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(Landmark Ed)"],"abstract":"<jats:p>Background: Subarachnoid hemorrhage (SAH) represents a severe subtype of hemorrhagic stroke and is associated with unfavorable clinical outcomes. Physical exercise is an effective behavioral intervention that reduces the risk of stroke and preserves neurological function. Whole-body vibration (WBV) is a straightforward form of physical exercise that requires minimal motor skill proficiency. It has positive effects on neuromuscular performance and cardiovascular responses. Methods: The present study aims to investigate the potential protective effects of WBV on SAH-induced brain damage and neurological dysfunction in rats. WBV was administered using a vibration platform, with animals stimulation at 30 Hz for two sessions per day over a 30-day period. A modified endovascular perforation technique was employed to establish the in vivo SAH model. Results: WBV markedly decreased SAH-induced brain edema and inhibited levels of 8-hydroxy-2\u2032-deoxyguanosine (8-OHdG) and MitoSOX, two markers of oxidative stress. Immunostaining analyses demonstrated that WBV significantly attenuated microglial activation (at 24 and 72 h) and astrocytic activation (at 24 h) in the cortical region following SAH. Consistently, WBV markedly inhibited the expression of pro-inflammatory cytokines, including tumor necrosis factor-\u03b1 (TNF-\u03b1) and interleukin-1\u03b2 (IL-1\u03b2), in brain tissue and serum. WBV pretreatment significantly inhibited cortical neuronal apoptosis and downregulated caspase-1 activation at 24 h post-SAH. In addition, WBV activated Sirt3 following SAH, and its protective effects were partially prevented by the Sirt3 inhibitor 3-TYP. Conclusions: Our present data indicate that WBV is a clinically potent strategy that protects against the SAH-induced brain damage and neurological dysfunction by regulating Sirt3 and neuroinflammation.<\/jats:p>","DOI":"10.31083\/fbl47016","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T07:25:36Z","timestamp":1780125936000},"source":"Crossref","is-referenced-by-count":0,"title":["Whole Body Vibration Regulates Neuroinflammation via Sirt3 After Experimental Subarachnoid Hemorrhage"],"prefix":"10.31083","volume":"31","author":[{"given":"Jian-Meng","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Neurology, Shaanxi Provincial People\u2019s Hospital, 710068 Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shaanxi Provincial People\u2019s Hospital, 710068 Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shaanxi Provincial People\u2019s Hospital, 710068 Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shaanxi Provincial People\u2019s Hospital, 710068 Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Mei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shaanxi Provincial People\u2019s Hospital, 710068 Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Juan","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shaanxi Provincial People\u2019s Hospital, 710068 Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3800-5249","authenticated-orcid":true,"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shaanxi Provincial People\u2019s Hospital, 710068 Xi\u2019an, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Etminan N, Rinkel GJ. Unruptured intracranial aneurysms: development, rupture and preventive management. Nature Reviews. Neurology. 2016; 12: 699\u2013713.","DOI":"10.1038\/nrneurol.2016.150"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"\u0160e\u0161elja K, \u0160imuni\u0107 E, Sobo\u010danec S, Podgorski II, Pinteri\u0107 M, Had\u017eija MP, et al. SIRT3-Mediated Mitochondrial Regulation and Driver Tissues in Systemic Aging. Genes. 