{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T09:40:09Z","timestamp":1768902009094,"version":"3.49.0"},"reference-count":34,"publisher":"IMR Press","issue":"1","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: Over the past five years, the pregnancy rate in assisted reproductive technology (ART) programs in Russia has remained relatively stable. The aim of this study was to assess the distribution of monocyte and macrophage subsets in the blood and follicular fluid of infertile women undergoing assisted reproductive technology. Methods: The study involved 45 women with a mean age of 35 \u00b1 4.66 years. Monocytes and macrophages were identified using flow cytometry. Results: We observed a decrease in the CD68+CD163+CD206+ and the CD68+CD163\u2013CD206+ cells in patients with a body mass index (BMI) &gt;25 by 0.19 times and 6.56 times, respectively, compared to the group with a BMI &lt;25 (p = 0.031). Patients with fair oocyte quality had 3.6 times more oocytes than those with poor quality (p = 0.010). The relative content of CD14+163\u2013206+ monocytes was found to be 24.15 times higher in the follicular fluid of women with poor embryo quality compared to the group with good embryos (p = 0.010). We also noted that the number of oocytes increased in women with male factor infertility (p = 0.020) and those with unspecified infertility when compared to tubal infertility. An increase in the relative content of CD14+163+206+ in the blood was higher in women with other causes of female infertility compared to those with male factor infertility (p = 0.010). The relative content of M2-monocytes (CD14+163+206\u2013) in the blood was 4.38 times higher in women with male factor infertility than in women with unexplained infertility (p = 0.010). Conclusions: A critical component of the inflammatory reaction in patients undergoing in vitro fertilization (IVF) involves more than just the activation of pro-inflammatory cells in response to ovarian stimulation. Our research shows that changes in the distribution of monocytes and macrophages can influence embryo implantation success and pregnancy outcomes in women. These processes are influenced by various infertility-related factors, including those mentioned above. However, these findings are preliminary and require further investigation.<\/jats:p>","DOI":"10.31083\/fbl26660","type":"journal-article","created":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T08:54:32Z","timestamp":1737795272000},"source":"Crossref","is-referenced-by-count":1,"title":["Monocyte and Macrophage in Follicular Liquid: Predictive Markers of Embryo Quality in Women with Obesity and Infertility"],"prefix":"10.31083","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7082-9389","authenticated-orcid":true,"given":"Evgeny D.","family":"Merkulov","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Molecular Biology, Federal State Budgetary Educational Institution of Higher Education \u201cSiberian State Medical University\u201d of the Ministry of Health of Russia, 634050 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8069-4038","authenticated-orcid":true,"given":"Anastasia V.","family":"Prozorova","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Molecular Biology, Federal State Budgetary Educational Institution of Higher Education \u201cSiberian State Medical University\u201d of the Ministry of Health of Russia, 634050 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2667-4842","authenticated-orcid":true,"given":"Iuliia G.","family":"Samoilova","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Molecular Biology, Federal State Budgetary Educational Institution of Higher Education \u201cSiberian State Medical University\u201d of the Ministry of Health of Russia, 634050 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8985-009X","authenticated-orcid":true,"given":"Dmitry A.","family":"Svarovsky","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Molecular Biology, Federal State Budgetary Educational Institution of Higher Education \u201cSiberian State Medical University\u201d of the Ministry of Health of Russia, 634050 Tomsk, Russia"},{"name":"Laboratory of Tumor Biochemistry, Cancer Research Institute, Tomsk National Research Medical Center, 634009 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6263-6716","authenticated-orcid":true,"given":"Kira A.","family":"Sidorenkova","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Molecular