{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T16:27:31Z","timestamp":1748363251107,"version":"3.40.5"},"reference-count":38,"publisher":"American Mosquito Control Association","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n        <jats:p>We report the results of a laboratory sensitivity and specificity evaluation of the Rapid Analyte Measurement Platform (RAMP\u00ae) Dengue Virus (DENV) antigen detection assay, which is designed to detect all serotypes of DENV in mosquito pools. The RAMP DENV assay was able to detect geographically distinct strains of all 4 DENV serotypes in virus-spiked mosquito pools that contained at least 4.3 log<jats:sub>10<\/jats:sub> plaque forming units\/ml, although discrete sensitivity limits varied slightly for each serotype. The RAMP DENV assay also detected DENV 1\u20134 in mosquito pools containing a single infected mosquito and 24 laboratory-reared uninfected mosquitoes. No false positives were detected in negative control mosquito pools or in samples containing high titers of nontarget arboviruses. We found that while the kit-supplied RAMP buffer reduced the infectious titer of DENV, it did not completely inactivate all serotypes. We recommend adding a detergent, Triton X-100, to the buffer to ensure complete inactivation of DENV if the assay is to be conducted at a lower biosafety level than required for DENV handling.<\/jats:p>","DOI":"10.2987\/21-7013.1","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T17:33:40Z","timestamp":1629308020000},"page":"152-156","source":"Crossref","is-referenced-by-count":1,"title":["Laboratory Evaluation of the Rapid Analyte Measurement Platform Assay to Detect Dengue Virus in Mosquito Pools"],"prefix":"10.2987","volume":"37","author":[{"given":"Kristen L.","family":"Burkhalter","sequence":"first","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, 3156 Rampart Road, Fort Collins, CO 80521"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry M.","family":"Savage","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, 3156 Rampart Road, Fort Collins, CO 80521"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1569","reference":[{"key":"i8756-971X-37-3-152-Adams1","series-title":"Am J Trop Med Hyg","first-page":"884","article-title":"Epidemiology of dengue, chikungunya, and Zika virus disease in U.S. states and territories, 2017","volume":"101","author":"Adams","year":"2019","unstructured":"Adams\n              LE,\n            \n            \n              Martin\n              SW,\n            \n            \n              Lindsey\n              NP,\n            \n            \n              Lehman\n              JA,\n            \n            \n              Rivera\n              A,\n            \n            \n              Kolsin\n              J,\n            \n            \n              Landry\n              K,\n            \n            \n              Staples\n              JE,\n            \n            \n              Sharp\n              TM,\n            \n 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present and future prospects","volume":"5","author":"Murray","year":"2013","unstructured":"Murray\n              NE,\n            \n            \n              Quam\n              MB,\n            \n            \n              Wilder-Smith\n              A.\n            \n          \n          2013.\n\t\t\t\t\tEpidemiology of dengue: past, present and future prospects.\n\t\t\t\t\tClin Epidemiol5:\n\t\t\t\t\t299\u2013\n\t\t\t\t\t309."},{"key":"i8756-971X-37-3-152-Radke1","series-title":"Emer Infect Dis","first-page":"135","article-title":"Dengue outbreak in Key West, Florida, USA, 2009","volume":"18","author":"Radke","year":"2012","unstructured":"Radke\n              EG,\n            \n            \n              Gregory\n              CJ,\n            \n            \n              Kintziger\n              KW,\n            \n            \n              Sauber-Schatz\n              EK,\n            \n            \n              Hunsperger\n              EA,\n            \n            \n  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