{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:42:45Z","timestamp":1761396165047,"version":"3.40.5"},"reference-count":30,"publisher":"American Mosquito Control Association","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n        <jats:p>Although the specific cDNA amplification mechanisms of reverse-transcriptase polymerase chain reaction (RT-PCR) and RT loop-mediated isothermal amplification (RT-LAMP) are very different, both molecular assays serve as options to detect arboviral RNA in mosquito pools. Like RT-PCR, RT-LAMP uses a reverse transcription step to synthesize complementary DNA (cDNA) from an RNA template and then uses target-specific primers to amplify cDNA to detectable levels in a single-tube reaction. Using laboratory-generated West Nile virus (WNV) samples and field-collected mosquito pools, we evaluated the sensitivity and specificity of a commercially available WNV real-time RT-LAMP assay (Pro-AmpRT\u2122 WNV; Pro-Lab Diagnostics, Inc., Round Rock, Texas) and compared the results to a validated real-time RT-PCR assay. Laboratory generated virus stock samples containing \u2265 2.3 log<jats:sub>10<\/jats:sub> plaque-forming units (PFU)\/ml and intrathoracically inoculated mosquitoes containing \u2265 2.4 log<jats:sub>10<\/jats:sub> PFU\/ml produced positive results in the Pro-AmpRT WNV assay. Of field-collected pools that were WNV positive by real-time RT-PCR, 74.5% (70 of 94) were also positive by the Pro-AmpRT WNV assay, resulting in an overall Cohen's kappa agreement of 79.4% between the 2 tests. The Pro-AmpRT WNV assay shows promise as a suitable virus screening tool for vector surveillance programs provided agencies are aware of its characteristics and limitations.<\/jats:p>","DOI":"10.2987\/21-7033","type":"journal-article","created":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:24:26Z","timestamp":1637713466000},"page":"256-262","source":"Crossref","is-referenced-by-count":2,"title":["Laboratory and Field Evaluations of a Commercially Available Real-Time Loop-Mediated Isothermal Amplification Assay for the Detection of West Nile Virus in Mosquito Pools"],"prefix":"10.2987","volume":"37","author":[{"given":"Kristen L.","family":"Burkhalter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"O'Keefe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary","family":"Holbert-Watson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodore","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry M.","family":"Savage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel M.","family":"Markowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1569","reference":[{"key":"i8756-971X-37-4-256-Beaty1","series-title":"Arbovirology. 7th edition","first-page":"189","article-title":"Diagnostic procedures for viral, rickettsial and chlamydial infections","author":"Beaty","year":"1995","unstructured":"Beaty\n              BJ,\n            \n            \n              Calisher\n              CH,\n            \n            \n              Shope\n              RS.\n            \n          \n          1995.\n\t\t\t\t\tDiagnostic procedures for viral, rickettsial and chlamydial infections.\n\t\t\t\t\tIn:LenneteET,LenneteDA,eds.Arbovirology. 7th edition.\n\t\t\t\t\tWashington, DC:\n\t\t\t\t\tAmerican Public Health Association.\n\t\t\t\t\tp189\u2013\n\t\t\t\t\t212."},{"key":"i8756-971X-37-4-256-Burkhalter1","series-title":"J Am Mosq Control Assoc","first-page":"21","article-title":"Evaluation of a rapid analyte measurement platform and real-time reverse-transcriptase polymerase chain reaction assay West Nile virus detection system in mosquito pools","volume":"30","author":"Burkhalter","year":"2014","unstructured":"Burkhalter\n              KL,\n            \n            \n              Horiuchi\n              K,\n            \n            \n              Biggerstaff\n              BJ,\n            \n            \n              Savage\n              HM,\n            \n            \n              Nasci\n              RS.\n            \n          \n          2014.\n\t\t\t\t\tEvaluation of a rapid analyte measurement platform and real-time reverse-transcriptase polymerase chain reaction assay West Nile virus detection system in mosquito pools.\n\t\t\t\t\tJ Am Mosq Control Assoc30:\n\t\t\t\t\t21\u2013\n\t\t\t\t\t30."