{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:49:43Z","timestamp":1771030183945,"version":"3.50.1"},"reference-count":56,"publisher":"IMR Press","issue":"6","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovak Agency for Research and Development","award":["APVV-18-0312"],"award-info":[{"award-number":["APVV-18-0312"]}]},{"name":"Slovak Agency for Research and Development","award":["APVV-21-0095"],"award-info":[{"award-number":["APVV-21-0095"]}]},{"name":"Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic and the Slovak Academy of Sciences","award":["VEGA 1\/0266\/20"],"award-info":[{"award-number":["VEGA 1\/0266\/20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: Apricot kernels containing amygdalin (AMG) as the major cyanogenic glycoside are potentially useful as a complementary therapy for the management of several ailments including cancer. Nevertheless, little is known regarding the toxic and therapeutic doses of AMG, particularly in terms of male reproduction. Hence, this study evaluates selected qualitative characteristics of rabbit testicular tissue following in vivo administration of AMG or apricot kernels for 28 days. Methods: The rabbits were randomly divided into five groups (Control, P1, P2, P3, P4). The Control received no AMG\/apricot kernels while the experimental groups P1 and P2 received a daily intramuscular injection of amygdalin at a dose of 0.6 and 3.0 mg\/kg of body weight (b.w.) for 28 days, respectively. P3 and P4 received a daily dose of 60 and 300 mg\/kg b.w. of crushed apricot kernels mixed with feed for 28 days, respectively. Changes to the testicular structure were quantified morphometrically, while tissue lysates were subjected to the evaluation of reactive oxygen species (ROS) production, total antioxidant capacity, activities of antioxidant enzymes, and glutathione concentration. The extent of damage to the proteins and lipids was quantified as well. Levels of selected cytokines were determined by the enzyme-linked immunosorbent assay while a luminometric approach was used to assess the activity of caspases. Results: Rabbits treated with 3.0 mg\/kg b.w. AMG presented a significantly increased protein oxidation (p = 0.0118) accompanied by a depletion of superoxide dismutase (p = 0.0464), catalase (p = 0.0317), and glutathione peroxidase (p = 0.0002). Significantly increased levels of interleukin-1 beta (p = 0.0012), tumor necrosis factors alpha (p = 0.0159), caspase-3\/7 (p = 0.0014), and caspase-9 (p = 0.0243) were also recorded in the experimental group P2 when compared to the Control. No effects were observed in the rabbits treated with apricot kernels at the oxidative, inflammatory, and histopathological levels. Conclusions: Apricot kernels did not induce toxicity in the testicular tissues of male rabbits, unlike pure AMG, which had a negative effect on male reproductive structures carried out through oxidative, inflammatory, and pro-apoptotic mechanisms.<\/jats:p>","DOI":"10.31083\/j.fbl2906235","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:52:57Z","timestamp":1719363177000},"source":"Crossref","is-referenced-by-count":2,"title":["The Effects of Apricot Kernels and Pure Amygdalin on the Structural, Oxidative, and Inflammatory Characteristics of Rabbit Testicular Tissue"],"prefix":"10.31083","volume":"29","author":[{"given":"Eva","family":"Tvrd\u00e1","sequence":"first","affiliation":[{"name":"Institute of Biotechnology, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"\u010eura\u010dka","sequence":"first","affiliation":[{"name":"AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Halen\u00e1r","sequence":"first","affiliation":[{"name":"Institute of Applied Biology, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juraj","family":"Pivko","sequence":"first","affiliation":[{"name":"Animal Production Research Centre Nitra, National Agricultural and Food Center, 951 41 Lu\u017eianky, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduard","family":"Koles\u00e1r","sequence":"first","affiliation":[{"name":"Institute of Applied Biology, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u013dubica","family":"Chrastinov\u00e1","sequence":"first","affiliation":[{"name":"Animal Production Research Centre Nitra, National Agricultural and Food Center, 951 41 