{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T21:21:28Z","timestamp":1771190488184,"version":"3.50.1"},"reference-count":32,"publisher":"IMR Press","issue":"3","license":[{"start":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:00:00Z","timestamp":1695513600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State assignment of the Ministry of Science and Higher Education","award":["FGFF-2022-0007"],"award-info":[{"award-number":["FGFF-2022-0007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Biosci. (Schol Ed)"],"abstract":"<jats:p>Introduction: Individual risk assessment of assisted reproductive technologies is essential for personalized treatment strategies. Genetic and genomic indicators of the response to stress by cells could provide individual prognostic indicators for in vitro fertilization (IVF) success. Such indicators include the copy number of ribosomal genes (rDNA), which modulates the level of protein synthesis, and the abundance of mitochondrial DNA (mtDNA), which provides the cell with energy, while the content of telomere repeats (TRs) indicate the biological age. Materials and Methods: The contents of the three repeats in DNA isolated from blood leukocytes of 40 women before and after ovarian stimulation were assayed prior to IVF. Then, we divided the women into a successful IVF group, IVF+ (N = 17, 7 cases of twins), and a group of failed cases, IVF\u2013 (N = 23). The control group included 17 non-pregnant women with natural childbirth in the past. The nonradioactive quantitative hybridization (NQH) method was applied to assay the genome repeat contents. Results: The number of rDNA copies in the IVF+ group was significantly higher than in the IVF\u2013 group (p &lt; 10-8). The number of mtDNA copies in the IVF+ group also exceeded those in the IVF\u2013 group (p &lt; 0.001), whereas the TR content in the two groups differed, albeit, non-significantly (p &lt; 0.03). Following the ovarian stimulation, the rDNA copy numbers did not change, while the contents of the mtDNA and TR varied significantly. Conclusions: This pilot study has shown that rDNA abundance in blood leukocytes can be considered a stable and effective predictor. Very low numbers of ribosomal repeat copies (&lt;330) entail a high risk of IVF failure. However, a combination of numerous mtDNA and TRs, provided that rDNA content is not very low, increases the probability of multiple pregnancies.<\/jats:p>","DOI":"10.31083\/j.fbs1503009","type":"journal-article","created":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T08:07:31Z","timestamp":1695542851000},"source":"Crossref","is-referenced-by-count":3,"title":["Ribosomal, Telomere, and Mitochondrial Repeat Copy Number Variations in Female Genomes during Ovarian Stimulation and the Prediction of In Vitro Fertilization Outcome: A Pilot Study"],"prefix":"10.31083","volume":"15","author":[{"given":"Natalia Nikolaevna","family":"Veiko","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizaveta Sergeevna","family":"Ershova","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3664-6685","authenticated-orcid":true,"given":"Lev Nikolaevich","family":"Porokhovnik","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Petrovna","family":"Klimenko","sequence":"first","affiliation":[{"name":"Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter Afanasievich","family":"Klimenko","sequence":"first","affiliation":[{"name":"Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel Evgenievich","family":"Umriukhin","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"},{"name":"Physiology Department, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0415dmund Viktorovich","family":"Kostyuk","sequence":"first","affiliation":[{"name":"Moscow Research and Practical Centre for Narcology of the Department of Public Health, 109390 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark Arkadievich","family":"Kurtser","sequence":"first","affiliation":[{"name":"Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana Nikolaevna","family":"Agafonova","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana Agaragimovna","family":"Salimova","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey Ivanovich","family":"Kutsev","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera Leonidovna","family":"Izhevskaya","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana Viktorovna","family":"Kostyuk","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Research Centre for Medical Genetics (RCMG), 115478 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"16242","published-online":{"date-parts":[[2023,9,24]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Czamanski-Cohen J, Sarid O, Cwikel J, Levitas E, Lunenfeld E, Douvdevani A, et al. 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