{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:39:49Z","timestamp":1777700389910,"version":"3.51.4"},"reference-count":46,"publisher":"IMR Press","issue":"4","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013058","name":"Jiangsu Provincial Key Research and Development Program","doi-asserted-by":"crossref","award":["BE2022852"],"award-info":[{"award-number":["BE2022852"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Personnel Cultivation Program of Zhongda Hospital Southeast University","award":["CZXM-GSP-RC167"],"award-info":[{"award-number":["CZXM-GSP-RC167"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: Vascular calcification (VC) is an inflammatory disease driven by aberrant cellular processes in which macrophages play an important role. The A disintegrin and metalloproteinase 8 (ADAM8) protein is an important regulatory factor in macrophages and contributes to the development of inflammatory diseases. However, the relationship between ADAM8 and VC remains unknown. Thus, this study aimed to investigate the role of macrophage ADAM8 in VC. Methods: Plasma ADAM8 levels were compared between patients with and without aortic calcification. Immunofluorescence staining of human aortic tissue samples was performed to assess differences in ADAM8 expression between calcified and non-calcified tissues. Macrophage-specific Adam8 knockout mice (Adam8flox\/flox, Lyz2-Cre; KO) and the corresponding control mice (Adam8flox\/flox; Flox) were generated using CRISPR\/Cas9 technology. Additionally, the adeno-associated virus AAV6-F4\/80-Adam8 was employed to achieve macrophage-specific overexpression. The relationship between macrophage ADAM8 and VC was studied in a VC mouse model of chronic kidney disease (CKD) by comparing VC severity between groups. Results: Plasma ADAM8 levels were elevated in patients with aortic calcification. Immunofluorescence staining of human aortic samples suggested that VC was associated with ADAM8-positive macrophage infiltration and ADAM8 released by macrophages. In mice, macrophage-specific Adam8 knockout attenuated the development of CKD-associated VC, whereas macrophage-specific ADAM8 overexpression reversed the VC phenotype. Conclusion: ADAM8 levels are elevated in patients with aortic calcification and are associated with macrophage infiltration into vascular tissue and ADAM8 release, leading to increased VC. These findings identify ADAM8 as a promising novel therapeutic target for preventing VC.<\/jats:p>","DOI":"10.31083\/fbl49495","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:29:27Z","timestamp":1777454967000},"source":"Crossref","is-referenced-by-count":0,"title":["ADAM8 Deficiency in Macrophages Alleviates Vascular Calcification in Chronic Kidney Disease"],"prefix":"10.31083","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6396-2229","authenticated-orcid":true,"given":"Rong","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"},{"name":"Department of Cardiology, Yancheng No. 1 People\u2019s Hospital, Affiliated Hospital of Medical School, Nanjing University, 224000 Yancheng, Jiangsu, China"},{"name":"Department of Cardiology, The First people\u2019s Hospital of Yancheng, 224000 Yancheng, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0299-8527","authenticated-orcid":true,"given":"Zhenjun","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4833-6122","authenticated-orcid":true,"given":"Mi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2633-2316","authenticated-orcid":true,"given":"Zaixiao","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3410-7481","authenticated-orcid":true,"given":"Xinxin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8655-4017","authenticated-orcid":true,"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3705-2037","authenticated-orcid":true,"given":"Junwei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"},{"name":"Department