{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T22:07:51Z","timestamp":1745273271921},"reference-count":0,"publisher":"American Association for Laboratory Animal Science","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j am assoc lab anim sci"],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:p>Nest building and burrowing are highly motivated natural behaviors in rodents, and changes in these behaviors can serve as welfare assessment tools. In this study, we investigated: 1) the limits of agreement between 2 observers for a refined scoring method for nest-building behavior;\n 2) the effect of repeated exposure to 15 min of isoflurane on nest-building behavior; 3) the effect of 24 h of grid-floor housing, repeated exposure to 15 min isoflurane, and daily intraperitoneal injection of 0.2 mL 0.9% isotonic saline for 3 d on burrowing behavior; and 4) the effect of\n exposure to grid-floor housing, isoflurane, and intraperitoneal injections on fecal corticosterone metabolites, body weight, fur status, and sucrose preference in mice. SPF C57BL\/6NTac female mice (<jats:italic>n <\/jats:italic> = 27) were included in the study and were assessed first for burrowing behavior, followed\n by 2 wk of rest and then for nesting behavior. The refined scoring method for nest-building activity had good inter observer agreement. According to this method, a single exposure to anesthesia with isoflurane led to a decrease in nest-building activity and sucrose preference; a second exposure\n to anesthesia with isoflurane had no effect on nest building. Neither grid-floor housing nor repeated exposure to isoflurane anesthesia had any effect on burrowing behavior in mice. In contrast, intraperitoneal injections increased burrowing behavior. In conclusion, a refined scoring method\n for nest-building activity test that we developed for this study proved to be objective and sensitive to the effect of an initial exposure to anesthesia with isoflurane.<\/jats:p>","DOI":"10.30802\/aalas-jaalas-19-000027","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T01:06:07Z","timestamp":1578013567000},"page":"30-36","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Repeated Exposure to Isoflurane on Nest Building and Burrowing in Mice"],"prefix":"10.30802","volume":"59","author":[{"given":"Karen","family":"Gjendal","sequence":"first","affiliation":[]},{"given":"Jan L","family":"Ottesen","sequence":"additional","affiliation":[]},{"given":"I  Anna S","family":"Olsson","sequence":"additional","affiliation":[]},{"given":"Dorte B","family":"S\u00f8rensen","sequence":"additional","affiliation":[]}],"member":"12258","container-title":["Journal of the American Association for Laboratory Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/aalas\/jaalas\/2020\/00000059\/00000001\/art00005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T04:51:47Z","timestamp":1580359907000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.30802\/AALAS-JAALAS-19-000027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1,1]]}},"alternative-id":["1559-6109(20200101)59:1L.30;1-"],"URL":"https:\/\/doi.org\/10.30802\/aalas-jaalas-19-000027","relation":{},"ISSN":["1559-6109"],"issn-type":[{"type":"print","value":"1559-6109"}],"subject":[],"published":{"date-parts":[[2020,1,1]]}}}