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Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: Ergothioneine (EGT) has been reported to alleviate estrogen deficiency-induced osteoporosis, but whether this protection is accompanied by alterations in the bone microvasculature accompany this protection remain unknown. Therefore, this study aimed to examine the effects of EGT in ovariectomized (OVX) mice, with particular attention to type H vessels. Methods: A total of 18 female C57BL\/6J mice were randomly assigned to the sham, OVX, and OVX + EGT groups (n = 6 each). EGT was initiated 24 h after OVX and administered for 12 weeks. Femoral structure was evaluated using micro-computed tomography, hematoxylin and eosin staining, immunohistochemistry, and immunofluorescence. Meanwhile, serum assays were used to characterize bone turnover and oxidative stress. In addition, mouse bone-derived type H endothelial cells were exposed to EGT. The localization of organic cation transporter novel type 1 (OCTN1) was examined by immunofluorescence. Angiogenic activity was measured in Matrigel, while OCTN1 gene and protein expression were assessed by reverse-transcription quantitative polymerase chain reaction and Western blotting, respectively. Results: In OVX mice, EGT increased bone mineral density and improved trabecular microarchitecture. Serum superoxide dismutase activity and glutathione concentrations were higher after EGT administration, whereas advanced oxidation protein products and malondialdehyde levels were lower. Bone sections also showed a larger CD31+ vascular area, more osterix-positive (Osx+) osteogenic cells, and a closer spatial association between these signals. EGT increased OCTN1 expression in vitro and promoted angiogenesis. Conclusions: Early administration of EGT alleviated OVX-induced bone loss. This effect may be linked to reduced oxidative stress and enhanced angiogenesis related to type H vessels.<\/jats:p>","DOI":"10.31083\/fbl54657","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:21:51Z","timestamp":1790698911000},"source":"Crossref","is-referenced-by-count":0,"title":["Ergothioneine Attenuates Ovariectomy-Induced Bone Loss in Association With Reduced Oxidative Stress and Enhanced Type H Vessel-Related Angiogenesis"],"prefix":"10.31083","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2847-1166","authenticated-orcid":true,"given":"Xinping","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, China"},{"name":"First Clinical Medical College, Gannan Medical University, 341000 Ganzhou, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, China"},{"name":"First Clinical Medical College, Gannan Medical University, 341000 Ganzhou, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, China"},{"name":"First Clinical Medical College, Gannan Medical University, 341000 Ganzhou, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchao","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, China"},{"name":"First Clinical Medical College, Gannan Medical University, 341000 Ganzhou, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8369-4073","authenticated-orcid":true,"given":"Kai","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The First Affiliated Hospital of Gannan Medical University, 341000 Ganzhou, Jiangxi, China"},{"name":"First Clinical Medical College, Gannan Medical University, 341000 Ganzhou, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Reid IR, Bolland MJ. Management of postmenopausal osteoporosis. Endocrine Reviews. 2026; 47: 468\u2013495.","DOI":"10.1210\/endrev\/bnag006"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Eastell R, O'Neill TW, Hofbauer LC, Langdahl B, Reid IR, Gold DT, et al. Postmenopausal osteoporosis. Nature Reviews. Disease Primers. 2016; 2: 16069.","DOI":"10.1038\/nrdp.2016.69"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Wang L, Yu W, Yin X, Cui L, Tang S, Jiang N, et al. Prevalence of Osteoporosis and Fracture in China: The China Osteoporosis Prevalence Study. JAMA Network Open. 2021; 4: e2121106.","DOI":"10.1001\/jamanetworkopen.2021.21106"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Willers C, Norton N, Harvey NC, Jacobson T, Johansson H, Lorentzon M, et al. Osteoporosis in Europe: a compendium of country-specific reports. Archives of Osteoporosis. 2022; 17: 23.","DOI":"10.1007\/s11657-021-00969-8"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"GBD 2019 Fracture Collaborators. Global, regional, and national burden of bone fractures in 204 countries and territories, 1990-2019: a systematic analysis from the Global Burden of Disease Study 2019. The Lancet. Healthy Longevity. 