of Cardiology, Nanjing Chest Hospital, Affiliated Nanjing Brain Hospital, Nanjing Medical University, 210029 Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4332-3013","authenticated-orcid":true,"given":"Genshan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Zhongda Hospital, 210009 Nanjing, Jiangsu, China"},{"name":"School of Medicine, Southeast University, 210009 Nanjing, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"16242","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Xu C, Smith ER, Tiong MK, Ruderman I, Toussaint ND. Interventions To Attenuate Vascular Calcification Progression in Chronic Kidney Disease: A Systematic Review of Clinical Trials. Journal of the American Society of Nephrology. 2022; 33: 1011\u20131032.","DOI":"10.1681\/ASN.2021101327"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Li X, Liu A, Xie C, Chen Y, Zeng K, Xie C, et al. The transcription factor GATA6 accelerates vascular smooth muscle cell senescence-related arterial calcification by counteracting the role of anti-aging factor SIRT6 and impeding DNA damage repair. Kidney International. 2024; 105: 115\u2013131.","DOI":"10.1016\/j.kint.2023.09.028"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Liu X, Chen A, Liang Q, Yang X, Dong Q, Fu M, et al. Spermidine inhibits vascular calcification in chronic kidney disease through modulation of SIRT1 signaling pathway. Aging Cell. 2021; 20: e13377.","DOI":"10.1111\/acel.13377"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Zheng G, Zhao Y, Li Z, Hua Y, Zhang J, Miao Y, et al. GLSP and GLSP-derived triterpenes attenuate atherosclerosis and aortic calcification by stimulating ABCA1\/G1-mediated macrophage cholesterol efflux and inactivating RUNX2-mediated VSMC osteogenesis. Theranostics. 2023; 13: 1325\u20131341.","DOI":"10.7150\/thno.80250"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Byun KA, Oh S, Yang JY, Lee SY, Son KH, Byun K. Ecklonia cava extracts decrease hypertension-related vascular calcification by modulating PGC-1\u03b1 and SOD2. Biomedicine & Pharmacotherapy. 2022; 153: 113283.","DOI":"10.1016\/j.biopha.2022.113283"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Lanzer P, Hannan FM, Lanzer JD, Janzen J, Raggi P, Furniss D, et al. Medial Arterial Calcification: JACC State-of-the-Art Review. Journal of the American College of Cardiology. 2021; 78: 1145\u20131165.","DOI":"10.1016\/j.jacc.2021.06.049"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Ma W, Jia K, Cheng H, Xu H, Li Z, Zhang H, et al. Orphan Nuclear Receptor NR4A3 Promotes Vascular Calcification via Histone Lactylation. Circulation Research. 2024; 134: 1427\u20131447.","DOI":"10.1161\/CIRCRESAHA.123.323699"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Bourne LE, Wheeler-Jones CP, Orriss IR. Regulation of mineralisation in bone and vascular tissue: a comparative review. The Journal of Endocrinology. 2021; 248: R51\u2013R65.","DOI":"10.1530\/JOE-20-0428"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Cannata-Andia JB, Roman-Garcia P, Hruska K. The connections between vascular calcification and bone health. Nephrology Dialysis Transplantation. 2011; 26: 3429\u20133436.","DOI":"10.1093\/ndt\/gfr591"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Jiang W, Zhang Z, Li Y, Chen C, Yang H, Lin Q, et al. The Cell Origin and Role of Osteoclastogenesis and Osteoblastogenesis in Vascular Calcification. Frontiers in Cardiovascular Medicine. 2021; 8: 639740.","DOI":"10.3389\/fcvm.2021.639740"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Zhai X, Cao S, Wang J, Qiao B, Liu X, Hua R, et al. Carbonylation of Runx2 at K176 by 4-Hydroxynonenal Accelerates Vascular Calcification. Circulation. 