{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:27:50Z","timestamp":1765960070512,"version":"3.41.2"},"reference-count":36,"publisher":"IMR Press","issue":"3","license":[{"start":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T00:00:00Z","timestamp":1569801600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev. Cardiovasc. Med."],"abstract":"<jats:p>Cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy is a rare form of inherited cerebral small vessel disease associated with mutations in the high-temperature requirement serine peptidase A1 gene. As of now, only about 50 cases have been reported. In 2012, our group reported a family with a novel mutant of the high-temperature requirement serine peptidase A1 gene in China for the first time. To further explore the molecular pathogenesis of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy, a recombination mouse model expressed human high-temperature requirement serine peptidase A1 gene mutant identified by our group was generated using the Donor &amp; Clustered Regularly Interspaced Short Palindromic Repeats\/Cas9 system and termed the Mut-high-temperature requirement serine peptidase A1 geneL364P mouse model. Results show that Mut-high-temperature requirement serine peptidase A1 geneL364P mice present similar pathological characteristics to patients with cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy, suggesting that the Mut-high-temperature requirement serine peptidase A1 geneL364P mouse model was generated successfully. Moreover, apoptosis was induced in mouse brain vascular smooth muscle cells derived from Mut-high-temperature requirement serine peptidase A1 geneL364P mice. In summary, the cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy mouse model described in this study will be beneficial to demonstrate the pathological mechanism of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy and provide new therapeutic targets for clinical treatment.<\/jats:p>","DOI":"10.31083\/j.rcm.2019.03.31813","type":"journal-article","created":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T11:04:42Z","timestamp":1569841482000},"source":"Crossref","is-referenced-by-count":4,"title":["Establishment and identification of a novel HTRA1 mutation mice model"],"prefix":"10.31083","volume":"20","author":[{"given":"Chuanfen","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Resistance of Shandong Province, College of Life Science, Shandong Normal University, Jinan, 250000, P. R. China"},{"name":"Department of Neurology, General Hospital of Jinan Military Command of PLA, Jinan, 250000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Jin","sequence":"first","affiliation":[{"name":"College of physical education, Shandong normal university, 250000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurology, General Hospital of Jinan Military Command of PLA, Jinan, 250000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Li","sequence":"first","affiliation":[{"name":"Linyi Center for Disease Control and Prevention, Linyi, 276000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Resistance of Shandong Province, College of Life Science, Shandong Normal University, Jinan, 250000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingzhen","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Neurology, General Hospital of Jinan Military Command of PLA, Jinan, 250000, P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"16242","published-online":{"date-parts":[[2019,9,30]]},"reference":[{"key":"ref1","unstructured":"Arima, K., Yanagawa, S., Ito, N. and Ikeda, S. (2003) Cerebral arterial pathology of CADASIL and CARASIL (Maeda syndrome). Neuropathology 23, 327-334. be2b41135ee32f80a00d259e7c7d0802http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1046%2Fj.1440-1789.2003.00519.x%2Ffull"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"August, P. and Suthanthiran, M. (2006) Transforming growth factor beta signaling, vascular remodeling, and hypertension. The New England Journal of Medicine 354, 2721-2723.","DOI":"10.1056\/NEJMcibr062143"},{"key":"ref3","unstructured":"Bayrakli, F., Balaban, H., Gurelik, M., Hizmetli, S. and Topaktas, S. (2014) Mutation in the HTRA1 gene in a patient with degenerated spine as a component of CARASIL syndrome. Turkish neurosurgery 24, 67-69. 32e30870ef8a73c911adb5c6f363e8a0http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F24535794"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Bianchi, S., Di Palma, C., Gallus, G. N., Taglia, I., Poggiani, A., Rosini, F., Rufa, A., Muresanu, D. F., Cerase, A., Dotti, M. T. and Federico, A. (2014) Two novel HTRA1 mutations in a European CARASIL patient. Neurology 82, 898-900. 1e7a7cbe57bce2b5744a2dec088f46edhttp%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F24500651","DOI":"10.1212\/WNL.0000000000000202"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Chen, Y., He, Z., Meng, S., Li, L., Yang, H. and Zhang, X. (2013) A novel mutation of the high-temperature requirement A serine peptidase 1 (HTRA1) gene in a Chinese family with cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The Journal of International Medical Research 41, 1445-1455.","DOI":"10.1177\/0300060513480926"},{"key":"ref6","unstructured":"Chi, J., Meng, L., Pan, S., Lin, H., Zhai, X., Liu, L., Zhou, C., Jiang, C. and Guo, H. (2017) Primary Culture of Rat Aortic Vascular Smooth Muscle Cells: A New Method. International Medical Journal of Experimental and Clinical Research 23, 4014-4020. 