2021; 2: e580\u2013e592.","DOI":"10.1016\/S2666-7568(21)00172-0"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Weitzmann MN, Pacifici R. Estrogen deficiency and bone loss: an inflammatory tale. The Journal of Clinical Investigation. 2006; 116: 1186\u20131194.","DOI":"10.1172\/JCI28550"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"Manolagas SC. From estrogen-centric to aging and oxidative stress: a revised perspective of the pathogenesis of osteoporosis. Endocrine Reviews. 2010; 31: 266\u2013300.","DOI":"10.1210\/er.2009-0024"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"Sies H. Oxidative Stress: Concept and Some Practical Aspects. Antioxidants (Basel, Switzerland). 2020; 9: 852.","DOI":"10.3390\/antiox9090852"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"Sies H, Jones DP. Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nature Reviews. Molecular Cell Biology. 2020; 21: 363\u2013383.","DOI":"10.1038\/s41580-020-0230-3"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 2007; 39: 44\u201384.","DOI":"10.1016\/j.biocel.2006.07.001"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"Marcucci G, Domazetovic V, Nediani C, Ruzzolini J, Favre C, Brandi ML. Oxidative Stress and Natural Antioxidants in Osteoporosis: Novel Preventive and Therapeutic Approaches. Antioxidants (Basel, Switzerland). 2023; 12: 373.","DOI":"10.3390\/antiox12020373"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"Vijayan V, Khandelwal M, Manglani K, Singh RR, Gupta S, Surolia A. Homocysteine alters the osteoprotegerin\/RANKL system in the osteoblast to promote bone loss: pivotal role of the redox regulator forkhead O1. Free Radical Biology & Medicine. 2013; 61: 72\u201384.","DOI":"10.1016\/j.freeradbiomed.2013.03.004"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Baek KH, Oh KW, Lee WY, Lee SS, Kim MK, Kwon HS, et al. Association of oxidative stress with postmenopausal osteoporosis and the effects of hydrogen peroxide on osteoclast formation in human bone marrow cell cultures. Calcified Tissue International. 2010; 87: 226\u2013235.","DOI":"10.1007\/s00223-010-9393-9"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Bai XC, Lu D, Bai J, Zheng H, Ke ZY, Li XM, et al. Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB. Biochemical and Biophysical Research Communications. 2004; 314: 197\u2013207.","DOI":"10.1016\/j.bbrc.2003.12.073"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Ran D, Zhou D, Liu G, Ma Y, Ali W, Yu R, et al. Reactive Oxygen Species Control Osteoblast Apoptosis through SIRT1\/PGC-1\u03b1\/P53Lys382 Signaling, Mediating the Onset of Cd-Induced Osteoporosis. Journal of Agricultural and Food Chemistry. 2023; 71: 5991\u20136002.","DOI":"10.1021\/acs.jafc.2c08505"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Jilka RL, Weinstein RS, Parfitt AM, Manolagas SC. Quantifying osteoblast and osteocyte apoptosis: challenges and rewards. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research. 2007; 22: 1492\u20131501.","DOI":"10.1359\/jbmr.070518"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Peng Y, Wu S, Li Y, Crane JL. Type H blood vessels in bone modeling and remodeling. Theranostics. 2020; 10: 426\u2013436.","DOI":"10.7150\/thno.34126"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Kusumbe AP, Ramasamy SK, Adams RH. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature. 2014; 507: 323\u2013328.","DOI":"10.1038\/nature13145"},{"key":"ref19","doi-asserted-by":"publisher","unstructured":"Zhao K, Zhu GZ, Li HZ, Gao JW, Tu C, Wu DZ, et al. Accumulation of Advanced Oxidation Protein Products Promotes Age-Related Decline of Type H Vessels in Bone. The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences. 2024; 80: glae271.","DOI":"10.1093\/gerona\/glae271"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Kim D, Piao J, Park JS, Lee D, Hwang DY, Hong HS. Substance P-Mediated Vascular Protection Ameliorates Bone Loss. Oxidative Medicine and Cellular Longevity. 2023; 2023: 9903336.","DOI":"10.1155\/2023\/9903336"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Liu HM, Tang W, Wang XY, Jiang JJ, Zhang W, Wang W. Safe and Effective Antioxidant: The Biological Mechanism and Potential Pathways of Ergothioneine in the Skin. Molecules (Basel, Switzerland). 