{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:59:42Z","timestamp":1787025582425,"version":"build-2736575974"},"reference-count":21,"publisher":"IMR Press","issue":"9","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Basic Research Development Program of China","award":["2015CB554000"],"award-info":[{"award-number":["2015CB554000"]}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82173005"],"award-info":[{"award-number":["82173005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81872559"],"award-info":[{"award-number":["81872559"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82103779"],"award-info":[{"award-number":["82103779"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Yun-Jie Shi's Second Outstanding Doctoral Start-up Grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Landmark Ed)"],"abstract":"<jats:p>Background: Ulcerative colitis (UC) is an intestinal disorder marked by chronic, recurring inflammation, yet its underlying mechanisms have not been fully elucidated. Methods: The current research dealt with examining the biological impacts of toll-like receptor 2 (TLR2) on dextran sulfate sodium (DSS)-triggered inflammation in the intestines of wild-type (WT) and TLR2-knockout (TLR2-KO) colitis mouse models. To elucidate the protective function of TLR2 in DSS-triggered colitis, RNA-sequencing (RNA-Seq) was carried out to compare the global gene expression data in the gut of WT and TLR2-KO mice. Further, 16S rRNA gene sequencing revealed notable variations in gut microbiota composition between WT and TLR2-KO colitis mice. Results: It was revealed that TLR2-KO mice exhibited increased susceptibility to DSS-triggered colitis. RNA-Seq results demonstrated that cell cycle pathway-related genes were notably downregulated in TLR2-KO colitis mice (enrichment score = 30, p &lt; 0.001). 16S rRNA gene sequencing revealed that in comparison to the WT colitis mice, the relative abundance of Marinifilacea (p = 0.006), Rikenellacea (p = 0.005), Desulfovibrionaceae (p = 0.045), Tannerellaceae (p = 0.038), Ruminococcaceae (p = 0.003), Clostridia (p = 0.027), and Mycoplasmataceae (p = 0.0009) was significantly increased at the family level in the gut of TLR2-KO colitis mice. In addition, microbiome diversity-transcriptome collaboration analysis highlighted that the relative abundance of Marinifilaceae was negatively linked to the expression of cell cycle signaling-related genes (p values were all less than 0.001). Conclusion: Based on these findings, we concluded that TLR2-KO exacerbates DSS-triggered intestinal injury by mitigating cell cycle signaling in a Marinifilaceae-dependent manner.<\/jats:p>","DOI":"10.31083\/j.fbl2909338","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T21:58:18Z","timestamp":1727301498000},"source":"Crossref","is-referenced-by-count":8,"title":["Toll-Like Receptor 2 Deficiency Exacerbates Dextran Sodium Sulfate-Induced Intestinal Injury through Marinifilaceae-Dependent Attenuation of Cell Cycle Signaling"],"prefix":"10.31083","volume":"29","author":[{"given":"Yun-Jie","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Wen","family":"Sheng","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Nan","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Radiology Intervention, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Radiation Medicine, Faculty of Naval Medicine, Naval Medical University, 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, 200433 Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Cho KK, Ko Y, Nilsen K, Verdon C, Femia G. 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