Lu\u017eianky, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u013dubom\u00edr","family":"Ondru\u0161ka","sequence":"first","affiliation":[{"name":"Animal Production Research Centre Nitra, National Agricultural and Food Center, 951 41 Lu\u017eianky, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rastislav","family":"Jur\u010d\u00edk","sequence":"first","affiliation":[{"name":"Animal Production Research Centre Nitra, National Agricultural and Food Center, 951 41 Lu\u017eianky, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Koles\u00e1rov\u00e1","sequence":"first","affiliation":[{"name":"AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovakia"},{"name":"Institute of Applied Biology, Slovak University of Agriculture in Nitra, 949 76 Nitra, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"16242","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Siegel RL, Miller KD, Jemal A. Cancer Statistics, 2017. CA: a Cancer Journal for Clinicians. 2017; 67: 7\u201330.","DOI":"10.3322\/caac.21387"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Bidram E, Esmaeili Y, Ranji-Burachaloo H, Al-Zaubai N, Zarrabi A, Stewart A, et al. A concise review on cancer treatment methods and delivery systems. Journal of Drug Delivery Science and Technology. 2019; 54: 101350.","DOI":"10.1016\/j.jddst.2019.101350"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Lee SM, Choi HC, Hyun MK. An Overview of Systematic Reviews: Complementary Therapies for Cancer Patients. Integrative Cancer Therapies. 2019; 18: 1534735419890029.","DOI":"10.1177\/1534735419890029"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Duffy MJ. The war on cancer: are we winning? Tumour Biology. 2013; 34: 1275\u20131284.","DOI":"10.1007\/s13277-013-0759-2"},{"key":"ref5","unstructured":"World Health Organization. Cancer. Diagnosis and treatment. Available at: https:\/\/www.who.int\/activities\/ensuring-quality-treatment-for-cancer (Accessed: 31 October 2023)."},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Arthur K, Belliard JC, Hardin SB, Knecht K, Chen CS, Montgomery S. Practices, attitudes, and beliefs associated with complementary and alternative medicine (CAM) use among cancer patients. Integrative Cancer Therapies. 2012; 11: 232\u2013242.","DOI":"10.1177\/1534735411433832"},{"key":"ref7","unstructured":"World Health Organization. WHO Traditional Medicine Strategy: 2014\u20132023. 2013. Available at: https:\/\/www.who.int\/publications\/i\/item\/9789241506096 (Accessed: 31 October 2023)."},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Harris PE, Cooper KL, Relton C, Thomas KJ. Prevalence of complementary and alternative medicine (CAM) use by the general population: a systematic review and update. International Journal of Clinical Practice. 2012; 66: 924\u2013939.","DOI":"10.1111\/j.1742-1241.2012.02945.x"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Guerra-Mart\u00edn MD, Tejedor-Bueno MS, Correa-Casado M. Effectiveness of Complementary Therapies in Cancer Patients: A Systematic Review. International Journal of Environmental Research and Public Health. 2021; 18: 1017.","DOI":"10.3390\/ijerph18031017"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Barakat H, Aljutaily T, Almujaydil MS, Algheshairy RM, Alhomaid RM, Almutairi AS, et al. Amygdalin: A Review on Its Characteristics, Antioxidant Potential, Gastrointestinal Microbiota Intervention, Anticancer Therapeutic and Mechanisms, Toxicity, and Encapsulation. Biomolecules. 2022; 12: 1514.","DOI":"10.3390\/biom12101514"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Chaouali N, Gana I, Dorra A, Khelifi F, Nouioui A, Masri W, et al. Potential Toxic Levels of Cyanide in Almonds (Prunus amygdalus), Apricot Kernels (Prunus armeniaca), and Almond Syrup. ISRN Toxicology. 2013; 2013: 610648.","DOI":"10.1155\/2013\/610648"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Cassiem W, de Kock M. The anti-proliferative effect of apricot and peach kernel extracts on human colon cancer cells in vitro. BMC Complementary and Alternative Medicine. 2019; 19: 32.","DOI":"10.1186\/s12906-019-2437-4"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Contaminants in the Food Chain (CONTAM), Schrenk D, Bignami M, Bodin L, Chipman JK, Del Mazo J, et al. Evaluation of the health risks related to the presence of cyanogenic glycosides in foods other than raw apricot kernels. EFSA Journal. European Food Safety Authority. 