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(Landmark Ed)"],"abstract":"<jats:p>Background: Temozolomide (TMZ) is a standard chemotherapeutic agent for glioma, but prolonged use frequently leads to drug resistance, reducing its therapeutic efficacy. Schisandrin B (Sch B), a lignan isolated from Schisandra chinensis, demonstrates promising anti-neoplastic activity. This study investigated the synergistic effects of Sch B and TMZ on U87 glioma cells to explore their combined influence on cell viability, apoptosis, and mitochondrial function. Methods: U87 glioma cells were treated with Sch B, TMZ, or their combination. Cell viability was assessed using MTT assays. Apoptosis was evaluated by Hoechst staining and flow cytometry, while JC-1 staining and Western blotting were used to assess mitochondrial membrane potential, oxidative stress markers, and apoptosis-related proteins. Cell cycle analysis and pre-treatment with Z-VAD-FMK were performed to confirm pathway involvement. Results: Combination treatment significantly reduced cell viability (54.14%) compared to TMZ (72.47%) or Sch B (70.4%) alone. Flow cytometry indicated elevated apoptosis (22.3%) in the combination group. JC-1 staining and protein expression analyses revealed mitochondrial depolarization, cytochrome c release, activation of caspase-3 and -9, and a decreased Bcl-2\/Bax ratio. The combined treatment induced G2\/M cell cycle arrest via p53\/p21 activation and increased oxidative stress. Pre-treatment with Z-VAD-FMK partially reversed these effects, confirming caspase-dependent mitochondrial apoptosis. Conclusions: Sch B enhances TMZ-induced cytotoxicity in U87 glioma cells by promoting mitochondrial dysfunction, oxidative stress, and caspase-mediated apoptosis. These findings suggest that Sch B may serve as a promising adjuvant to improve the efficacy of TMZ-based glioma therapy, warranting further validation in resistant and in-vivo models.<\/jats:p>","DOI":"10.31083\/fbl46410","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T09:55:11Z","timestamp":1765014911000},"source":"Crossref","is-referenced-by-count":0,"title":["Combined Schisandrin B and Temozolomide Treatment Induces Mitochondrial Apoptosis in Glioma Cells"],"prefix":"10.31083","volume":"30","author":[{"given":"Zebo","family":"Tang","sequence":"first","affiliation":[{"name":"Institute of Neuroscience, Translational Medicine Institute, Xi\u2019an Jiaotong University Health Science Center, 132013 Xi\u2019an, Shaanxi, China"},{"name":"College of Basic Medical Sciences, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaoran","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangyu","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Basic Medical Sciences, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liu","family":"Han","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jilin Medical University, 132013 Jilin, Jilin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9630-4011","authenticated-orcid":true,"given":"Jianxin","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, Translational Medicine Institute, Xi\u2019an Jiaotong University Health Science Center, 132013 Xi\u2019an, Shaanxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"16242","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Hong X, Zhang J, Zou J, Ouyang J, Xiao B, Wang P, et al. 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