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(Landmark Ed)"],"abstract":"<jats:p>Background: Alzheimer\u2019s disease (AD) is a severe neurodegenerative disorder characterized by beta-amyloid plaques and tau neurofibrillary tangles. The diagnosis of AD is complex, with the analysis of beta-amyloid and tau in cerebrospinal fluid being a well-established diagnostic approach. However, currently no blood biomarkers have been identified or validated for clinical use. In the present study, we will identify novel plasma biomarkers for AD using our well-established organotypic mouse brain slice model connected to microcontact prints. We hypothesize that AD plasma contains factors that affect endothelial cell migration and new vessel formation. Methods: In the present study, plasma from human patients is microcontact printed and connected to mouse brain slices. After 4 weeks in culture, laminin+ and lectin+ endothelial cells (ECs) and vessels are analyzed by immunostaining techniques. The most promising samples were processed by differential mass spectrometry. Results: Our data show that AD plasma significantly increased the migration length of laminin+ and lectin+ ECs along the microcontact prints. Using differential mass spectrometry, we could identify three potential biomarkers: C-reactive protein, basigin, and trem-like transcript 1 protein. Conclusion: Here we show that brain slices connected to human plasma prints allow the identification of novel human AD biomarkers with subsequent mass spectrometry. This technique represents a novel and innovative approach to translate research findings from mouse models to human applications.<\/jats:p>","DOI":"10.31083\/fbl36257","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T12:04:10Z","timestamp":1743163450000},"source":"Crossref","is-referenced-by-count":3,"title":["Novel Plasma Biomarkers for Alzheimer\u2019s Disease: Insights from Organotypic Brain Slice and Microcontact Printing Techniques"],"prefix":"10.31083","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1759-3052","authenticated-orcid":true,"given":"Sakir Necat","family":"Yilmaz","sequence":"first","affiliation":[{"name":"Laboratory of Psychiatry and Experimental Alzheimer\u2019s Research, Medical University of Innsbruck, 6020 Innsbruck, Austria"},{"name":"Department of Histology and Embryology, Faculty of Medicine, Mersin University, 33343 Mersin, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2449-4885","authenticated-orcid":true,"given":"Katharina","family":"Steiner","sequence":"first","affiliation":[{"name":"Laboratory of Psychiatry and Experimental Alzheimer\u2019s Research, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Marksteiner","sequence":"first","affiliation":[{"name":"Department of Psychiatry and Psychotherapy A, Hall State Hospital, 6060 Hall in Tirol, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Faserl","sequence":"first","affiliation":[{"name":"Protein Core Facility, Institute of Medical Biochemistry, CCB-Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4785-8739","authenticated-orcid":true,"given":"Bettina","family":"Sarg","sequence":"first","affiliation":[{"name":"Protein Core Facility, Institute of Medical Biochemistry, CCB-Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7641-4240","authenticated-orcid":true,"given":"Christian","family":"Humpel","sequence":"first","affiliation":[{"name":"Laboratory of Psychiatry and Experimental Alzheimer\u2019s Research, Medical University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2025,3,24]]},"reference":[{"key":"ref1","unstructured":"Global status report on the public health response to dementia. 2021. Available at: https:\/\/www.who.int\/publications\/i\/item\/9789240033245 (Accessed 28 November 2024)."