2019; 17: e05662.","DOI":"10.2903\/j.efsa.2019.5662"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Chen Y, Ma J, Wang F, Hu J, Cui A, Wei C, et al. Amygdalin induces apoptosis in human cervical cancer cell line HeLa cells. Immunopharmacology and Immunotoxicology. 2013; 35: 43\u201351.","DOI":"10.3109\/08923973.2012.738688"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Makarevi\u0107 J, Rutz J, Juengel E, Kaulfuss S, Reiter M, Tsaur I, et al. Amygdalin blocks bladder cancer cell growth in vitro by diminishing cyclin A and cdk2. PLoS ONE. 2014; 9: e105590.","DOI":"10.1371\/journal.pone.0105590"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Chang HK, Shin MS, Yang HY, Lee JW, Kim YS, Lee MH, et al. Amygdalin induces apoptosis through regulation of Bax and Bcl-2 expressions in human DU145 and LNCaP prostate cancer cells. Biological & Pharmaceutical Bulletin. 2006; 29: 1597\u20131602.","DOI":"10.1248\/bpb.29.1597"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Saidu Y. Physicochemical features of rhodanese: A review. African Journal of Biotechnology. 2004; 3: 370\u2013374.","DOI":"10.5897\/AJB2004.000-2071"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Blaheta RA, Nelson K, Haferkamp A, Juengel E. Amygdalin, quackery or cure? Phytomedicine: International Journal of Phytotherapy and Phytopharmacology. 2016; 23: 367\u2013376.","DOI":"10.1016\/j.phymed.2016.02.004"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Kolesarova A, Pivko J, Halenar M, Zby\u0148ovsk\u00e1 K, Chrastinov\u00e1 \u013d, Ondru\u0161ka \u013d, et al. Effect of apricot seeds on renal structure of rabbits. Potravinarstvo\/Slovak Journal of Food Sciences. 2017; 11: 309\u2013314.","DOI":"10.5219\/751"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Halenar M, Chrastinova L, Ondruska L, Jurcik R, Zbynovska K, Tusimova E, et al. The evaluation of endocrine regulators after intramuscular and oral application of cyanogenic glycoside amygdalin in rabbits. Biologia. 2017; 72: 468\u2013474.","DOI":"10.1515\/biolog-2017-0044"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Kolesar E, Tvrda E, Halenar M, Schneidgenova M, Chrastinova L, Ondruska L, et al. Assessment of rabbit spermatozoa characteristics after amygdalin and apricot seeds exposure in vivo. Toxicology Reports. 2018; 5: 679\u2013686.","DOI":"10.1016\/j.toxrep.2018.05.015"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Kovacikova E, Kovacik A, Halenar M, Tokarova K, Chrastinova L, Ondruska L, et al. Potential toxicity of cyanogenic glycoside amygdalin and bitter apricot seed in rabbits-Health status evaluation. Journal of Animal Physiology and Animal Nutrition. 2019; 103: 695\u2013703.","DOI":"10.1111\/jpn.13055"},{"key":"ref23","unstructured":"Kovacova V, Sarocka A, Omelka R, Bauerova M, Grosskopf B, Formicki G, et al. Subacute exposure to amygdalin influences compact bone remodeling of rabbits. Journal of Physiology and Pharmacology. 2019; 70: 641\u2013648."},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Webb DR. Animal models of human disease: inflammation. Biochemical Pharmacology. 2014; 87: 121\u2013130.","DOI":"10.1016\/j.bcp.2013.06.014"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Esteves PJ, Abrantes J, Baldauf HM, BenMohamed L, Chen Y, Christensen N, et al. The wide utility of rabbits as models of human diseases. Experimental & Molecular Medicine. 2018; 50: 1\u201310.","DOI":"10.1038\/s12276-018-0094-1"},{"key":"ref26","unstructured":"Koles\u00e1r E. Determination of the effects of amygdalin on the rabbit male reproductive system. Dissertation Thesis. 2019. Available at: https:\/\/opac.crzp.sk\/?fn=*docview2&record=819BE1355532A4D3171B64AB1E91 (Accessed: 29 February 2024)."},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Tvrd\u00e1 E, Mass\u00e1nyi P, Luk\u00e1c N, Danko J, Schlarmannova J, Chrenek P. The effect of mammary gland-specific transgene expression on rabbit reproductive gland structure. Folia Biologica. 2014; 62: 119\u2013125.","DOI":"10.3409\/fb62_2.119"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"Tvrd\u00e1 E, Kov\u00e1\u010d J, Ferenczyov\u00e1 K, Kalo\u010dayov\u00e1 B, \u010eura\u010dka M, Benko F, et al. Quercetin Ameliorates Testicular Damage in Zucker Diabetic Fatty Rats through Its Antioxidant, Anti-Inflammatory and Anti-Apoptotic Properties. International Journal of Molecular Sciences. 