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Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: The cause of ulcerative colitis (UC) is not yet fully understood. Previous research has pointed towards a potential role for mutations in nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in promoting the onset and progression of inflammatory bowel disease (IBD) by altering the microbiota of the gut. However, the relationship between toll-like receptor 4 (TLR4) and gut microbiota in IBD is not well understood. To shed light on this, the interaction between TLR4 and gut microbiota was studied using a mouse model of IBD. Methods: To examine the function of TLR4 signaling in intestinal injury repair, researchers developed Dextran Sulfate Sodium Salt (DSS)-induced colitis and injury models in both wild-type (WT) mice and TLR4 knockout (TLR4-KO) mice. To assess changes in the gut microbiota, 16S rRNA sequencing was conducted on fecal samples from both the TLR4-KO and WT enteritis mouse models. Results: The data obtained depicted a protective function of TLR4 against DSS-induced colitis. The gut microbiota composition was found to vary considerably between the WT and TLR4-KO mice groups as indicated by \u03b2-diversity analysis and operational taxonomic units (OTUs) cluster. Statistical analysis of microbial multivariate variables depicted an elevated abundance of Escherichia coli\/Shigella, Gammaproteobacteria, Tenerlcutes, Deferribacteres, Enterobacteria, Rikenellaceae, and Proteobacteria in the gut microbiota of TLR4-KO mice, whereas there was a considerable reduction in Bacteroidetes at five different levels of the phylogenetic hierarchy including phylum, class, order, family, and genus in comparison with the WT control. Conclusions: TLR4 may protect intestinal epithelial cells from damage in response to DSS-induced injury by controlling the microbiota in the gut.<\/jats:p>","DOI":"10.31083\/j.fbl2808175","type":"journal-article","created":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T08:02:45Z","timestamp":1692950565000},"source":"Crossref","is-referenced-by-count":12,"title":["The Protective Role of TLR4 in Intestinal Epithelial Cells through the Regulation of the Gut Microbiota in DSS-Induced Colitis in Mice"],"prefix":"10.31083","volume":"28","author":[{"given":"Yun-Jie","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Anorectal Surgery, Changhai Hospital, Naval Medical University (Second Military Medical University), 200433 Shanghai, China"},{"name":"Department of Anorectal Surgery, Changzheng Hospital, Naval Medical University (Second Military Medical University), 200003 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Anorectal Surgery, Changhai Hospital, Naval Medical University (Second Military Medical University), 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pi-Qing","family":"Gong","sequence":"first","affiliation":[{"name":"Department of Anorectal Surgery, Changzheng Hospital, Naval Medical University (Second Military Medical University), 200003 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Radiation Medicine, Faculty of Naval Medicine, Naval Medical University (Second Military Medical University), 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Qian","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Anorectal Surgery, Changzheng Hospital, Naval Medical University (Second Military Medical University), 200003 Shanghai, China"},{"name":"Department of General Surgery, Tongji Hospital, School of Medicine, Tongji University, 200065 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anorectal Surgery, Changhai Hospital, Naval Medical University (Second Military Medical University), 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2023,8,23]]},"reference":[{"key":"ref1","unstructured":"Adams SM, Bornemann PH. 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