},{"key":"i8756-971X-37-4-256-Burkhalter2","series-title":"J Am Mosq Control Assoc","first-page":"64","article-title":"Evaluation of commercial assays for detecting West Nile Virus antigen","volume":"22","author":"Burkhalter","year":"2006","unstructured":"Burkhalter\n              KL,\n            \n            \n              Lindsay\n              R,\n            \n            \n              Anderson\n              R,\n            \n            \n              Dibernardo\n              A,\n            \n            \n              Fong\n              W,\n            \n            \n              Nasci\n              RS.\n            \n          \n          2006.\n\t\t\t\t\tEvaluation of commercial assays for detecting West Nile Virus antigen.\n\t\t\t\t\tJ Am Mosq Control Assoc22:\n\t\t\t\t\t64\u2013\n\t\t\t\t\t69."},{"key":"i8756-971X-37-4-256-Chung1","series-title":"JAMA","first-page":"297","article-title":"The 2012 West Nile encephalitis epidemic in Dallas, Texas","volume":"310","author":"Chung","year":"2013","unstructured":"Chung\n              WM,\n            \n            \n              Buseman\n              CM,\n            \n            \n              Joyner\n              SN,\n            \n            \n              Hughes\n              SM,\n            \n            \n              Fomby\n              TB,\n            \n            \n              Luby\n              JP,\n            \n            \n              Haley\n              RW.\n            \n          \n          2013.\n\t\t\t\t\tThe 2012 West Nile encephalitis epidemic in Dallas, Texas.\n\t\t\t\t\tJAMA310:\n\t\t\t\t\t297\u2013\n\t\t\t\t\t307."},{"key":"i8756-971X-37-4-256-daSilva1","series-title":"Sci Rep","first-page":"4494","article-title":"Development and validation of reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in mosquito samples from Brazil","volume":"9","author":"da Silva","year":"2019","unstructured":"da Silva\n              SJR,\n            \n            \n              Paiva\n              MHS,\n            \n            \n              Guedes\n              DRD,\n            \n            \n              Krokovsky\n              L,\n            \n            \n              Melo\n              FL,\n            \n            \n              da Silva\n              MAL,\n            \n            \n              da Silva\n              A,\n            \n            \n              Ayres\n              CFJ,\n            \n            \n              Pena\n              LJ.\n            \n          \n          2019.\n\t\t\t\t\tDevelopment and validation of reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in mosquito samples from Brazil.\n\t\t\t\t\tSci Rep9:\n\t\t\t\t\t4494."},{"key":"i8756-971X-37-4-256-DeFelice1","series-title":"JAMA Netw Open","first-page":"e193175","article-title":"Modeling and surveillance of reporting delays of mosquitoes and humans infected with West Nile virus and associations with accuracy of West Nile virus forecasts","volume":"2","author":"DeFelice","year":"2019","unstructured":"DeFelice\n              NB,\n            \n            \n              Birger\n              R,\n            \n            \n              DeFelice\n              N,\n            \n            \n              Gagner\n              A,\n            \n            \n              Campbell\n              SR,\n            \n            \n              Romano\n              C,\n            \n            \n              Santoriello\n              M,\n            \n            \n              Henke\n              J,\n            \n            \n              Wittie\n              J,\n            \n            \n              Cole\n              B,\n            \n            \n              Kaiser\n              C,\n            \n            \n              Shaman\n              J.\n            \n          \n          2019.\n\t\t\t\t\tModeling and surveillance of reporting delays of mosquitoes and humans infected with West Nile virus and associations with accuracy of West Nile virus forecasts.\n\t\t\t\t\tJAMA Netw Open2:\n\t\t\t\t\te193175."