2025; 16: 1497.","DOI":"10.3390\/genes16121497"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Hong C, Wei Y, Wang Y, Lv G, Dong X, Huang X. The mechanism and therapeutic potential of SIRT3 in central nervous system diseases: a review. Frontiers in Pharmacology. 2025; 16: 1652296.","DOI":"10.3389\/fphar.2025.1652296"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Rehman AS, Kumar P, Parvez S. Dopamine-D2-agonist targets mitochondrial dysfunction via diminishing Drp1 mediated fission and normalizing PGC1-\u03b1\/SIRT3 pathways in a rodent model of Subarachnoid Haemorrhage. Neuroscience. 2025; 564: 60\u201378.","DOI":"10.1016\/j.neuroscience.2024.11.028"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Chen T, Wang Y, Wang YH, Hang CH. The Mfn1-\u03b2IIPKC Interaction Regulates Mitochondrial Dysfunction via Sirt3 Following Experimental Subarachnoid Hemorrhage. Translational Stroke Research. 2022; 13: 845\u2013857.","DOI":"10.1007\/s12975-022-00999-5"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Wu X, Luo J, Liu H, Cui W, Feng D, Qu Y. SIRT3 protects against early brain injury following subarachnoid hemorrhage via promoting mitochondrial fusion in an AMPK dependent manner. Chinese Neurosurgical Journal. 2020; 6: 1.","DOI":"10.1186\/s41016-019-0182-7"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Hafez S, Eid Z, Alabasi S, Darwiche Y, Channaoui S, Hess DC. Mechanisms of Preconditioning Exercise-Induced Neurovascular Protection in Stroke. Journal of Stroke. 2021; 23: 312\u2013326.","DOI":"10.5853\/jos.2020.03006"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Seidel H, Heide R. Long-term effects of whole-body vibration: a critical survey of the literature. International Archives of Occupational and Environmental Health. 1986; 58: 1\u201326.","DOI":"10.1007\/BF00378536"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Boerema AS, Heesterbeek M, Boersma SA, Schoemaker R, de Vries EFJ, van Heuvelen MJG, et al. Beneficial Effects of Whole Body Vibration on Brain Functions in Mice and Humans. Dose-response: a Publication of International Hormesis Society. 2018; 16: 1559325818811756.","DOI":"10.1177\/1559325818811756"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Kitamoto T, Saegusa R, Tashiro T, Sakurai T, Yokote K, Tokuyama T. Favorable Effects of 24-Week Whole-Body Vibration on Glycemic Control and Comprehensive Diabetes Therapy in Elderly Patients with Type 2 Diabetes. Diabetes Therapy: Research, Treatment and Education of Diabetes and Related Disorders. 2021; 12: 1751\u20131761.","DOI":"10.1007\/s13300-021-01068-0"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Verschueren SMP, Roelants M, Delecluse C, Swinnen S, Vanderschueren D, Boonen S. Effect of 6-month whole body vibration training on hip density, muscle strength, and postural control in postmenopausal women: a randomized controlled pilot study. Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research. 2004; 19: 352\u2013359.","DOI":"10.1359\/JBMR.0301245"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Zhao L, He LX, Huang SN, Gong LJ, Li L, Lv YY, et al. Protection of dopamine neurons by vibration training and up-regulation of brain-derived neurotrophic factor in a MPTP mouse model of Parkinson\u2019s disease. Physiological Research. 2014; 63: 649\u2013657.","DOI":"10.33549\/physiolres.932743"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Raval AP, Schatz M, Bhattacharya P, d\u2019Adesky N, Rundek T, Dietrich WD, et al. Whole Body Vibration Therapy after Ischemia Reduces Brain Damage in Reproductively Senescent Female Rats. 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European Journal of Physical and Rehabilitation Medicine. 2013; 49: 439\u2013450."