2023; 28: 1648.","DOI":"10.3390\/molecules28041648"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"Apparoo Y, Phan CW, Kuppusamy UR, Sabaratnam V. Ergothioneine and its prospects as an anti-ageing compound. Experimental Gerontology. 2022; 170: 111982.","DOI":"10.1016\/j.exger.2022.111982"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Cumming BM, Chinta KC, Reddy VP, Steyn AJC. Role of Ergothioneine in Microbial Physiology and Pathogenesis. Antioxidants & Redox Signaling. 2018; 28: 431\u2013444.","DOI":"10.1089\/ars.2017.7300"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Paul BD. Ergothioneine: A Stress Vitamin with Antiaging, Vascular, and Neuroprotective Roles? Antioxidants & Redox Signaling. 2022; 36: 1306\u20131317.","DOI":"10.1089\/ars.2021.0043"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"Borodina I, Kenny LC, McCarthy CM, Paramasivan K, Pretorius E, Roberts TJ, et al. The biology of ergothioneine, an antioxidant nutraceutical. Nutrition Research Reviews. 2020; 33: 190\u2013217.","DOI":"10.1017\/S0954422419000301"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"Guo Z, Huang Y, Wang X, Han Y, Li A, Qu Y, et al. Ergothioneine alleviates osteoporosis via the ROS-MAPK signaling Axis. Bone. 2025; 197: 117496.","DOI":"10.1016\/j.bone.2025.117496"},{"key":"ref27","doi-asserted-by":"publisher","unstructured":"Li G, Fang Q, Lin Z, Zhang C, Gao C, Zhu D, et al. Ergothioneine prevents ovariectomy-induced osteoporosis by suppressing NF-\u03baB\/p65 signalling to attenuate osteoclastogenesis and bone marrow inflammation. Bone & Joint Research. 2026; 15: 248\u2013263.","DOI":"10.1302\/2046-3758.153.BJR-2025-0316.R2"},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Tang RMY, Cheah IKM, Yew TSK, Halliwell B. Distribution and accumulation of dietary ergothioneine and its metabolites in mouse tissues. Scientific Reports. 2018; 8: 1601.","DOI":"10.1038\/s41598-018-20021-z"},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"Batoon L, Millard SM, Raggatt LJ, Wu AC, Kaur S, Sun LWH, et al. Osteal macrophages support osteoclast-mediated resorption and contribute to bone pathology in a postmenopausal osteoporosis mouse model. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research. 2021; 36: 2214\u20132228.","DOI":"10.1002\/jbmr.4413"},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Luengo-Mateos M, Gonz\u00e1lez-Vila A, Torres Caldas AM, Alasaoufi AM, Gonz\u00e1lez-Dom\u00ednguez M, L\u00f3pez M, et al. Protocol for ovariectomy and estradiol replacement in mice. STAR Protocols. 2024; 5: 102910.","DOI":"10.1016\/j.xpro.2024.102910"},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"Martin KR. The bioactive agent ergothioneine, a key component of dietary mushrooms, inhibits monocyte binding to endothelial cells characteristic of early cardiovascular disease. Journal of Medicinal Food. 2010; 13: 1340\u20131346.","DOI":"10.1089\/jmf.2009.0194"},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"Chen JK, Deng YP, Jiang GJ, Liu YZ, Zhao T, Shen FM. Establishment of tube formation assay of bone marrow-derived endothelial progenitor cells. CNS Neuroscience & Therapeutics. 2013; 19: 533\u2013535.","DOI":"10.1111\/cns.12102"},{"key":"ref33","doi-asserted-by":"publisher","unstructured":"Lean JM, Davies JT, Fuller K, Jagger CJ, Kirstein B, Partington GA, et al. A crucial role for thiol antioxidants in estrogen-deficiency bone loss. The Journal of Clinical Investigation. 2003; 112: 915\u2013923.","DOI":"10.1172\/JCI18859"},{"key":"ref34","doi-asserted-by":"publisher","unstructured":"Mohamad NV, Ima-Nirwana S, Chin KY. Are Oxidative Stress and Inflammation Mediators of Bone Loss Due to Estrogen Deficiency? A Review of Current Evidence. Endocrine, Metabolic & Immune Disorders Drug Targets. 2020; 20: 1478\u20131487.","DOI":"10.2174\/1871530320666200604160614"},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Lean JM, Jagger CJ, Kirstein B, Fuller K, Chambers TJ. Hydrogen peroxide is essential for estrogen-deficiency bone loss and osteoclast formation. Endocrinology. 2005; 146: 728\u2013735.","DOI":"10.1210\/en.2004-1021"},{"key":"ref36","doi-asserted-by":"publisher","unstructured":"Huang J, Yin H, Rao SS, Xie PL, Cao X, Rao T, et al. Harmine enhances type H vessel formation and prevents bone loss in ovariectomized mice. Theranostics. 2018; 8: 2435\u20132446.","DOI":"10.7150\/thno.22144"},{"key":"ref37","doi-asserted-by":"publisher","unstructured":"Jia J, He R, Yao Z, Su J, Deng S, Chen K, et al. Daidzein alleviates osteoporosis by promoting osteogenesis and angiogenesis coupling. PeerJ. 