},{"key":"ref2","unstructured":"World Health Organization. Global action plan on the public health response to dementia 2017\u20132025. World Health Organization: Geneva. 2017."},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Mulder C, Verwey NA, van der Flier WM, Bouwman FH, Kok A, van Elk EJ, et al. Amyloid-beta(1-42), total tau, and phosphorylated tau as cerebrospinal fluid biomarkers for the diagnosis of Alzheimer disease. Clinical Chemistry. 2010; 56: 248\u2013253.","DOI":"10.1373\/clinchem.2009.130518."},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Jack CR, Jr, Andrews JS, Beach TG, Buracchio T, Dunn B, Graf A, et al. Revised criteria for diagnosis and staging of Alzheimer\u2019s disease: Alzheimer\u2019s Association Workgroup. Alzheimer\u2019s & Dementia. 2024; 20: 5143\u20135169.","DOI":"10.1002\/alz.13859."},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Ray S, Britschgi M, Herbert C, Takeda-Uchimura Y, Boxer A, Blennow K, et al. Classification and prediction of clinical Alzheimer\u2019s diagnosis based on plasma signaling proteins. Nature Medicine. 2007; 13: 1359\u20131362.","DOI":"10.1038\/nm1653."},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Bj\u00f6rkqvist M, Ohlsson M, Minthon L, Hansson O. Evaluation of a previously suggested plasma biomarker panel to identify Alzheimer\u2019s disease. PLoS ONE. 2012; 7: e29868.","DOI":"10.1371\/journal.pone.0029868."},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Kaneko N, Yamamoto R, Sato TA, Tanaka K. Identification and quantification of amyloid beta-related peptides in human plasma using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry. Proceedings of the Japan Academy. Series B, Physical and Biological Sciences. 2014; 90: 104\u2013117.","DOI":"10.2183\/pjab.90.104."},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Janelidze S, Mattsson N, Palmqvist S, Smith R, Beach TG, Serrano GE, et al. Plasma P-tau181 in Alzheimer\u2019s disease: relationship to other biomarkers, differential diagnosis, neuropathology and longitudinal progression to Alzheimer\u2019s dementia. Nature Medicine. 2020; 26: 379\u2013386.","DOI":"10.1038\/s41591-020-0755-1."},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Palmqvist S, Janelidze S, Quiroz YT, Zetterberg H, Lopera F, Stomrud E, et al. Discriminative Accuracy of Plasma Phospho-tau217 for Alzheimer Disease vs Other Neurodegenerative Disorders. JAMA. 2020; 324: 772\u2013781.","DOI":"10.1001\/jama.2020.12134."},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Varesi A, Carrara A, Pires VG, Floris V, Pierella E, Savioli G, et al. Blood-Based Biomarkers for Alzheimer\u2019s Disease Diagnosis and Progression: An Overview. Cells. 2022; 11: 1367.","DOI":"10.3390\/cells11081367."},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Gustaw-Rothenberg KA, Siedlak SL, Bonda DJ, Lerner A, Tabaton M, Perry G, et al. Dissociated amyloid-beta antibody levels as a serum biomarker for the progression of Alzheimer\u2019s disease: a population-based study. Experimental Gerontology. 2010; 45: 47\u201352.","DOI":"10.1016\/j.exger.2009.10.003."},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Weldon Furr J, Morales-Scheihing D, Manwani B, Lee J, McCullough LD. Cerebral Amyloid Angiopathy, Alzheimer\u2019s Disease and MicroRNA: miRNA as Diagnostic Biomarkers and Potential Therapeutic Targets. Neuromolecular Medicine. 2019; 21: 369\u2013390.","DOI":"10.1007\/s12017-019-08568-0."},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Kumar P, Dezso Z, MacKenzie C, Oestreicher J, Agoulnik S, Byrne M, et al. Circulating miRNA biomarkers for Alzheimer\u2019s disease. PLoS ONE. 2013; 8: e69807.","DOI":"10.1371\/journal.pone.0069807."