},{"key":"i8756-971X-37-4-256-DeFelice2","series-title":"Nat Commun","first-page":"14592","article-title":"Ensemble forecast of human West Nile virus cases and mosquito infection rates","volume":"8","author":"DeFelice","year":"2017","unstructured":"DeFelice\n              NB,\n            \n            \n              Little\n              E,\n            \n            \n              Campbell\n              SR,\n            \n            \n              Shaman\n              J.\n            \n          \n          2017.\n\t\t\t\t\tEnsemble forecast of human West Nile virus cases and mosquito infection rates.\n\t\t\t\t\tNat Commun8:\n\t\t\t\t\t14592."},{"key":"i8756-971X-37-4-256-Francois1","series-title":"FEMS Immunol Med Microbiol","first-page":"41","article-title":"Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications","volume":"62","author":"Francois","year":"2011","unstructured":"Francois\n              P,\n            \n            \n              Tangomo\n              M,\n            \n            \n              Hibbs\n              J,\n            \n            \n              Bonetti\n              EJ,\n            \n            \n              Boehme\n              CC,\n            \n            \n              Notomi\n              T,\n            \n            \n              Perkins\n              MD,\n            \n            \n              Schrenzel\n              J.\n            \n          \n          2011.\n\t\t\t\t\tRobustness of a loop-mediated isothermal amplification reaction for diagnostic applications.\n\t\t\t\t\tFEMS Immunol Med Microbiol62:\n\t\t\t\t\t41\u2013\n\t\t\t\t\t48."},{"key":"i8756-971X-37-4-256-Karki1","series-title":"PLoS One","first-page":"e0227160","article-title":"The drivers of West Nile virus human illness in the Chicago, Illinois, USA area: fine scale dynamic effects of weather, mosquito infection, social, and biological conditions","volume":"15","author":"Karki","year":"2020","unstructured":"Karki\n              S,\n            \n            \n              Brown\n              WM,\n            \n            \n              Uelmen\n              J,\n            \n            \n              Ruiz\n              MO,\n            \n            \n              Smith\n              RL.\n            \n          \n          2020.\n\t\t\t\t\tThe drivers of West Nile virus human illness in the Chicago, Illinois, USA area: fine scale dynamic effects of weather, mosquito infection, social, and biological conditions.\n\t\t\t\t\tPLoS One15:\n\t\t\t\t\te0227160."},{"key":"i8756-971X-37-4-256-Kilpatrick1","series-title":"Am J Epidemiol","first-page":"829","article-title":"Predicting human West Nile virus infections with mosquito surveillance data","volume":"178","author":"Kilpatrick","year":"2013","unstructured":"Kilpatrick\n              AM,\n            \n            \n              Pape\n              JW.\n            \n          \n          2013.\n\t\t\t\t\tPredicting human West Nile virus infections with mosquito surveillance data.\n\t\t\t\t\tAm J Epidemiol178:\n\t\t\t\t\t829\u2013\n\t\t\t\t\t835."},{"key":"i8756-971X-37-4-256-Kwan1","series-title":"Emerg Infect Dis","first-page":"1298","article-title":"Comparison of enzootic risk measures for predicting West Nile disease, Los Angeles, California, USA, 2004\u20132010","volume":"8","author":"Kwan","year":"2012","unstructured":"Kwan\n              JL,\n            \n            \n              Park\n              BK,\n            \n            \n              Carpenter\n              TE,\n            \n            \n              Ngo\n              V,\n            \n            \n              Civen\n              R,\n            \n            \n              Reisen\n              WK.\n            \n          \n          2012.\n\t\t\t\t\tComparison of enzootic risk measures for predicting West Nile disease, Los Angeles, California, USA, 2004\u20132010.\n\t\t\t\t\tEmerg Infect Dis8:\n\t\t\t\t\t1298\u2013\n\t\t\t\t\t1306."},{"key":"i8756-971X-37-4-256-Lanciotti1","series-title":"J Clin Microbiol","first-page":"4066","article-title":"Rapid detection of West Nile virus from human clinical specimens, field collected mosquitoes, and avian samples by a TaqMan RT-PCR assay","volume":"38","author":"Lanciotti","year":"2000","unstructured":"Lanciotti\n              RS,\n            \n            \n              Kerst\n              AJ,\n            \n            \n              Nasci\n              RS,\n            \n            \n              Godsey\n              MS,\n            \n            \n              Mitchell\n              CJ,\n            \n            \n              Savage\n              HM,\n            \n            \n              Komar\n              N,\n            \n            \n              Panella\n              NA.