Biology, Federal State Budgetary Educational Institution of Higher Education \u201cSiberian State Medical University\u201d of the Ministry of Health of Russia, 634050 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5269-736X","authenticated-orcid":true,"given":"Liudmila V.","family":"Spirina","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Molecular Biology, Federal State Budgetary Educational Institution of Higher Education \u201cSiberian State Medical University\u201d of the Ministry of Health of Russia, 634050 Tomsk, Russia"},{"name":"Laboratory of Tumor Biochemistry, Cancer Research Institute, Tomsk National Research Medical Center, 634009 Tomsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0601-2240","authenticated-orcid":true,"given":"Marina N.","family":"Stakheeva","sequence":"first","affiliation":[{"name":"Division of Biochemistry and Molecular Biology, Federal State Budgetary Educational Institution of Higher Education \u201cSiberian State Medical University\u201d of the Ministry of Health of Russia, 634050 Tomsk, Russia"},{"name":"Laboratory of Tumor Biochemistry, Cancer Research Institute, Tomsk National Research Medical Center, 634009 Tomsk, 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Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization. International Journal of Molecular Sciences. 2021; 22: 8735.","DOI":"10.3390\/ijms22168735."},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Martynova AE. Possibilities for predicting the effectiveness of assisted reproductive technology programs. Obstetrics and Gynecology. 2022; 9: 26\u201332.","DOI":"10.18565\/aig.2022.9.26-32."},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Sciorio R, Tramontano L, Bellaminutti S, Aiello R, Fortunato A, Marci R, et al. Review of the impact of COVID-19 on male reproduction, and its implications on assisted reproductive technology services. Zygote (Cambridge, England). 2022; 30: 743\u2013748.","DOI":"10.1017\/S0967199421000666."},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Zhang D, Yu Y, Duan T, Zhou Q. The role of macrophages in reproductive-related diseases. 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Differential immunophenotype of circulating monocytes from pregnant women in response to viral ligands. BMC Pregnancy and Childbirth. 2023; 23: 323.","DOI":"10.1186\/s12884-023-05562-0."},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Faas MM, de Vos P. Maternal monocytes in pregnancy and preeclampsia in humans and in rats. Journal of Reproductive Immunology. 2017; 119: 91\u201397.","DOI":"10.1016\/j.jri.2016.06.009."},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Kasum M, Ore\u0161kovi\u0107 S, \u010cehi\u0107 E, Lila A, Ejubovi\u0107 E, Soldo D. The role of female obesity on in vitro fertilization outcomes. Gynecological Endocrinology: the Official Journal of the International Society of Gynecological Endocrinology. 2018; 34: 184\u2013188.","DOI":"10.1080\/09513590.2017.1391209."},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Fedorov AA, Ermak NA, Gerashchenko TS, Topolnitskiy EB, Shefer NA, Rodionov EO, et al. Polarization of macrophages: mechanisms, markers and factors of induction. Siberian Journal of Oncology. 2022; 21: 124\u2013136.","DOI":"10.21294\/1814-4861-2022-21-4-124-136."},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Herrero Y, Pascuali N, Vel\u00e1zquez C, Oubi\u00f1a G, Hauk V, de Z\u00fa\u00f1iga I, et al. SARS-CoV-2 infection negatively affects ovarian function in ART patients. Biochimica et Biophysica Acta. Molecular Basis of Disease. 2022; 1868: 166295.","DOI":"10.1016\/j.bbadis.2021.166295."},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Ribot E, Berbis J, Hamouda I, Cohen D, Agostini A, Courbiere B. Pregnancy outcomes after in vitro fertilization for moderate and severe endometriosis. A case-control study. Journal of Gynecology Obstetrics and Human Reproduction. 2022; 51: 102274.","DOI":"10.1016\/j.jogoh.2021.102274."},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Tian X, Eikmans M, Hoorn MLVD. The Role of Macrophages in Oocyte Donation Pregnancy: A Systematic Review. International Journal of Molecular Sciences. 2020; 21: 939.","DOI":"10.3390\/ijms21030939."},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Berezhna VA, Mamontova TV, Gromova AM. CD68+ M1 macrophages is associated with placental insufficiency under fetal growth restriction. Wiadomosci Lekarskie (Warsaw, Poland: 1960). 