{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:20:41Z","timestamp":1783426841583,"version":"3.54.6"},"reference-count":28,"publisher":"IMR Press","issue":"2","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Elite Ed)"],"abstract":"<jats:p>Background: This study aimed to improve the cassava retting efficiency of starter Sta_96, a fermented cassava flour obtained after four days of fermentation, by incorporating bacteria of the genus Streptomyces. Methods: Streptomyces isolates were obtained from Sta_96, farm soils, and a cassava peel dump. The subsequent pectinolytic and cellulolytic activities, along with the associated ability to soften cassava roots, were evaluated. The most effective isolates were incorporated into Sta_96 at a concentration of 106 CFU\/g, and the associated retting and detoxification efficiencies were assessed. Results: Of the 33 Streptomyces isolates recovered, 24 produced pectinases, with hydrolysis halos ranging from 11.0 \u00b1 1.4 mm to 42.5 \u00b1 3.5 mm. Sixteen of these displayed cellulase activity, with halos ranging from 16.0 \u00b1 0.1 mm to 40.0 \u00b1 0.1 mm. STF 16 showed the highest softening efficiency, achieving a retting time of 38.6 \u00b1 0.4 h, and significantly improved the performance of Sta_96 by reducing the retting duration from 44.5 \u00b1 0.6 h to 24.3 \u00b1 0.4 h, representing a 45.39% reduction. STF 16 also demonstrated substantial detoxification efficiency (~85%). Conclusions: This is the first report demonstrating that Streptomyces species can act as efficient cassava retting agents. Incorporation of STF 16 into the Sta_96 starter markedly accelerated root softening to about 24 hours while ensuring complete elimination of cyanide. This approach represents a promising strategy for improving fermentation efficiency and product safety in cassava-processing industries.<\/jats:p>","DOI":"10.31083\/fbe45577","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:01:18Z","timestamp":1783425678000},"source":"Crossref","is-referenced-by-count":0,"title":["Enhancement of Cassava (Manihot esculenta Crantz) Retting Efficiency Using Pectinolytic and Cellulolytic Streptomyces sp. in the Traditional Starter Sta_96"],"prefix":"10.31083","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1886-6095","authenticated-orcid":true,"given":"Didier-Olivier","family":"B\u00e9bin\u00e9 Bi\u00e9di","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Science, University of Yaound\u00e9 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3236-6079","authenticated-orcid":true,"given":"Nardis","family":"Nkoudou Ze","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science, University of Yaound\u00e9 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3289-6841","authenticated-orcid":true,"given":"Marielle Neisha","family":"Belinga Atangana","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science, University of Yaound\u00e9 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-9064","authenticated-orcid":true,"given":"Eliane Flore","family":"Eyenga","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, University of Yaound\u00e9 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9940-7449","authenticated-orcid":true,"given":"Jean Justin","family":"Essia Ngang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science, University of Yaound\u00e9 1, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Bayata A. 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