\n            \n          \n          2000.\n\t\t\t\t\tRapid detection of West Nile virus from human clinical specimens, field collected mosquitoes, and avian samples by a TaqMan RT-PCR assay.\n\t\t\t\t\tJ Clin Microbiol38:\n\t\t\t\t\t4066\u2013\n\t\t\t\t\t4071."},{"key":"i8756-971X-37-4-256-LeRoux1","series-title":"J Clin Microbiol","first-page":"645","article-title":"Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid detection of Rift Valley fever virus in clinical specimens","volume":"47","author":"Le Roux","year":"2009","unstructured":"Le Roux\n              CA,\n            \n            \n              Kubo\n              T,\n            \n            \n              Grobbelaar\n              AA,\n            \n            \n              van Vuren\n              PJ,\n            \n            \n              Weyer\n              J,\n            \n            \n              Nel\n              LH,\n            \n            \n              Swanepoel\n              R,\n            \n            \n              Morita\n              K,\n            \n            \n              Paweska\n              JT.\n            \n          \n          2009.\n\t\t\t\t\tDevelopment and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid detection of Rift Valley fever virus in clinical specimens.\n\t\t\t\t\tJ Clin Microbiol47:\n\t\t\t\t\t645\u2013\n\t\t\t\t\t651."},{"key":"i8756-971X-37-4-256-Li1","series-title":"Virol J","first-page":"360","article-title":"Simultaneous detection and differentiation of dengue virus serotypes 1\u20134, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay","volume":"8","author":"Li","year":"2011","unstructured":"Li\n              S,\n            \n            \n              Fang\n              M,\n            \n            \n              Zhou\n              B,\n            \n            \n              Ni\n              H,\n            \n            \n              Shen\n              Q,\n            \n            \n              Zhang\n              H,\n            \n            \n              Han\n              Y,\n            \n            \n              Yin\n              J,\n            \n            \n              Chang\n              W,\n            \n            \n              Xu\n              G,\n            \n            \n              Cao\n              G.\n            \n          \n          2011.\n\t\t\t\t\tSimultaneous detection and differentiation of dengue virus serotypes 1\u20134, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay.\n\t\t\t\t\tVirol J8:\n\t\t\t\t\t360."},{"key":"i8756-971X-37-4-256-Liu1","series-title":"Vector Borne Zoonotic Dis","first-page":"1042","article-title":"Reverse transcription loop-mediated isothermal amplification for rapid detection of Japanese encephalitis virus in swine and mosquitoes","volume":"12","author":"Liu","year":"2012","unstructured":"Liu\n              H,\n            \n            \n              Liu\n              ZJ,\n            \n            \n              Jing\n              J,\n            \n            \n              Ren\n              JQ,\n            \n            \n              Liu\n              YY,\n            \n            \n              Guo\n              HH,\n            \n            \n              Fan\n              M,\n            \n            \n              Lu\n              HJ,\n            \n            \n              Jin\n              NY.\n            \n          \n          2012.\n\t\t\t\t\tReverse transcription loop-mediated isothermal amplification for rapid detection of Japanese encephalitis virus in swine and mosquitoes.\n\t\t\t\t\tVector Borne Zoonotic Dis12:\n\t\t\t\t\t1042\u2013\n\t\t\t\t\t1052."