2023; 11: e16121.","DOI":"10.7717\/peerj.16121"},{"key":"ref38","doi-asserted-by":"publisher","unstructured":"Sakata S, Kunimatsu R, Tanimoto K. Protective Effect of Ergothioneine against Oxidative Stress-Induced Chondrocyte Death. Antioxidants (Basel, Switzerland). 2024; 13: 800.","DOI":"10.3390\/antiox13070800"},{"key":"ref39","doi-asserted-by":"publisher","unstructured":"Raggatt LJ, Partridge NC. Cellular and molecular mechanisms of bone remodeling. The Journal of Biological Chemistry. 2010; 285: 25103\u201325108.","DOI":"10.1074\/jbc.R109.041087"},{"key":"ref40","doi-asserted-by":"publisher","unstructured":"Sivan U, De Angelis J, Kusumbe AP. Role of angiocrine signals in bone development, homeostasis and disease. Open Biology. 2019; 9: 190144.","DOI":"10.1098\/rsob.190144"},{"key":"ref41","doi-asserted-by":"publisher","unstructured":"Chen J, Hendriks M, Chatzis A, Ramasamy SK, Kusumbe AP. Bone Vasculature and Bone Marrow Vascular Niches in Health and Disease. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research. 2020; 35: 2103\u20132120.","DOI":"10.1002\/jbmr.4171"},{"key":"ref42","doi-asserted-by":"publisher","unstructured":"Long F, Li H, Chen X, He Y, Dong Y. Exosomal miR-423-5p Derived from Mineralized Osteoblasts Promotes Angiogenesis of Endothelial Cells by Targeting CXCL10. Frontiers in Bioscience (Landmark Edition). 2024; 29: 278.","DOI":"10.31083\/j.fbl2908278"},{"key":"ref43","doi-asserted-by":"publisher","unstructured":"Ribatti D, d'Amati A. Bone angiocrine factors. Frontiers in Cell and Developmental Biology. 2023; 11: 1244372.","DOI":"10.3389\/fcell.2023.1244372"},{"key":"ref44","doi-asserted-by":"publisher","unstructured":"Wille A, Weske S, von Wnuck Lipinski K, Wollnitzke P, Schr\u00f6der NH, Thomas N, et al. Sphingosine-1-phosphate promotes osteogenesis by stimulating osteoblast growth and neovascularization in a vascular endothelial growth factor-dependent manner. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research. 2024; 39: 357\u2013372.","DOI":"10.1093\/jbmr\/zjae006"},{"key":"ref45","doi-asserted-by":"publisher","unstructured":"Ramasamy SK, Kusumbe AP, Wang L, Adams RH. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature. 2014; 507: 376\u2013380.","DOI":"10.1038\/nature13146"},{"key":"ref46","doi-asserted-by":"publisher","unstructured":"Xu R, Yallowitz A, Qin A, Wu Z, Shin DY, Kim JM, et al. Targeting skeletal endothelium to ameliorate bone loss. Nature Medicine. 2018; 24: 823\u2013833.","DOI":"10.1038\/s41591-018-0020-z"},{"key":"ref47","doi-asserted-by":"publisher","unstructured":"Gr\u00fcndemann D, Harlfinger S, Golz S, Geerts A, Lazar A, Berkels R, et al. Discovery of the ergothioneine transporter. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102: 5256\u20135261.","DOI":"10.1073\/pnas.0408624102"},{"key":"ref48","doi-asserted-by":"publisher","unstructured":"Gr\u00fcndemann D, Hartmann L, Fl\u00f6gel S. The ergothioneine transporter (ETT): substrates and locations, an inventory. FEBS Letters. 2022; 596: 1252\u20131269.","DOI":"10.1002\/1873-3468.14269"},{"key":"ref49","doi-asserted-by":"publisher","unstructured":"Li RWS, Yang C, Sit ASM, Kwan YW, Lee SMY, Hoi MPM, et al. Uptake and protective effects of ergothioneine in human endothelial cells. The Journal of Pharmacology and Experimental Therapeutics. 2014; 350: 691\u2013700.","DOI":"10.1124\/jpet.114.214049"},{"key":"ref50","doi-asserted-by":"publisher","unstructured":"D'Onofrio N, Servillo L, Giovane A, Casale R, Vitiello M, Marfella R, et al. Ergothioneine oxidation in the protection against high-glucose induced endothelial senescence: Involvement of SIRT1 and SIRT6. Free Radical Biology & Medicine. 2016; 96: 211\u2013222.","DOI":"10.1016\/j.freeradbiomed.2016.04.013"}],"container-title":["Frontiers in Bioscience-Landmark"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/31\/9\/10.31083\/FBL54657","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:22:32Z","timestamp":1790698952000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/31\/9\/10.31083\/FBL54657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":50,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026]]}},"alternative-id":["S2768-6701(26)02269-0"],"URL":"https:\/\/doi.org\/10.31083\/fbl54657","relation":{},"ISSN":["2768-6701","2768-6698"],"issn-type":[{"value":"2768-6701","type":"print"},{"value":"2768-6698","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"article-number":"54657"}}