2022; 23: 16056.","DOI":"10.3390\/ijms232416056"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Liu D, Li C, Chen Y, Burnett C, Liu XY, Downs S, et al. Nuclear import of proinflammatory transcription factors is required for massive liver apoptosis induced by bacterial lipopolysaccharide. The Journal of Biological Chemistry. 2004; 279: 48434\u201348442.","DOI":"10.1074\/jbc.M407190200"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Muller CH, Lee TKY, Monta\u00f1o MA. Improved chemiluminescence assay for measuring antioxidant capacity of seminal plasma. Methods in Molecular Biology. 2013; 927: 363\u2013376.","DOI":"10.1007\/978-1-62703-038-0_31"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Weber D, Davies MJ, Grune T. Determination of protein carbonyls in plasma, cell extracts, tissue homogenates, isolated proteins: Focus on sample preparation and derivatization conditions. Redox Biology. 2015; 5: 367\u2013380.","DOI":"10.1016\/j.redox.2015.06.005"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Beers RF, Jr, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. The Journal of Biological Chemistry. 1952; 195: 133\u2013140.","DOI":"10.1016\/S0021-9258(19)50881-X"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"Ellman GL. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics. 1959; 82: 70\u201377.","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"Mani J, Rutz J, Maxeiner S, Juengel E, Bon D, Roos F, et al. Cyanide and lactate levels in patients during chronic oral amygdalin intake followed by intravenous amygdalin administration. Complementary Therapies in Medicine. 2019; 43: 295\u2013299.","DOI":"10.1016\/j.ctim.2019.03.002"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"Savic IM, Nikolic VD, Savic-Gajic IM, Nikolic LB, Ibric SR, Gajic DG. Optimization of technological procedure for amygdalin isolation from plum seeds (Pruni domesticae semen). Frontiers in Plant Science. 2015; 6: 276.","DOI":"10.3389\/fpls.2015.00276"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"Akhone MA, Bains A, Tosif MM, Chawla P, Fogarasi M, Fogarasi S. Apricot Kernel: Bioactivity, Characterization, Applications, and Health Attributes. Foods. 2022; 11: 2184.","DOI":"10.3390\/foods11152184"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"Albogami S, Hassan A, Ahmed N, Alnefaie A, Alattas A, Alquthami L, et al. Evaluation of the effective dose of amygdalin for the improvement of antioxidant gene expression and suppression of oxidative damage in mice. PeerJ. 2020; 8: e9232.","DOI":"10.7717\/peerj.9232"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"Khandekar JD, Edelman H. Studies of amygdalin (laetrile) toxicity in rodents. JAMA. 1979; 242: 169\u2013171.","DOI":"10.1001\/jama.242.2.169"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"Shivanoor SM, Muniswamy D. Subchronic cyanide toxicity on male reproductive system of albino rat. Toxicology Research. 2015; 4: 57\u201364.","DOI":"10.1039\/C4TX00064A"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"Opyd PM, Jurgo\u0144ski A, Ju\u015bkiewicz J, Milala J, Zdu\u0144czyk Z, Kr\u00f3l B. Nutritional and Health-Related Effects of a Diet Containing Apple Seed Meal in Rats: The Case of Amygdalin. Nutrients. 2017; 9: 1091.","DOI":"10.3390\/nu9101091"},{"key":"ref41","doi-asserted-by":"crossref","unstructured":"Holzbecher MD, Moss MA, Ellenberger HA. The cyanide content of laetrile preparations, apricot, peach and apple seeds. Journal of Toxicology. Clinical Toxicology. 1984; 22: 341\u2013347.","DOI":"10.3109\/15563658408992565"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"Kamendulis LM, Zhang H, Wang Y, Klaunig JE. Morphological transformation and oxidative stress induced by cyanide in Syrian hamster embryo (SHE) cells. Toxicological Sciences. 2002; 68: 437\u2013443.","DOI":"10.1093\/toxsci\/68.2.437"},{"key":"ref43","doi-asserted-by":"crossref","unstructured":"Kadiri HE, Asagba SO. The chronic effects of cyanide on oxidative stress indices in the domestic chicken (Gallus domesticus L.). The Journal of Basic and Applied Zoology. 