},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Kiko T, Nakagawa K, Tsuduki T, Furukawa K, Arai H, Miyazawa T. MicroRNAs in plasma and cerebrospinal fluid as potential markers for Alzheimer\u2019s disease. Journal of Alzheimer\u2019s Disease. 2014; 39: 253\u2013259.","DOI":"10.3233\/JAD-130932."},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Bronzuoli MR, Iacomino A, Steardo L, Scuderi C. Targeting neuroinflammation in Alzheimer\u2019s disease. Journal of Inflammation Research. 2016; 9: 199\u2013208.","DOI":"10.2147\/JIR.S86958."},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Humpel C. Identifying and validating biomarkers for Alzheimer\u2019s disease. Trends in Biotechnology. 2011; 29: 26\u201332.","DOI":"10.1016\/j.tibtech.2010.09.007."},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Grammas P. Neurovascular dysfunction, inflammation and endothelial activation: implications for the pathogenesis of Alzheimer\u2019s disease. Journal of Neuroinflammation. 2011; 8: 26.","DOI":"10.1186\/1742-2094-8-26."},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Govindpani K, McNamara LG, Smith NR, Vinnakota C, Waldvogel HJ, Faull RL, et al. Vascular Dysfunction in Alzheimer\u2019s Disease: A Prelude to the Pathological Process or a Consequence of It? Journal of Clinical Medicine. 2019; 8: 651.","DOI":"10.3390\/jcm8050651."},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Miyakawa T. Vascular pathology in Alzheimer\u2019s disease. Psychogeriatrics. 2010; 10: 39\u201344.","DOI":"10.1111\/j.1479-8301.2009.00294.x."},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Cortes-Canteli M, Iadecola C. Alzheimer\u2019s Disease and Vascular Aging: JACC Focus Seminar. Journal of the American College of Cardiology. 2020; 75: 942\u2013951.","DOI":"10.1016\/j.jacc.2019.10.062."},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"de la Torre JC, Mussivand T. Can disturbed brain microcirculation cause Alzheimer\u2019s disease? Neurological Research. 1993; 15: 146\u2013153.","DOI":"10.1080\/01616412.1993.11740127."},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"de la Torre J. The Vascular Hypothesis of Alzheimer\u2019s Disease: A Key to Preclinical Prediction of Dementia Using Neuroimaging. Journal of Alzheimer\u2019s Disease. 2018; 63: 35\u201352.","DOI":"10.3233\/JAD-180004."},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Hays CC, Zlatar ZZ, Wierenga CE. 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Amyloid beta peptide-induced cerebral endothelial cell death involves mitochondrial dysfunction and caspase activation. Journal of Cerebral Blood Flow and Metabolism. 2001; 21: 702\u2013710.","DOI":"10.1097\/00004647-200106000-00008."},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Davis J, Cribbs DH, Cotman CW, Van Nostrand WE. Pathogenic amyloid beta-protein induces apoptosis in cultured human cerebrovascular smooth muscle cells. Amyloid. 1999; 6: 157\u2013164.","DOI":"10.3109\/13506129909007321."},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"Jefferies WA, Price KA, Biron KE, Fenninger F, Pfeifer CG, Dickstein DL. Adjusting the compass: new insights into the role of angiogenesis in Alzheimer\u2019s disease. Alzheimer\u2019s Research & Therapy. 2013; 5: 64.","DOI":"10.1186\/alzrt230."},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"Tarkowski E, Issa R, Sj\u00f6gren M, Wallin A, Blennow K, Tarkowski A, et al. Increased intrathecal levels of the angiogenic factors VEGF and TGF-beta in Alzheimer\u2019s disease and vascular dementia. Neurobiology of Aging. 2002; 23: 237\u2013243.","DOI":"10.1016\/s0197-4580(01)00285-8."},{"key":"ref33","doi-asserted-by":"publisher","unstructured":"Bu\u00e9e L, Hof PR, Delacourte A. Brain microvascular changes in Alzheimer\u2019s disease and other dementias. Annals of the New York Academy of Sciences. 