{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:47:13Z","timestamp":1787496433799,"version":"build-2736575974"},"reference-count":26,"publisher":"IMR Press","issue":"2","license":[{"start":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T00:00:00Z","timestamp":1708214400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Fund Project of Wannan Medical College","award":["WK2022XS45"],"award-info":[{"award-number":["WK2022XS45"]}]},{"name":"Collegiate Major Natural Science Research Projects of Anhui Province","award":["KJ2020A0603"],"award-info":[{"award-number":["KJ2020A0603"]}]},{"name":"Teaching and Research Project of Anhui Provincial Department of Education","award":["2019jxtd072"],"award-info":[{"award-number":["2019jxtd072"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Integr. Neurosci."],"abstract":"<jats:p>Background: Ischemic stroke is the most common form of stroke and the second most common cause of death and incapacity worldwide. Its pathogenesis and treatment have been the focus of considerable research. In traditional Chinese medicine, the root of Mongolian astragalus has been important in the treatment of stroke since ancient times. Astragalus polysaccharide (APS) is a key active ingredient of astragalus and offers therapeutic potential for conditions affecting the neurological system, the heart, cancer, and other disorders. However, it is not yet known how APS works to protect against ischemic stroke. Methods: Rats were subjected to middle cerebral artery occlusion (MCAO) to imitate localized cerebral ischemia. Each of four experimental groups (normal, sham, MCAO, and MCAO+APS) contained 12 adult male Sprague-Dawley (SD) rats selected randomly from a total of 48 rats. Following successful establishment of the model, rats in the MCAO+APS group received intraperitoneal injection of APS (50 mg\/kg) once daily for 14 days, whereas all other groups received no APS. The Bederson nerve function score and the forelimb placement test were used to detect motor and sensory function defects, while Nissl staining was used to investigate pathological defects in the ventroposterior thalamic nucleus (VPN). Immunohistochemical staining and Western blot were used to evaluate the expression of Neurogenic locus notch homolog protein 1 (Notch1), hairy and enhancer of split 1 (Hes1), phospho-nuclear factor-\u03baB p65 (p-NF\u03baB p65), and nuclear factor-\u03baB p65 (NF\u03baB p65) proteins in the VPN on the ischemic side of MCAO rats. Results: APS promoted the recovery of sensory and motor function, enhanced neuronal morphology, increased the number of neurons, and inhibited the expression of Notch1\/NF\u03baB signaling pathway proteins in the VPN of rats with cerebral ischemia. Conclusion: After cerebral ischemia, APS can alleviate symptoms of secondary damage to the VPN, which may be attributed to the suppression of the Notch1\/NF\u03baB pathway.<\/jats:p>","DOI":"10.31083\/j.jin2302034","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T03:19:44Z","timestamp":1708658384000},"source":"Crossref","is-referenced-by-count":8,"title":["Astragalus Polysaccharide Promotes Neuronal Injury Repair via the Notch1\/NF\u03baB Signaling Axis in the Ventroposterior Thalamic Nucleus in Rats with Focal Cerebral Ischemia"],"prefix":"10.31083","volume":"23","author":[{"given":"Wenjuan","family":"Li","sequence":"first","affiliation":[{"name":"School of basic medical sciences, Wannan Medical College, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huachun","family":"Miao","sequence":"first","affiliation":[{"name":"School of basic medical sciences, Wannan Medical College, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyin","family":"Nie","sequence":"first","affiliation":[{"name":"School of basic medical sciences, Wannan Medical College, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wu","sequence":"first","affiliation":[{"name":"School of basic medical sciences, Wannan Medical College, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaibin","family":"Li","sequence":"first","affiliation":[{"name":"School of basic medical sciences, Wannan Medical College, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2024,2,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Campbell BCV, De Silva DA, Macleod MR, Coutts SB, Schwamm LH, Davis SM, et al. 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