0Aunque la mayor parte de las alergias alimentarias se limitan a una peque\u00f1a cantidad de posibles desencadenantes,\u00a0estos alimentos son muy complejos al momento de evaluar su potencial alerg\u00e9nico. La posibilidad de\u00a0fraccionar el al\u00e9rgeno y entender algunos de sus componentes como potencialmente importantes para definir\u00a0el riesgo de reacci\u00f3n cl\u00ednica, reactividad cruzada o persistencia de la alergia, abri\u00f3 una nueva era en el campo\u00a0de la alergia, denominada alergia molecular. La identificaci\u00f3n del componente alerg\u00e9nico responsable de las\u00a0reacciones supone una herramienta importante para confirmar la informaci\u00f3n y gravedad de los s\u00edntomas,\u00a0historia natural de la enfermedad, posibilidad de reactividad cruzada y cl\u00ednica (marcadores de alergia).\u00a0<\/jats:p>","DOI":"10.29262\/ram.v70i4.1335","type":"journal-article","created":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T12:36:33Z","timestamp":1705494993000},"page":"245-249","source":"Crossref","is-referenced-by-count":0,"title":["RAST, Inmunoblot, Inmunocap e ISAC en alergia alimentaria"],"prefix":"10.29262","volume":"70","author":[{"given":"Herberto J.","family":"Chong Neto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"11808","published-online":{"date-parts":[[2023,12,31]]},"reference":[{"key":"56547","doi-asserted-by":"crossref","unstructured":"Yunginger JW, Ashlstedt S, Ownby DR, et al. 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