1997; 826: 7\u201324.","DOI":"10.1111\/j.1749-6632.1997.tb48457.x."},{"key":"ref34","doi-asserted-by":"publisher","unstructured":"Perlmutter LS, Chui HC, Saperia D, Athanikar J. Microangiopathy and the colocalization of heparan sulfate proteoglycan with amyloid in senile plaques of Alzheimer\u2019s disease. Brain Research. 1990; 508: 13\u201319.","DOI":"10.1016\/0006-8993(90)91111-s."},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Sundberg C, Nagy JA, Brown LF, Feng D, Eckelhoefer IA, Manseau EJ, et al. Glomeruloid microvascular proliferation follows adenoviral vascular permeability factor\/vegf-164 gene delivery. The American Journal of Pathology. 2001; 158: 1145\u20131160.","DOI":"10.1016\/S0002-9440(10)64062-X."},{"key":"ref36","doi-asserted-by":"publisher","unstructured":"Salminen A. Hypoperfusion is a potential inducer of immunosuppressive network in Alzheimer\u2019s disease. Neurochemistry International. 2021; 142: 104919.","DOI":"10.1016\/j.neuint.2020.104919."},{"key":"ref37","doi-asserted-by":"publisher","unstructured":"Erickson MA, Banks WA. Blood-brain barrier dysfunction as a cause and consequence of Alzheimer\u2019s disease. Journal of Cerebral Blood Flow and Metabolism. 2013; 33: 1500\u20131513.","DOI":"10.1038\/jcbfm.2013.135."},{"key":"ref38","doi-asserted-by":"publisher","unstructured":"Vagnucci AH, Jr, Li WW. Alzheimer\u2019s disease and angiogenesis. Lancet. 2003; 361: 605\u2013608.","DOI":"10.1016\/S0140-6736(03)12521-4."},{"key":"ref39","doi-asserted-by":"publisher","unstructured":"Humpel C. Chronic mild cerebrovascular dysfunction as a cause for Alzheimer\u2019s disease? Experimental Gerontology. 2011; 46: 225\u2013232.","DOI":"10.1016\/j.exger.2010.11.032."},{"key":"ref40","doi-asserted-by":"publisher","unstructured":"Iturria-Medina Y, Sotero RC, Toussaint PJ, Mateos-P\u00e9rez JM, Evans AC, Alzheimer\u2019s Disease Neuroimaging Initiative. Early role of vascular dysregulation on late-onset Alzheimer\u2019s disease based on multifactorial data-driven analysis. Nature Communications. 2016; 7: 11934.","DOI":"10.1038\/ncomms11934."},{"key":"ref41","doi-asserted-by":"publisher","unstructured":"Zhang YL, Wang J, Zhang ZN, Su Q, Guo JH. The relationship between amyloid-beta and brain capillary endothelial cells in Alzheimer\u2019s disease. Neural Regeneration Research. 2022; 17: 2355\u20132363.","DOI":"10.4103\/1673-5374.335829."},{"key":"ref42","doi-asserted-by":"publisher","unstructured":"Yamazaki Y, Kanekiyo T. Blood-Brain Barrier Dysfunction and the Pathogenesis of Alzheimer\u2019s Disease. International Journal of Molecular Sciences. 2017; 18: 1965.","DOI":"10.3390\/ijms18091965."},{"key":"ref43","doi-asserted-by":"publisher","unstructured":"Nelson AR, Sweeney MD, Sagare AP, Zlokovic BV. Neurovascular dysfunction and neurodegeneration in dementia and Alzheimer\u2019s disease. Biochimica et Biophysica Acta. 2016; 1862: 887\u2013900.","DOI":"10.1016\/j.bbadis.2015.12.016."},{"key":"ref44","doi-asserted-by":"publisher","unstructured":"Solis E, Jr., Hascup KN, Hascup ER. Alzheimer\u2019s Disease: The Link Between Amyloid-\u03b2 and Neurovascular Dysfunction. Journal of Alzheimer\u2019s Disease. 2020; 76: 1179\u20131198.","DOI":"10.3233\/JAD-200473."},{"key":"ref45","doi-asserted-by":"publisher","unstructured":"Zuliani G, Cavalieri M, Galvani M, Passaro A, Munari MR, Bosi C, et al. Markers of endothelial dysfunction in older subjects with late onset Alzheimer\u2019s disease or vascular dementia. Journal of the Neurological Sciences. 2008; 272: 164\u2013170.","DOI":"10.1016\/j.jns.2008.05.020."},{"key":"ref46","doi-asserted-by":"publisher","unstructured":"Kalaria RN, Hedera P. Differential degeneration of the cerebral microvasculature in Alzheimer\u2019s disease. Neuroreport. 