2019; 80: 30.","DOI":"10.1186\/s41936-019-0098-y"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"Dakin HD. The oxidation of amido-acids with the production of substances of biological importance. Journal of Biological Chemistry. 1906; 1: 171\u2013176.","DOI":"10.1016\/S0021-9258(17)46081-9"},{"key":"ref45","unstructured":"Aprioku JS. Pharmacology of free radicals and the impact of reactive oxygen species on the testis. Journal of Reproduction & Infertility. 2013; 14: 158\u2013172."},{"key":"ref46","doi-asserted-by":"crossref","unstructured":"Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity. 2008; 1: 15\u201324.","DOI":"10.4161\/oxim.1.1.6843"},{"key":"ref47","doi-asserted-by":"crossref","unstructured":"Erkkil\u00e4 K, Suomalainen L, Wikstr\u00f6m M, Parvinen M, Dunkel L. Chemical anoxia delays germ cell apoptosis in the human testis. Biology of Reproduction. 2003; 69: 617\u2013626.","DOI":"10.1095\/biolreprod.102.013920"},{"key":"ref48","doi-asserted-by":"crossref","unstructured":"Bernardi P, Scorrano L, Colonna R, Petronilli V, Di Lisa F. Mitochondria and cell death. Mechanistic aspects and methodological issues. European Journal of Biochemistry. 1999; 264: 687\u2013701.","DOI":"10.1046\/j.1432-1327.1999.00725.x"},{"key":"ref49","doi-asserted-by":"crossref","unstructured":"Vander Heiden MG, Chandel NS, Schumacker PT, Thompson CB. Bcl-xL prevents cell death following growth factor withdrawal by facilitating mitochondrial ATP\/ADP exchange. Molecular Cell. 1999; 3: 159\u2013167.","DOI":"10.1016\/S1097-2765(00)80307-X"},{"key":"ref50","doi-asserted-by":"crossref","unstructured":"Yang Y, Jiang G, Zhang P, Fan J. Programmed cell death and its role in inflammation. Military Medical Research. 2015; 2: 12.","DOI":"10.1186\/s40779-015-0039-0"},{"key":"ref51","doi-asserted-by":"crossref","unstructured":"Shou Y, Gunasekar PG, Borowitz JL, Isom GE. Cyanide-induced apoptosis involves oxidative-stress-activated NF-kappaB in cortical neurons. Toxicology and Applied Pharmacology. 2000; 164: 196\u2013205.","DOI":"10.1006\/taap.2000.8900"},{"key":"ref52","doi-asserted-by":"crossref","unstructured":"Attia H, Finocchi F, Orciani M, Mehdi M, Zidi Jrah I, Lazzarini R, et al. Pro-inflammatory cytokines and microRNAs in male infertility. Molecular Biology Reports. 2021; 48: 5935\u20135942.","DOI":"10.1007\/s11033-021-06593-6"},{"key":"ref53","doi-asserted-by":"crossref","unstructured":"Hasan H, Bhushan S, Fijak M, Meinhardt A. Mechanism of Inflammatory Associated Impairment of Sperm Function, Spermatogenesis and Steroidogenesis. Frontiers in Endocrinology. 2022; 13: 897029.","DOI":"10.3389\/fendo.2022.897029"},{"key":"ref54","doi-asserted-by":"crossref","unstructured":"Chojnacka K, Bilinska B, Mruk DD. Interleukin 1alpha-induced disruption of the Sertoli cell cytoskeleton affects gap junctional communication. Cellular Signalling. 2016; 28: 469\u2013480.","DOI":"10.1016\/j.cellsig.2016.02.003"},{"key":"ref55","doi-asserted-by":"crossref","unstructured":"Hamada A, Yoshioka S, Takuma D, Yokota J, Cui T, Kusunose M, et al. The effect of Eriobotrya japonica seed extract on oxidative stress in adriamycin-induced nephropathy in rats. Biological & Pharmaceutical Bulletin. 2004; 27: 1961\u20131964.","DOI":"10.1248\/bpb.27.1961"},{"key":"ref56","doi-asserted-by":"crossref","unstructured":"Felemban SG, Aldubayan MA, Alhowail AH, Almami IS. Vitamin B17 Ameliorates Methotrexate-Induced Reproductive Toxicity, Oxidative Stress, and Testicular Injury in Male Rats. Oxidative Medicine and Cellular Longevity. 2020; 2020: 4372719.","DOI":"10.1155\/2020\/4372719"}],"container-title":["Frontiers in Bioscience-Landmark"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/29\/6\/10.31083\/j.fbl2906235","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T14:04:47Z","timestamp":1732716287000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.imrpress.com\/journal\/FBL\/29\/6\/10.31083\/j.fbl2906235"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,25]]},"references-count":56,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024]]}},"alternative-id":["S2768-6701(24)01276-0"],"URL":"https:\/\/doi.org\/10.31083\/j.fbl2906235","relation":{},"ISSN":["2768-6701","2768-6698"],"issn-type":[{"value":"2768-6701","type":"print"},{"value":"2768-6698","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,25]]},"article-number":"235"}}