{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T02:14:37Z","timestamp":1784859277475,"version":"3.55.0"},"reference-count":32,"publisher":"IMR Press","issue":"7","license":[{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: Autosomal dominant polycystic kidney disease (ADPKD) is a ciliopathy characterized by abnormal tubular epithelial proliferation and fluid secretion. Anoctamin 1 (ANO1) is a calcium-dependent chloride channel. However, how ANO1 contributes to ADPKD is largely unexplored. Methods: Kidney tissues from ADPKD patients, Pkd1RC\/RC mice model, WT9-7 human PKD1+\u2063\/- cells, and 3D culture models in vitro were used. Localization of ANO1 and cilium length were investigated by confocal immunofluorescence. Results: We found that ANO1 was consistently upregulated in human and mouse PKD kidneys. Intriguingly, ANO1 located in a vesicle-like pattern at the ciliary base but not on the ciliary surface. ANO1 deficiency enhanced ciliogenesis and the ciliary dosage of polycystin-2 in human PKD cells, and reduced cyst formation in 3D culture models. Moreover, inhibition of ANO1 abolished the activation of STAT3 and ERK pathways in PKD cells. Conclusions: Our data indicate ANO1 is a negative regulator for both cilia length and cilia trafficking of polycystin-2 and provide mechanistic insights regarding the therapeutic potential of ANO1 pathway in ADPKD treatment.<\/jats:p>","DOI":"10.31083\/j.fbl2707216","type":"journal-article","created":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T09:25:48Z","timestamp":1657790748000},"source":"Crossref","is-referenced-by-count":6,"title":["Anoctamin 1 Inhibition Suppresses Cystogenesis by Enhancing Ciliogenesis and the Ciliary Dosage of Polycystins"],"prefix":"10.31083","volume":"27","author":[{"given":"Tao","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Nephrology, Shanghai Jiaotong University Medical School affiliated Shanghai Sixth People's Hospital, 200233 Shanghai, China"},{"name":"Division of Nephrology, Kidney Institution of Chinese People\u2019s Liberation Army, Changzheng Hospital, 200003 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihan","family":"Chen","sequence":"first","affiliation":[{"name":"Division of Nephrology, Kidney Institution of Chinese People\u2019s Liberation Army, Changzheng Hospital, 200003 Shanghai, China"},{"name":"Department of Nephrology and Rheumatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 200003 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingwen","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Xue","sequence":"first","affiliation":[{"name":"Division of Nephrology, Kidney Institution of Chinese People\u2019s Liberation Army, Changzheng Hospital, 200003 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Fu","sequence":"first","affiliation":[{"name":"Division of Nephrology, Kidney Institution of Chinese People\u2019s Liberation Army, Changzheng Hospital, 200003 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Ling","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghua","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55902, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changlin","family":"Mei","sequence":"first","affiliation":[{"name":"Division of Nephrology, Kidney Institution of Chinese People\u2019s Liberation Army, Changzheng Hospital, 200003 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"ref1","unstructured":"Laleye A, Awede B, Agboton B, Azonbakin S, Biaou O, Sagbo G, et al. 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