2024; 149: 1752\u20131769.","DOI":"10.1161\/CIRCULATIONAHA.123.065830"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Liu Y, Shanahan CM. Signalling pathways and vascular calcification. Frontiers in Bioscience (Landmark Edition). 2011; 16: 1302\u20131314.","DOI":"10.2741\/3790"},{"key":"ref13","unstructured":"Li XF, Wang Y, Zheng DD, Xu HX, Wang T, Pan M, et al. M1 macrophages promote aortic valve calcification mediated by microRNA-214\/TWIST1 pathway in valvular interstitial cells. American Journal of Translational Research. 2016; 8: 5773\u20135783."},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Ma WQ, Sun XJ, Zhu Y, Liu NF. PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. Cell Death & Disease. 2020; 11: 991.","DOI":"10.1038\/s41419-020-03162-w"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Ma WQ, Sun XJ, Zhu Y, Liu NF. Metformin attenuates hyperlipidaemia-associated vascular calcification through anti-ferroptotic effects. Free Radical Biology & Medicine. 2021; 165: 229\u2013242.","DOI":"10.1016\/j.freeradbiomed.2021.01.033"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Ye Y, Chen A, Li L, Liang Q, Wang S, Dong Q, et al. Repression of the antiporter SLC7A11\/glutathione\/glutathione peroxidase 4 axis drives ferroptosis of vascular smooth muscle cells to facilitate vascular calcification. Kidney International. 2022; 102: 1259\u20131275.","DOI":"10.1016\/j.kint.2022.07.034"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Wang PW, Pang Q, Zhou T, Song XY, Pan YJ, Jia LP, et al. Irisin alleviates vascular calcification by inhibiting VSMC osteoblastic transformation and mitochondria dysfunction via AMPK\/Drp1 signaling pathway in chronic kidney disease. Atherosclerosis. 2022; 346: 36\u201345.","DOI":"10.1016\/j.atherosclerosis.2022.02.007"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Rao Z, Zheng Y, Xu L, Wang Z, Zhou Y, Chen M, et al. Endoplasmic Reticulum Stress and Pathogenesis of Vascular Calcification. Frontiers in Cardiovascular Medicine. 2022; 9: 918056.","DOI":"10.3389\/fcvm.2022.918056"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Li Y, Sun Z, Zhang L, Yan J, Shao C, Jing L, et al. Role of Macrophages in the Progression and Regression of Vascular Calcification. Frontiers in Pharmacology. 2020; 11: 661.","DOI":"10.3389\/fphar.2020.00661"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Mierke CT. The versatile roles of ADAM8 in cancer cell migration, mechanics, and extracellular matrix remodeling. Frontiers in Cell and Developmental Biology. 2023; 11: 1130823.","DOI":"10.3389\/fcell.2023.1130823"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Schick D, Babendreyer A, Wozniak J, Awan T, Noels H, Liehn E, et al. Elevated expression of the metalloproteinase ADAM8 associates with vascular diseases in mice and humans. Atherosclerosis. 2019; 286: 163\u2013171.","DOI":"10.1016\/j.atherosclerosis.2019.03.008"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Ainola M, Li TF, Mandelin J, Hukkanen M, Choi SJ, Salo J, et al. Involvement of a disintegrin and a metalloproteinase 8 (ADAM8) in osteoclastogenesis and pathological bone destruction. Annals of the Rheumatic Diseases. 2009; 68: 427\u2013434.","DOI":"10.1136\/ard.2008.088260"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Ishizuka H, Garc\u00eda-Palacios V, Lu G, Subler MA, Zhang H, Boykin CS, et al. ADAM8 enhances osteoclast precursor fusion and osteoclast formation in vitro and in vivo. Journal of Bone and Mineral Research. 2011; 26: 169\u2013181.","DOI":"10.1002\/jbmr.199"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Ji Z, Guo J, Zhang R, Zuo W, Xu Y, Qu Y, et al. ADAM8 deficiency in macrophages promotes cardiac repair after myocardial infarction via ANXA2-mTOR-autophagy pathway. Journal of Advanced Research. 