2021; 74: 213\u2013219.","DOI":"10.36740\/WLek202102107"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Ersoy Canillioglu Y, Senturk GE, Sahin H, Sahin S, Seval-Celik Y. The Distribution of Foxp3 and CD68 in Preeclamptic and Healthy Placentas: A Histomorphological Evaluation. The Iournal of Histochemistry and Cytochemistry : Official Journal of The Histochemistry Society. 2023; 71: 211-225.","DOI":"10.1369\/00221554231170662."},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Akbar MIA, Azis MA, Riu DS, Wawengkang E, Ernawati E, Bachnas MA, et al. INOVASIA Study: A Multicenter Randomized Clinical Trial of Pravastatin to Prevent Preeclampsia in High-Risk Patients. American Journal of Perinatology. 2024; 41: 1203\u20131211.","DOI":"10.1055\/a-1798-1925."},{"key":"ref25","unstructured":"Khachaturyan DA, Smolnikova VYu, Burmenskaya OV, Donnikov AE, Nepsha OS. Proinflammatory gene expression profile of innate immunity in the vaginal mucosa as a predictor of the outcome of an in vitro fertilization and embryo transfer program. Obstetrics and Gynecology. 2015; 4: 50\u201355. (In Russian)."},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"Krow-Lucal ER, Kim CC, Burt TD, McCune JM. Distinct functional programming of human fetal and adult monocytes. Blood. 2014; 123: 1897\u20131904.","DOI":"10.1182\/blood-2013-11-536094."},{"key":"ref27","doi-asserted-by":"publisher","unstructured":"Paparini DE, Grasso E, Fernandez LDC, Merech F, Weingrill-Barbano R, Correa-Silva S, et al. Decidual factors and vasoactive intestinal peptide guide monocytes to higher migration, efferocytosis and wound healing in term human pregnancy. Acta Physiologica (Oxford, England). 2021; 232: e13579.","DOI":"10.1111\/apha.13579."},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Zhang C, Li T, Yin S, Gao M, He H, Li Y, et al. Monocytes deposit migrasomes to promote embryonic angiogenesis. Nature Cell Biology. 2022; 24: 1726\u20131738.","DOI":"10.1038\/s41556-022-01026-3."},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW, Jr. Obesity is associated with macrophage accumulation in adipose tissue. The Journal of Clinical Investigation. 2003; 112: 1796\u20131808.","DOI":"10.1172\/JCI19246."},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Luo T, Wang M, He Y, Zhang M, Liu B, Li W, et al. Metabolic profile of follicular fluid in patients with ovarian endometriosis undergoing IVF: a pilot study. Reproductive Biomedicine Online. 2024; 49: 103912.","DOI":"10.1016\/j.rbmo.2024.103912."},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"Horta F, Catt S, Ramachandran P, Vollenhoven B, Temple-Smith P. Female ageing affects the DNA repair capacity of oocytes in IVF using a controlled model of sperm DNA damage in mice. Human Reproduction (Oxford, England). 2020; 35: 529\u2013544.","DOI":"10.1093\/humrep\/dez308."},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"Yang X, Gilman-Sachs A, Kwak-Kim J. Ovarian and endometrial immunity during the ovarian cycle. Journal of Reproductive Immunology. 2019; 133: 7\u201314.","DOI":"10.1016\/j.jri.2019.04.001."},{"key":"ref33","doi-asserted-by":"publisher","unstructured":"Wei X, Yang X. The central role of natural killer cells in preeclampsia. Frontiers in Immunology. 2023; 14: 1009867.","DOI":"10.3389\/fimmu.2023.1009867."},{"key":"ref34","doi-asserted-by":"publisher","unstructured":"Luk\u00e1cs L, Kov\u00e1cs AR, P\u00e1l L, Sz\u0171cs S, K\u00f6v\u00e9r \u00c1, Lamp\u00e9 R. Phagocyte function of peripheral neutrophil granulocytes and monocytes in endometriosis before and after surgery. Journal of Gynecology Obstetrics and Human Reproduction. 2021; 50: 101796.","DOI":"10.1016\/j.jogoh.2020.101796."}],"container-title":["Frontiers in Bioscience-Landmark"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/30\/1\/10.31083\/FBL26660","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T09:07:49Z","timestamp":1737796069000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/30\/1\/10.31083\/FBL26660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,22]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025]]}},"alternative-id":["S2768-6701(24)01581-8"],"URL":"https:\/\/doi.org\/10.31083\/fbl26660","relation":{},"ISSN":["2768-6701","2768-6698"],"issn-type":[{"value":"2768-6701","type":"print"},{"value":"2768-6698","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,22]]},"article-number":"26660"}}