{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T13:37:43Z","timestamp":1744205863432,"version":"3.32.0"},"reference-count":34,"publisher":"American Association for Laboratory Animal Science","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:p>Enterovirus D68 (EV-D68), a respiratory RNA virus in the family Picornaviridae, is implicated as a potential etiological agent for acute flaccid myelitis in preteen adolescents. The absence of a specific therapeutic intervention necessitates the development of an effective animal model for EV-D68. The AG129 mouse strain, characterized by the double knockout of IFN-\u03b1\/\u03b2 and IFN-\u03b3 receptors on the 129 genetic background, has been proposed as a suitable model for EV-D68. The goals of this study were to assess the effect of a nonmouse-adapted EV-D68 strain (US\/MO\/14-18947, NR-49129) in AG129 (IFN-\u03b1\/\u03b2 and IFN-\u03b3 receptors null), A129 (IFN-\u03b1\/\u03b2 receptor null), G129 (IFN-\u03b3 receptor null), and the 129 background strain (129S2\/SvPasCrl) when infected intraperitoneally at 10 d of age. Both AG129 and A129 strains demonstrated similar clinical signs (paralysis, paresis, lethargy, dyspnea [characterized by prominent abdominal respiration], and morbidity requiring euthanasia) induced by EV-D68. While G129 and 129S2 strains also exhibited susceptibility to EV-D68, the severity of clinical signs was less than in AG129 and A129 strains, and many survived to the experimental endpoint. Histopathological and immunohistochemical data confirmed EV-D68 tropism for the skeletal muscle and spinal cord and suggest that the dyspnea observed in infected mice could be attributed, in part, to lesions in the diaphragmatic skeletal muscles. These findings contribute valuable insights into the pathogenesis of EV-D68 infection in this mouse model and provide investigators with key information on virus dose and mouse strain selection when using this mouse model to evaluate candidate EV-D68 therapeutics.<\/jats:p>","DOI":"10.30802\/aalas-cm-24-044","type":"journal-article","created":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T01:06:07Z","timestamp":1723683967000},"page":"352-359","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of Effect of Enterovirus D68 in 129\/Sv Mice Deficient in IFN-\u03b1\/\u03b2 and\/or IFN-\u03b3 Receptors"],"prefix":"10.30802","volume":"74","author":[{"given":"Wenqi","family":"Song","sequence":"first","affiliation":[{"name":"Department of Comparative Medicine, Stanford School of Medicine, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanya","family":"Watarastaporn","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine, Stanford School of Medicine, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaw Shin","family":"Ooi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford School of Medicine, Stanford, California; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Medicine\/Gastroenterology and Hepatology, Stanford School of Medicine, Stanford, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffery S","family":"Glenn","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford School of Medicine, Stanford, California; and"},{"name":"Department of Medicine\/Gastroenterology and Hepatology, Stanford School of Medicine, Stanford, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan E","family":"Carette","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford School of Medicine, Stanford, California; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerriann M","family":"Casey","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine, Stanford School of Medicine, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude M","family":"Nagamine","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine, Stanford School of Medicine, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"12258","reference":[{"key":"bib1","series-title":"Pathogens","first-page":"879","article-title":"AG129 mice as a comprehensive model for the experimental assessment of mosquito vector competence for arboviruses","volume":"11","author":"Baldon LVR","year":"2022","unstructured":"Baldon LVR, de Mendon\u00e7a SF, Ferreira FV, Rezende FO, Amadou SCG, Leite T, Rocha MN, Marques JT, Moreira LA, Ferreira AGA. 2022. 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