2025; 73: 483\u2013499.","DOI":"10.1016\/j.jare.2024.07.037"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Singh A, Tandon S, Tandon C. An update on vascular calcification and potential therapeutics. Molecular Biology Reports. 2021; 48: 887\u2013896.","DOI":"10.1007\/s11033-020-06086-y"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"Wu M, Rementer C, Giachelli CM. Vascular calcification: an update on mechanisms and challenges in treatment. Calcified Tissue International. 2013; 93: 365\u2013373.","DOI":"10.1007\/s00223-013-9712-z"},{"key":"ref27","doi-asserted-by":"publisher","unstructured":"Sun Z, Liu J, Sun J, Xu Z, Liu W, Mao N, et al. Decreased Regional Spontaneous Brain Activity and Cognitive Dysfunction in Patients with Coronary Heart Disease: a Resting-state Functional MRI Study. Academic Radiology. 2023; 30: 1081\u20131091.","DOI":"10.1016\/j.acra.2022.11.022"},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Gadde KM, Yin X, Goldberg RB, Orchard TJ, Schl\u00f6gl M, Dabelea D, et al. Coronary Artery Calcium and Cognitive Decline in the Diabetes Prevention Program Outcomes Study. Journal of the American Heart Association. 2023; 12: e029671.","DOI":"10.1161\/JAHA.123.029671"},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"Lee SY, Chao CT, Huang JW, Huang KC. Vascular Calcification as an Underrecognized Risk Factor for Frailty in 1783 Community-Dwelling Elderly Individuals. Journal of the American Heart Association. 2020; 9: e017308.","DOI":"10.1161\/JAHA.120.017308"},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Nimcharoen T, Aung WPP, Makeudom A, Sastraruji T, Khongkhunthian S, Sirinirund B, et al. Reduced ADAM8 levels upon non-surgical periodontal therapy in patients with chronic periodontitis. Archives of Oral Biology. 2019; 97: 137\u2013143.","DOI":"10.1016\/j.archoralbio.2018.10.021"},{"key":"ref31","unstructured":"Mandelin J, Li TF, Hukkanen MVJ, Liljestr\u00f6m M, Chen ZK, Santavirta S, et al. Increased expression of a novel osteoclast-stimulating factor, ADAM8, in interface tissue around loosened hip prostheses. The Journal of Rheumatology. 2003; 30: 2033\u20132038."},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"Conrad C, Yildiz D, Cleary SJ, Margraf A, Cook L, Schlomann U, et al. ADAM8 signaling drives neutrophil migration and ARDS severity. JCI Insight. 2022; 7: e149870.","DOI":"10.1172\/jci.insight.149870"},{"key":"ref33","doi-asserted-by":"publisher","unstructured":"Romagnoli M, Mineva ND, Polmear M, Conrad C, Srinivasan S, Loussouarn D, et al. ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis. EMBO Molecular Medicine. 2014; 6: 278\u2013294.","DOI":"10.1002\/emmm.201303373"},{"key":"ref34","doi-asserted-by":"publisher","unstructured":"Passos LSA, Lupieri A, Becker-Greene D, Aikawa E. Innate and adaptive immunity in cardiovascular calcification. Atherosclerosis. 2020; 306: 59\u201367.","DOI":"10.1016\/j.atherosclerosis.2020.02.016"},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Cochain C, Vafadarnejad E, Arampatzi P, Pelisek J, Winkels H, Ley K, et al. Single-Cell RNA-Seq Reveals the Transcriptional Landscape and Heterogeneity of Aortic Macrophages in Murine Atherosclerosis. Circulation Research. 2018; 122: 1661\u20131674.","DOI":"10.1161\/CIRCRESAHA.117.312509"},{"key":"ref36","doi-asserted-by":"publisher","unstructured":"Chen Y, Waqar AB, Nishijima K, Ning B, Kitajima S, Matsuhisa F, et al. Macrophage-derived MMP-9 enhances the progression of atherosclerotic lesions and vascular calcification in transgenic rabbits. Journal of Cellular and Molecular Medicine. 