1995; 6: 477\u2013480.","DOI":"10.1097\/00001756-199502000-00018."},{"key":"ref47","doi-asserted-by":"publisher","unstructured":"De Caterina R, Massaro M, Scoditti E, Annunziata Carluccio M. Pharmacological modulation of vascular inflammation in atherothrombosis. Annals of the New York Academy of Sciences. 2010; 1207: 23\u201331.","DOI":"10.1111\/j.1749-6632.2010.05784.x."},{"key":"ref48","doi-asserted-by":"publisher","unstructured":"Grammas P, Moore P, Weigel PH. Microvessels from Alzheimer\u2019s disease brains kill neurons in vitro. The American Journal of Pathology. 1999; 154: 337\u2013342.","DOI":"10.1016\/S0002-9440(10)65280-7."},{"key":"ref49","doi-asserted-by":"publisher","unstructured":"Dixelius J, Jakobsson L, Genersch E, Bohman S, Ekblom P, Claesson-Welsh L. Laminin-1 promotes angiogenesis in synergy with fgf by distinct regulation of the gene and protein expression profile in endothelial cells. The Journal of Biological Chemistry. 2004; 279: 23766\u201323772.","DOI":"10.1074\/jbc.M311675200."},{"key":"ref50","doi-asserted-by":"publisher","unstructured":"Yousif LF, Di Russo J, Sorokin L. Laminin isoforms in endothelial and perivascular basement membranes. Cell Adhesion & Migration. 2013; 7: 101\u2013110.","DOI":"10.4161\/cam.22680."},{"key":"ref51","doi-asserted-by":"publisher","unstructured":"Humpel C. Organotypic brain slice cultures: A review. Neuroscience. 2015; 305: 86\u201398.","DOI":"10.1016\/j.neuroscience.2015.07.086."},{"key":"ref52","doi-asserted-by":"publisher","unstructured":"Steiner K, Humpel C. Effects of Ischemia on the Migratory Capacity of Microglia Along Collagen Microcontact Prints on Organotypic Mouse Cortex Brain Slices. Frontiers in Cellular Neuroscience. 2022; 16: 858802.","DOI":"10.3389\/fncel.2022.858802."},{"key":"ref53","doi-asserted-by":"publisher","unstructured":"Steiner K, Yilmaz SN, Gern A, Marksteiner J, Faserl K, Villunger M, et al. From Organotypic Mouse Brain Slices to Human Alzheimer Plasma Biomarkers: A Focus on Microglia. Biomolecules. 2024; 14: 1109.","DOI":"10.3390\/biom14091109."},{"key":"ref54","doi-asserted-by":"publisher","unstructured":"Steiner K, Humpel C. Beta-Amyloid Enhances Vessel Formation in Organotypic Brain Slices Connected to Microcontact Prints. Biomolecules. 2023; 14: 3.","DOI":"10.3390\/biom14010003."},{"key":"ref55","doi-asserted-by":"publisher","unstructured":"Yilmaz SN, Steiner K, Marksteiner J, Faserl K, Villunger M, Sarg B, et al. From Organotypic Mouse Brain Slices to Human Alzheimer\u2019s Plasma Biomarkers: A Focus on Nerve Fiber Outgrowth. Biomolecules. 2024; 14: 1326.","DOI":"10.3390\/biom14101326."},{"key":"ref56","doi-asserted-by":"publisher","unstructured":"Kov\u00e1cs R, Papageorgiou I, Heinemann U. Slice cultures as a model to study neurovascular coupling and blood brain barrier in vitro. Cardiovascular Psychiatry and Neurology. 2011; 2011: 646958.","DOI":"10.1155\/2011\/646958."},{"key":"ref57","doi-asserted-by":"publisher","unstructured":"Bendfeldt K, Radojevic V, Kapfhammer J, Nitsch C. 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Long-term organotypic brain slices cultured on collagen-based microcontact prints: A perspective for a brain-on-a-chip. Journal of Neuroscience Methods. 2023; 399: 109979.","DOI":"10.1016\/j.jneumeth.2023.109979."},{"key":"ref66","doi-asserted-by":"publisher","unstructured":"Schmidt O, Weyer Y, Baumann V, Widerin MA, Eising S, Angelova M, et al. Endosome and Golgi-associated degradation (EGAD) of membrane proteins regulates sphingolipid metabolism. The EMBO Journal. 2019; 38: e101433.","DOI":"10.15252\/embj.2018101433."