{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T09:58:33Z","timestamp":1765015113293,"version":"3.46.0"},"reference-count":30,"publisher":"IMR Press","issue":"11","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Province Natural Science Foundation","award":["LTGY23H050003","LTGY24H050004"],"award-info":[{"award-number":["LTGY23H050003","LTGY24H050004"]}]},{"name":"Wenzhou Committee of Science and Technology of China","award":["Y2023065"],"award-info":[{"award-number":["Y2023065"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: Peritoneal fibrosis is a significant complication arising from long-term peritoneal dialysis (PD), primarily due to the loss of peritoneal mesothelial cells (PMCs). Recent studies have implicated periostin (POSTN) in the progression of various fibrotic diseases; however, its specific role in PD-induced peritoneal fibrosis remains unclear. Sodium alginate (SA) microgels have emerged as promising carriers for cell encapsulation in tissue engineering and regenerative medicine. This study investigated the therapeutic potential of PMCs encapsulated in SA microgels (SA\/PMC) for reducing PD-induced peritoneal fibrosis, with a focus on the modulation by the periostin\/nuclear factor kappa-B (NF-\u03baB)\/CXCL8 signaling pathway. Methods: Primary human peritoneal mesothelial cells (PHPMCs) were isolated from the PD effluent of patients. The effect of SA encapsulation on PMCs proliferation was evaluated using a Cell Counting Kit 8 (CCK-8) assay. The expression levels of POSTN, NF-\u03baB p65, and CXCL8, as well as fibrosis markers, including \u03b1-smooth muscle actin (\u03b1-SMA), collagen I, transforming growth factor-\u03b2 (TGF-\u03b2), and fibronectin, were evaluated in patients undergoing PD and a PD mouse model. Results: Patients undergoing PD for 1 year exhibited elevated levels of POSTN, NF-\u03baB p65, CXCL8, and fibrosis markers compared with those undergoing PD for 1 week. Conclusions: Consistent results from in vivo and in vitro models demonstrated that PD and hyperglycemic conditions upregulated the expression of POSTN, NF-\u03baB p65, CXCL8, and profibrotic markers, leading to peritoneal thickening and fibrotic progression. Treatment with SA\/PMC microgels ameliorated these effects. By modulating the POSTN\/NF-\u03baB\/CXCL8 pathway and enhancing PMCs survival, SA\/PMC microgels may have therapeutic potential in mitigating peritoneal fibrosis in PD patients.<\/jats:p>","DOI":"10.31083\/fbl45614","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T09:55:11Z","timestamp":1765014911000},"source":"Crossref","is-referenced-by-count":0,"title":["Sodium Alginate Microgel-Wrapped Primary Human Peritoneal Mesothelial Cells for the Treatment of Peritoneal Fibrosis Caused by Dialysis via the POSTN\/NF-\u03baB\/CXCL8 Pathway"],"prefix":"10.31083","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9581-8216","authenticated-orcid":true,"given":"Shuqing","family":"Ma","sequence":"first","affiliation":[{"name":"Zhejiang Key Laboratory of Intelligent Cancer Biomarker Discovery and Translation, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"},{"name":"The First School of Medicine and the School of Information and Engineering, Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyan","family":"Qiu","sequence":"additional","affiliation":[{"name":"The First School of Medicine and the School of Information and Engineering, Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jue","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First School of Medicine and the School of Information and Engineering, Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luhui","family":"Wang","sequence":"additional","affiliation":[{"name":"The First School of Medicine and the School of Information and Engineering, Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaozhe","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition, Wenzhou People\u2019s Hospital & Wenzhou Women\u2019s and Children\u2019s Hospital, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3248-8279","authenticated-orcid":true,"given":"Ke","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First School of Medicine and the School of Information and Engineering, Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Wenzhou Key Laboratory of Biophysics, Wenzhou Institute, University of Chinese Academy of Sciences, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changcan","family":"Shi","sequence":"additional","affiliation":[{"name":"Wenzhou Key Laboratory of Biophysics, Wenzhou Institute, University of Chinese Academy of Sciences, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX 77001, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7154-7526","authenticated-orcid":true,"given":"Chenfei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaosheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3910-4731","authenticated-orcid":true,"given":"Yongheng","family":"Bai","sequence":"additional","affiliation":[{"name":"Zhejiang Key Laboratory of Intelligent Cancer Biomarker Discovery and Translation, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangping","family":"Shentu","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunwu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Geriarics Center, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3336-5757","authenticated-orcid":true,"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, China"},{"name":"Geriarics Center, The First Affiliated Hospital of Wenzhou Medical University, 325035 Wenzhou, Zhejiang, 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