2020; 24: 4261\u20134274.","DOI":"10.1111\/jcmm.15087"},{"key":"ref37","doi-asserted-by":"publisher","unstructured":"Li Q, Zhang C, Shi J, Yang Y, Xing X, Wang Y, et al. High-Phosphate-Stimulated Macrophage-Derived Exosomes Promote Vascular Calcification via let-7b-5p\/TGFBR1 Axis in Chronic Kidney Disease. Cells. 2022; 12: 161.","DOI":"10.3390\/cells12010161"},{"key":"ref38","doi-asserted-by":"publisher","unstructured":"van der Vorst EPC, Donners MMPC. ADAM8 in the cardiovascular system: An innocent bystander with clinical use? Atherosclerosis. 2019; 286: 147\u2013149.","DOI":"10.1016\/j.atherosclerosis.2019.04.205"},{"key":"ref39","doi-asserted-by":"publisher","unstructured":"Theodorou K, van der Vorst EPC, Gijbels MJ, Wolfs IMJ, Jeurissen M, Theelen TL, et al. Whole body and hematopoietic ADAM8 deficiency does not influence advanced atherosclerotic lesion development, despite its association with human plaque progression. Scientific Reports. 2017; 7: 11670.","DOI":"10.1038\/s41598-017-10549-x"},{"key":"ref40","doi-asserted-by":"publisher","unstructured":"Zhang Y, Tian Z, Gerard D, Yao L, Shofer FS, Cs-Szabo G, et al. Elevated inflammatory gene expression in intervertebral disc tissues in mice with ADAM8 inactivated. Scientific Reports. 2021; 11: 1804.","DOI":"10.1038\/s41598-021-81495-y"},{"key":"ref41","doi-asserted-by":"publisher","unstructured":"Hobson S, Arefin S, Kublickiene K, Shiels PG, Stenvinkel P. Senescent Cells in Early Vascular Ageing and Bone Disease of Chronic Kidney Disease-A Novel Target for Treatment. Toxins. 2019; 11: 82.","DOI":"10.3390\/toxins11020082"},{"key":"ref42","doi-asserted-by":"publisher","unstructured":"Fang YP, Zhao Y, Huang JY, Yang X, Liu Y, Zhang XL. The functional role of cellular senescence during vascular calcification in chronic kidney disease. Frontiers in Endocrinology. 2024; 15: 1330942.","DOI":"10.3389\/fendo.2024.1330942"},{"key":"ref43","doi-asserted-by":"publisher","unstructured":"Kooman JP, Dekker MJ, Usvyat LA, Kotanko P, van der Sande FM, Schalkwijk CG, et al. Inflammation and premature aging in advanced chronic kidney disease. American Journal of Physiology. Renal Physiology. 2017; 313: F938\u2013F950.","DOI":"10.1152\/ajprenal.00256.2017"},{"key":"ref44","doi-asserted-by":"publisher","unstructured":"Zhang K, Zhang Y, Feng W, Chen R, Chen J, Touyz RM, et al. Interleukin-18 Enhances Vascular Calcification and Osteogenic Differentiation of Vascular Smooth Muscle Cells Through TRPM7 Activation. Arteriosclerosis, Thrombosis, and Vascular Biology. 2017; 37: 1933\u20131943.","DOI":"10.1161\/ATVBAHA.117.309161"},{"key":"ref45","doi-asserted-by":"publisher","unstructured":"Zhang Y, Zhang K, Zhang Y, Zhou L, Huang H, Wang J. IL-18 Mediates Vascular Calcification Induced by High-Fat Diet in Rats With Chronic Renal Failure. Frontiers in Cardiovascular Medicine. 2021; 8: 724233.","DOI":"10.3389\/fcvm.2021.724233"},{"key":"ref46","doi-asserted-by":"publisher","unstructured":"Roumeliotis S, Roumeliotis A, Dounousi E, Eleftheriadis T, Liakopoulos V. Biomarkers of vascular calcification in serum. Advances in Clinical Chemistry. 2020; 98: 91\u2013147.","DOI":"10.1016\/bs.acc.2020.02.004"}],"container-title":["Frontiers in Bioscience-Landmark"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/31\/4\/10.31083\/FBL49495","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:30:27Z","timestamp":1777455027000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/31\/4\/10.31083\/FBL49495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,22]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026]]}},"alternative-id":["S2768-6701(26)02085-X"],"URL":"https:\/\/doi.org\/10.31083\/fbl49495","relation":{},"ISSN":["2768-6701","2768-6698"],"issn-type":[{"value":"2768-6701","type":"print"},{"value":"2768-6698","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,22]]},"article-number":"49495"}}