},{"key":"ref67","doi-asserted-by":"publisher","unstructured":"Indelicato E, Faserl K, Amprosi M, Nachbauer W, Schneider R, Wanschitz J, et al. Skeletal muscle proteome analysis underpins multifaceted mitochondrial dysfunction in Friedreich\u2019s ataxia. 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Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. The Journal of Cell Biology. 1988; 107: 1589\u20131598.","DOI":"10.1083\/jcb.107.4.1589."},{"key":"ref82","doi-asserted-by":"publisher","unstructured":"Stamati K, Priestley JV, Mudera V, Cheema U. Laminin promotes vascular network formation in 3D in vitro collagen scaffolds by regulating VEGF uptake. Experimental Cell Research. 2014; 327: 68\u201377.","DOI":"10.1016\/j.yexcr.2014.05.012."},{"key":"ref83","doi-asserted-by":"publisher","unstructured":"Simon-Assmann P, Orend G, Mammadova-Bach E, Spenl\u00e9 C, Lefebvre O. Role of laminins in physiological and pathological angiogenesis. The International Journal of Developmental Biology. 2011; 55: 455\u2013465.","DOI":"10.1387\/ijdb.103223ps."},{"key":"ref84","doi-asserted-by":"publisher","unstructured":"Sch\u00e9ele S, Sasaki T, Arnal-Estap\u00e9 A, Durbeej M, Ekblom P. Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain. Matrix Biology. 2006; 25: 301\u2013305.","DOI":"10.1016\/j.matbio.2006.03.004."},{"key":"ref85","doi-asserted-by":"publisher","unstructured":"Maciag T, Kadish J, Wilkins L, Stemerman MB, Weinstein R. Organizational behavior of human umbilical vein endothelial cells. The Journal of Cell Biology. 1982; 94: 511\u2013520.","DOI":"10.1083\/jcb.94.3.511."},{"key":"ref86","doi-asserted-by":"publisher","unstructured":"Madri JA, Williams SK. Capillary endothelial cell cultures: phenotypic modulation by matrix components. The Journal of Cell Biology. 1983; 97: 153\u2013165.","DOI":"10.1083\/jcb.97.1.153."},{"key":"ref87","doi-asserted-by":"publisher","unstructured":"Raposo CD, Canelas AB, Barros MT. Human Lectins, Their Carbohydrate Affinities and Where to Find Them. 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Journal of the Neurological Sciences. 2012; 322: 117\u2013121.","DOI":"10.1016\/j.jns.2012.07.010."},{"key":"ref103","doi-asserted-by":"publisher","unstructured":"De Smet F, Segura I, De Bock K, Hohensinner PJ, Carmeliet P. Mechanisms of vessel branching: filopodia on endothelial tip cells lead the way. Arteriosclerosis, Thrombosis, and Vascular Biology. 2009; 29: 639\u2013649.","DOI":"10.1161\/ATVBAHA.109.185165."},{"key":"ref104","doi-asserted-by":"publisher","unstructured":"Lobov IB, Renard RA, Papadopoulos N, Gale NW, Thurston G, Yancopoulos GD, et al. Delta-like ligand 4 (Dll4) is induced by VEGF as a negative regulator of angiogenic sprouting. Proceedings of the National Academy of Sciences of the United States of America. 2007; 104: 3219\u20133224.","DOI":"10.1073\/pnas.0611206104."},{"key":"ref105","doi-asserted-by":"publisher","unstructured":"Noguera-Troise I, Daly C, Papadopoulos NJ, Coetzee S, Boland P, Gale NW, et al. 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Neuroscience Letters. 1994; 177: 23\u201326.","DOI":"10.1016\/0304-3940(94)90035-3."},{"key":"ref112","doi-asserted-by":"publisher","unstructured":"Duong T, Acton PJ, Johnson RA. The in vitro neuronal toxicity of pentraxins associated with Alzheimer\u2019s disease brain lesions. Brain Research. 1998; 813: 303\u2013312.","DOI":"10.1016\/s0006-8993(98)00966-4."},{"key":"ref113","doi-asserted-by":"publisher","unstructured":"Engelhart MJ, Geerlings MI, Meijer J, Kiliaan A, Ruitenberg A, van Swieten JC, et al. Inflammatory proteins in plasma and the risk of dementia: the rotterdam study. Archives of Neurology. 2004; 61: 668\u2013672.","DOI":"10.1001\/archneur.61.5.668."},{"key":"ref114","doi-asserted-by":"publisher","unstructured":"Dik MG, Jonker C, Hack CE, Smit JH, Comijs HC, Eikelenboom P. Serum inflammatory proteins and cognitive decline in older persons. 