},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"Tankisheva E, Bogaerts A, Boonen S, Feys H, Verschueren S. Effects of intensive whole-body vibration training on muscle strength and balance in adults with chronic stroke: a randomized controlled pilot study. Archives of Physical Medicine and Rehabilitation. 2014; 95: 439\u2013446.","DOI":"10.1016\/j.apmr.2013.09.009"},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"Lau RWK, Yip SP, Pang MYC. Whole-body vibration has no effect on neuromotor function and falls in chronic stroke. Medicine and Science in Sports and Exercise. 2012; 44: 1409\u20131418.","DOI":"10.1249\/MSS.0b013e31824e4f8c"},{"key":"ref33","doi-asserted-by":"publisher","unstructured":"de Oliveira Manoel AL, Macdonald RL. Neuroinflammation as a Target for Intervention in Subarachnoid Hemorrhage. Frontiers in Neurology. 2018; 9: 292.","DOI":"10.3389\/fneur.2018.00292"},{"key":"ref34","doi-asserted-by":"publisher","unstructured":"Kooijman E, Nijboer CH, van Velthoven CTJ, Mol W, Dijkhuizen RM, Kesecioglu J, et al. Long-term functional consequences and ongoing cerebral inflammation after subarachnoid hemorrhage in the rat. PloS One. 2014; 9: e90584.","DOI":"10.1371\/journal.pone.0090584"},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Atangana E, Schneider UC, Blecharz K, Magrini S, Wagner J, Nieminen-Kelh\u00e4 M, et al. Intravascular Inflammation Triggers Intracerebral Activated Microglia and Contributes to Secondary Brain Injury After Experimental Subarachnoid Hemorrhage (eSAH). Translational Stroke Research. 2017; 8: 144\u2013156.","DOI":"10.1007\/s12975-016-0485-3"},{"key":"ref36","doi-asserted-by":"publisher","unstructured":"Smith JA, Das A, Ray SK, Banik NL. Role of pro-inflammatory cytokines released from microglia in neurodegenerative diseases. Brain Research Bulletin. 2012; 87: 10\u201320.","DOI":"10.1016\/j.brainresbull.2011.10.004"},{"key":"ref37","doi-asserted-by":"publisher","unstructured":"Zeiler FA, Thelin EP, Czosnyka M, Hutchinson PJ, Menon DK, Helmy A. Cerebrospinal Fluid and Microdialysis Cytokines in Aneurysmal Subarachnoid Hemorrhage: A Scoping Systematic Review. Frontiers in Neurology. 2017; 8: 379.","DOI":"10.3389\/fneur.2017.00379"},{"key":"ref38","doi-asserted-by":"publisher","unstructured":"He X, Zeng H, Chen JX. Emerging role of SIRT3 in endothelial metabolism, angiogenesis, and cardiovascular disease. Journal of Cellular Physiology. 2019; 234: 2252\u20132265.","DOI":"10.1002\/jcp.27200"},{"key":"ref39","doi-asserted-by":"publisher","unstructured":"You Y, Wang Z. Roles of SIRT3 in aging and aging-related diseases. International Journal of Biological Sciences. 2025; 21: 5135\u20135163.","DOI":"10.7150\/ijbs.115518"},{"key":"ref40","doi-asserted-by":"publisher","unstructured":"Koentges C, Bode C, Bugger H. 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BioMed Research International. 2016; 2016: 8263926.","DOI":"10.1155\/2016\/8263926"},{"key":"ref44","doi-asserted-by":"publisher","unstructured":"Zhang K, Cheng H, Song L, Wei W. Inhibition of the Peroxisome Proliferator-Activated Receptor gamma Coactivator 1-alpha (PGC-1\u03b1)\/Sirtuin 3 (SIRT3) Pathway Aggravates Oxidative Stress After Experimental Subarachnoid Hemorrhage. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research. 2020; 26: e923688.","DOI":"10.12659\/MSM.923688"},{"key":"ref45","doi-asserted-by":"publisher","unstructured":"Sundaresan NR, Gupta M, Kim G, Rajamohan SB, Isbatan A, Gupta MP. Sirt3 blocks the cardiac hypertrophic response by augmenting Foxo3a-dependent antioxidant defense mechanisms in mice. The Journal of Clinical Investigation. 2009; 119: 2758\u20132771.","DOI":"10.1172\/JCI39162"},{"key":"ref46","doi-asserted-by":"publisher","unstructured":"Lanza IR, Short DK, Short KR, Raghavakaimal S, Basu R, Joyner MJ, et al. 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