},{"key":"i8756-971X-37-4-256-McDonald1","series-title":"MMWR","first-page":"673","article-title":"West Nile virus and other domestic nationally notifiable arboviral diseases\u2014United States, 2018","volume":"68","author":"McDonald","year":"2019","unstructured":"McDonald\n              E,\n            \n            \n              Martin\n              SW,\n            \n            \n              Landry\n              K,\n            \n            \n              Gould\n              CV,\n            \n            \n              Lehman\n              J,\n            \n            \n              Fischer\n              M,\n            \n            \n              Lindsey\n              NP.\n            \n          \n          2019.\n\t\t\t\t\tWest Nile virus and other domestic nationally notifiable arboviral diseases\u2014United States, 2018.\n\t\t\t\t\tMMWR68:\n\t\t\t\t\t673\u2013\n\t\t\t\t\t678."},{"key":"i8756-971X-37-4-256-Mori1","series-title":"J Infect Chemother","first-page":"62","article-title":"Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases","volume":"15","author":"Mori","year":"2009","unstructured":"Mori\n              Y,\n            \n            \n              Notomi\n              T.\n            \n          \n          2009.\n\t\t\t\t\tLoop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases.\n\t\t\t\t\tJ Infect Chemother15:\n\t\t\t\t\t62\u2013\n\t\t\t\t\t69."},{"key":"i8756-971X-37-4-256-Notomi1","series-title":"Nucleic Acids Res","first-page":"E63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","unstructured":"Notomi\n              T,\n            \n            \n              Okayama\n              H,\n            \n            \n              Masubuchi\n              H,\n            \n            \n              Yonekawa\n              T,\n            \n            \n              Watanabe\n              K,\n            \n            \n              Amino\n              N,\n            \n            \n              Hase\n              T.\n            \n          \n          2000.\n\t\t\t\t\tLoop-mediated isothermal amplification of DNA.\n\t\t\t\t\tNucleic Acids Res28:\n\t\t\t\t\tE63."},{"key":"i8756-971X-37-4-256-Parida1","series-title":"J Clin Microbiol","first-page":"2895","article-title":"Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay","volume":"43","author":"Parida","year":"2005","unstructured":"Parida\n              M,\n            \n            \n              Horioke\n              K,\n            \n            \n              Ishida\n              H,\n            \n            \n              Dash\n              PK,\n            \n            \n              Saxena\n              P,\n            \n            \n              Jana\n              AM,\n            \n            \n              Islam\n              MA,\n            \n            \n              Inoue\n              S,\n            \n            \n              Hosaka\n              N,\n            \n            \n              Morita\n              K.\n            \n          \n          2005.\n\t\t\t\t\tRapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay.\n\t\t\t\t\tJ Clin Microbiol43:\n\t\t\t\t\t2895\u2013\n\t\t\t\t\t2903."},{"key":"i8756-971X-37-4-256-Parida2","series-title":"J Clin Microbiol","first-page":"257","article-title":"Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus","volume":"42","author":"Parida","year":"2004","unstructured":"Parida\n              M,\n            \n            \n              Posadas\n              G,\n            \n            \n              Inoue\n              S,\n            \n            \n              Hasebe\n              F,\n            \n            \n              Morita\n              K.\n            \n          \n          2004.\n\t\t\t\t\tReal-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus.\n\t\t\t\t\tJ Clin Microbiol42:\n\t\t\t\t\t257\u2013\n\t\t\t\t\t263."},{"key":"i8756-971X-37-4-256-Parida3","series-title":"Rev Med Virol","first-page":"407","article-title":"Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases","volume":"18","author":"Parida","year":"2008","unstructured":"Parida\n              M,\n            \n            \n              Sannarangaiah\n              S,\n            \n            \n              Dash\n              PK,\n            \n            \n              Rao\n              PV,\n            \n            \n              Morita\n              K.\n            \n          \n          2008.\n\t\t\t\t\tLoop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases.\n\t\t\t\t\tRev Med Virol18:\n\t\t\t\t\t407\u2013\n\t\t\t\t\t421."},{"key":"i8756-971X-37-4-256-Parida4","series-title":"J Clin Microbiol","first-page":"351","article-title":"Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay","volume":"45","author":"Parida","year":"2007","unstructured":"Parida\n              MM,\n            \n            \n              Santhosh\n              SR,\n            \n            \n              Dash\n              PK,\n            \n            \n              Tripathi\n              NK,\n            \n            \n              Lakshmi\n              V,\n            \n            \n              Mamidi\n              N,\n            \n            \n              Shrivastva\n              A,\n            \n            \n              Gupta\n              N,\n            \n            \n              Saxena\n              P,\n            \n            \n              Babu\n              JP,\n            \n            \n              Rao\n              PV,\n            \n            \n              Morita\n              K.\n            \n          \n          2007.\n\t\t\t\t\tRapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay.\n\t\t\t\t\tJ Clin Microbiol45:\n\t\t\t\t\t351\u2013\n\t\t\t\t\t357."},{"key":"i8756-971X-37-4-256-Petersen1","series-title":"J Med Entomol","first-page":"1456","article-title":"Epidemiology of West Nile virus in the United States: implications for arbovirology and public health","volume":"56","author":"Petersen","year":"2019","unstructured":"Petersen\n              L.\n            \n          \n          2019.\n\t\t\t\t\tEpidemiology of West Nile virus in the United States: implications for arbovirology and public health.\n\t\t\t\t\tJ Med Entomol56:\n\t\t\t\t\t1456\u2013\n\t\t\t\t\t1462."},{"key":"i8756-971X-37-4-256-Rosen1","series-title":"Am J Trop Med Hyg","first-page":"1153","article-title":"The use of mosquitoes to detect and propagate dengue viruses","volume":"23","author":"Rosen","year":"1974","unstructured":"Rosen\n              L,\n            \n            \n              Gubler\n              D.\n            \n          \n          1974.\n\t\t\t\t\tThe use of mosquitoes to detect and propagate dengue viruses.\n\t\t\t\t\tAm J Trop Med Hyg23:\n\t\t\t\t\t1153\u2013\n\t\t\t\t\t1160."},{"key":"i8756-971X-37-4-256-Toeh1","series-title":"BMC Infect Dis","first-page":"387","article-title":"Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification","volume":"13","author":"Toeh","year":"2013","unstructured":"Toeh\n              BT,\n            \n            \n              Sam\n              SS,\n            \n            \n              Tan\n              KK,\n            \n            \n              Johari\n              J,\n            \n            \n              Danlami\n              MB,\n            \n            \n              Hooi\n              PS,\n            \n            \n              Md-Esa\n              R,\n            \n            \n              Abubaker\n              S.\n            \n          \n          2013.\n\t\t\t\t\tDetection of dengue viruses using reverse transcription-loop-mediated isothermal amplification.\n\t\t\t\t\tBMC Infect Dis13:\n\t\t\t\t\t387."},{"key":"i8756-971X-37-4-256-Varnado1","series-title":"J Environ Health","first-page":"20","article-title":"Use of the VectorTest for advanced warning of human West Nile virus cases in Mississippi","volume":"79","author":"Varnado","year":"2016","unstructured":"Varnado\n              WC,\n            \n            \n              Goddard\n              J.\n            \n          \n          2016.\n\t\t\t\t\tUse of the VectorTest for advanced warning of human West Nile virus cases in Mississippi.\n\t\t\t\t\tJ Environ Health79:\n\t\t\t\t\t20\u2013\n\t\t\t\t\t24."},{"key":"i8756-971X-37-4-256-Wang1","series-title":"J Virol Methods","first-page":"86","article-title":"Rapid and sensitive detection of Zika virus by reverse transcription loop-mediated isothermal amplification","volume":"238","author":"Wang","year":"2016","unstructured":"Wang\n              X,\n            \n            \n              Yin\n              F,\n            \n            \n              Bi\n              Y,\n            \n            \n              Cheng\n              G,\n            \n            \n              Li\n              J,\n            \n            \n              Hou\n              L,\n            \n            \n              Li\n              Y,\n            \n            \n              Yang\n              B,\n            \n            \n              Liu\n              W,\n            \n            \n              Yang\n              L.