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Cancer Research. 1993; 53: 3154\u20133158."},{"key":"ref121","doi-asserted-by":"publisher","unstructured":"Nahalkova J, Volkmann I, Aoki M, Winblad B, Bogdanovic N, Tjernberg LO, et al. CD147, a gamma-secretase associated protein is upregulated in Alzheimer\u2019s disease brain and its cellular trafficking is affected by presenilin-2. Neurochemistry International. 2010; 56: 67\u201376.","DOI":"10.1016\/j.neuint.2009.09.003."},{"key":"ref122","doi-asserted-by":"publisher","unstructured":"Vetrivel KS, Zhang X, Meckler X, Cheng H, Lee S, Gong P, et al. Evidence that CD147 modulation of beta-amyloid (Abeta) levels is mediated by extracellular degradation of secreted Abeta. The Journal of Biological Chemistry. 2008; 283: 19489\u201319498.","DOI":"10.1074\/jbc.M801037200."},{"key":"ref123","doi-asserted-by":"publisher","unstructured":"Cui D, Yamamoto K, Ikeda E. High-Mannose-Type Glycan of Basigin in Endothelial Cells Is Essential for the Opening of the Blood-Brain Barrier Induced by Hypoxia, Cyclophilin A, or Tumor Necrosis Factor-\u03b1. The American Journal of Pathology. 2024; 194: 612\u2013625.","DOI":"10.1016\/j.ajpath.2023.11.007."},{"key":"ref124","doi-asserted-by":"publisher","unstructured":"Muramatsu T. Basigin (CD147), a multifunctional transmembrane glycoprotein with various binding partners. Journal of Biochemistry. 2016; 159: 481\u2013490.","DOI":"10.1093\/jb\/mvv127."},{"key":"ref125","doi-asserted-by":"publisher","unstructured":"Washington AV, Schubert RL, Quigley L, Disipio T, Feltz R, Cho EH, et al. A TREM family member, TLT-1, is found exclusively in the alpha-granules of megakaryocytes and platelets. Blood. 2004; 104: 1042\u20131047.","DOI":"10.1182\/blood-2004-01-0315."},{"key":"ref126","doi-asserted-by":"crossref","unstructured":"Giomarelli B, Washington VA, Chisholm MM, Quigley L, McMahon JB, Mori T, et al. Inhibition of thrombin-induced platelet aggregation using human single-chain Fv antibodies specific for TREM-like transcript-1. Thrombosis and Haemostasis. 2007; 97: 955\u2013963.","DOI":"10.1160\/TH06-08-0456"},{"key":"ref127","doi-asserted-by":"publisher","unstructured":"Carrasquillo MM, Allen M, Burgess JD, Wang X, Strickland SL, Aryal S, et al. A candidate regulatory variant at the TREM gene cluster associates with decreased Alzheimer\u2019s disease risk and increased TREML1 and TREM2 brain gene expression. Alzheimer\u2019s & Dementia. 2017; 13: 663\u2013673.","DOI":"10.1016\/j.jalz.2016.10.005."},{"key":"ref128","doi-asserted-by":"publisher","unstructured":"Washington AV. Abstract 535: Trem Like Transcript-1 (TLT-1) Regulates Both Leukocytes and Endothelial Cells to Mediate Immunohemostasis. Arteriosclerosis, Thrombosis, and Vascular Biology. 2016; 36: A535\u2013A535.","DOI":"10.1161\/atvb.36.suppl_1.535."},{"key":"ref129","doi-asserted-by":"publisher","unstructured":"Schmoker AM, Perez Pearson LM, Cruz C, Colon Flores LG, Branfeild S, Pag\u00e1n Torres FD, et al. Defining the TLT-1 interactome from resting and activated human platelets. Journal of Proteomics. 2020; 215: 103638.","DOI":"10.1016\/j.jprot.2020.103638."},{"key":"ref130","doi-asserted-by":"publisher","unstructured":"Kniewallner KM, Wenzel D, Humpel C. Thiazine Red(+) platelet inclusions in Cerebral Blood Vessels are first signs in an Alzheimer\u2019s Disease mouse model. Scientific Reports. 2016; 6: 28447.","DOI":"10.1038\/srep28447."},{"key":"ref131","doi-asserted-by":"publisher","unstructured":"Humpel C. Platelets: Their Potential Contribution to the Generation of Beta-amyloid Plaques in Alzheimer\u2019s Disease. 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