\n            \n          \n          2016.\n\t\t\t\t\tRapid and sensitive detection of Zika virus by reverse transcription loop-mediated isothermal amplification.\n\t\t\t\t\tJ Virol Methods238:\n\t\t\t\t\t86\u2013\n\t\t\t\t\t89."},{"key":"i8756-971X-37-4-256-Waters1","series-title":"PLoS One","first-page":"e105630","article-title":"Preliminary validation of direct detection of foot-and-mouth disease virus within clinical samples using reverse transcription loop-mediated isothermal amplification coupled with a simple lateral flow device for detection","volume":"9","author":"Waters","year":"2014","unstructured":"Waters\n              RA,\n            \n            \n              Fowler\n              VL,\n            \n            \n              Armson\n              B,\n            \n            \n              Nelson\n              N,\n            \n            \n              Gloster\n              J,\n            \n            \n              Paton\n              DJ,\n            \n            \n              King\n              DP.\n            \n          \n          2014.\n\t\t\t\t\tPreliminary validation of direct detection of foot-and-mouth disease virus within clinical samples using reverse transcription loop-mediated isothermal amplification coupled with a simple lateral flow device for detection.\n\t\t\t\t\tPLoS One9:\n\t\t\t\t\te105630."},{"key":"i8756-971X-37-4-256-Watson1","series-title":"Theriogenology","first-page":"1167","article-title":"Method agreement analysis: a review of correct methodology","volume":"73","author":"Watson","year":"2010","unstructured":"Watson\n              PF,\n            \n            \n              Petrie\n              A.\n            \n          \n          2010.\n\t\t\t\t\tMethod agreement analysis: a review of correct methodology.\n\t\t\t\t\tTheriogenology73:\n\t\t\t\t\t1167\u2013\n\t\t\t\t\t1179."},{"key":"i8756-971X-37-4-256-Wheeler1","series-title":"PLoS One","first-page":"e0147962","article-title":"Surveillance for Western equine encephalitis, St. Louis encephalitis, and West Nile viruses using reverse transcription loop-mediated isothermal amplification","volume":"11","author":"Wheeler","year":"2016","unstructured":"Wheeler\n              SS,\n            \n            \n              Ball\n              CS,\n            \n            \n              Langevin\n              SA,\n            \n            \n              Fang\n              Y,\n            \n            \n              Coffey\n              LL,\n            \n            \n              Meagher\n              RJ.\n            \n          \n          2016.\n\t\t\t\t\tSurveillance for Western equine encephalitis, St. Louis encephalitis, and West Nile viruses using reverse transcription loop-mediated isothermal amplification.\n\t\t\t\t\tPLoS One11:\n\t\t\t\t\te0147962."}],"container-title":["Journal of the American Mosquito Control Association"],"original-title":[],"link":[{"URL":"https:\/\/jamca.kglmeridian.com\/view\/journals\/moco\/37\/4\/article-p256.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/meridian.allenpress.com\/jamca\/article-pdf\/37\/4\/256\/2981627\/i8756-971x-37-4-256.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/jamca.kglmeridian.com\/downloadpdf\/journals\/moco\/37\/4\/article-p256.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/jamca.kglmeridian.com\/downloadpdf\/journals\/moco\/37\/4\/article-p256.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:40:11Z","timestamp":1747356011000},"score":1,"resource":{"primary":{"URL":"https:\/\/jamca.kglmeridian.com\/view\/journals\/moco\/37\/4\/article-p256.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":30,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.2987\/21-7033","relation":{},"ISSN":["8756-971X"],"issn-type":[{"type":"print","value":"8756-971X"}],"subject":[],"published":{"date-parts":[[2021,12]]}}}