423958e1466587e9a52211d49cad6af2http%3A%2F%2Fpubmedcentralcanada.ca%2Fpmcc%2Farticles%2FPMC5572779%2F"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Favaretto, S., Margoni, M., Salviati, L., Pianese, L., Manara, R. and Baracchini, C. (2019) A new Italian family with HTRA1 mutation associated with autosomal-dominant variant of CARASIL: Are we pointing towards a disease spectrum? Journal of the Neurological Sciences 396, 108-111.","DOI":"10.1016\/j.jns.2018.11.008"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Ferrari, G., Pintucci, G., Seghezzi, G., Hyman, K., Galloway, A. C. and Mignatti, P. (2006) VEGF, a prosurvival factor, acts in concert with TGF-beta1 to induce endothelial cell apoptosis. Proceedings of the National Academy of Sciences of the United States of America 103, 17260-17265. ddee9e2371c176cbf535ec8ebcdb8339http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F17088559","DOI":"10.1073\/pnas.0605556103"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Fukutake, T. (2011) Cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL): from discovery to gene identification. The Official Journal of National Stroke Association 20, 85-93. cbfee755f3ba7cd1fc301ccc9f60cafbhttp%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F21215656","DOI":"10.1016\/j.jstrokecerebrovasdis.2010.11.008"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Fukutake, T. and Hirayama, K. (1995) Familial young-adult-onset arteriosclerotic leukoencephalopathy with alopecia and lumbago without arterial hypertension. European Neurology 35, 69-79. 75665a4039e297739ad3ff36db572a46http%3A%2F%2Fwww.europepmc.org%2Fabstract%2FMED%2F7796840","DOI":"10.1159\/000117096"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Grainger, D. J. (2004) Transforming growth factor beta and atherosclerosis: so far, so good for the protective cytokine hypothesis. Arteriosclerosis, Thrombosis, and Vascular Biology 24, 399-404. 143422fbf325f8083d2ac99491f3059dhttp%3A%2F%2Feuropepmc.org%2Fabstract%2Fmed%2F14699019","DOI":"10.1161\/01.ATV.0000114567.76772.33"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Hara, K., Shiga, A., Fukutake, T., Nozaki, H., Miyashita, A., Yokoseki, A., Kawata, H., Koyama, A., Arima, K., Takahashi, T., Ikeda, M., Shiota, H., Tamura, M., Shimoe, Y., Hirayama, M., Arisato, T., Yanagawa, S., Tanaka, A., Nakano, I., Ikeda, S., Yoshida, Y., Yamamoto, T., Ikeuchi, T., Kuwano, R., Nishizawa, M., Tsuji, S. and Onodera, O. (2009) Association of HTRA1 mutations and familial ischemic cerebral small-vessel disease. The New England Journal of Medicine 360, 1729-1739. 2d18d6f0ac398449c0e30ccc75d41bc0http%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F19387015","DOI":"10.1056\/NEJMoa0801560"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Huang, R., Merrilees, M. J., Braun, K., Beaumont, B., Lemire, J., Clowes, A. W., Hinek, A. and Wight, T. N. (2006) Inhibition of versican synthesis by antisense alters smooth muscle cell phenotype and induces elastic fiber formation in vitro and in neointima after vessel injury. Circulation Research 98, 370-377. 881af7b2874c26e207c5f28e8e59f8d1http%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F16385080","DOI":"10.1161\/01.RES.0000202051.28319.c8"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Kenagy, R. D., Plaas, A. H. and Wight, T. N. (2006) Versican degradation and vascular disease. Trends in Cardiovascular Medicine 16, 209-215. 0e0e5079fb7082c90d975dcf85f6e663http%3A%2F%2Feuropepmc.org%2Farticles%2FPMC3169384","DOI":"10.1016\/j.tcm.2006.03.011"},{"key":"ref15","unstructured":"Lisignoli, G., Grassi, F., Zini, N., Toneguzzi, S., Piacentini, A., Guidolin, D., Bevilacqua, C. and Facchini, A. (2001) Anti-Fas-induced apoptosis in chondrocytes reduced by hyaluronan: evidence for CD44 and CD54 (intercellular adhesion molecule 1) invovement. Arthritis and rheumatism 44, 1800-1807. 0d8857c1f74f6b382614638d5d76f99ehttp%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1002%2F1529-0131%28200108%2944%3A8%26lt%3B1800%3A%3AAID-ART317%26gt%3B3.0.CO%3B2-1%2Ffull"},{"key":"ref16","unstructured":"Loeys, B. L., Chen, J., Neptune, E. R., Judge, D. P., Podowski, M., Holm, T., Meyers, J., Leitch, C. C., Katsanis, N., Sharifi, N., Xu, F. L., Myers, L. A., Spevak, P. J., Cameron, D. E., De Backer, J., Hellemans, J., Chen, Y., Davis, E. C., Webb, C. L., Kress, W., Coucke, P., Rifkin, D. B., De Paepe, A. M. and Dietz, H. C. (2005) A syndrome of altered cardiovascular, craniofacial, neurocognitive and skeletal development caused by mutations in TGFBR1 or TGFBR2. Nature genetics 37, 275-281. d093f82f87573d6553a03346be0c79dchttp%3A%2F%2Fheart.bmj.com%2Flookup%2Fexternal-ref%3Faccess_num%3D10.1038%2Fng1511%26amp%3Blink_type%3DDOI"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Mendioroz, M., Fernandez-Cadenas, I., Del Rio-Espinola, A., Rovira, A., Sole, E., Fernandez-Figueras, M. T., Garcia-Patos, V., Sastre-Garriga, J., Domingues-Montanari, S., Alvarez-Sabin, J. and Montaner, J. (2010) A missense HTRA1 mutation expands CARASIL syndrome to the Caucasian population. Neurology 75, 2033-2035. 78335879809396d6a212d9971c521590http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F21115960","DOI":"10.1212\/WNL.0b013e3181ff96ac"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Nishimoto, Y., Shibata, M., Nihonmatsu, M., Nozaki, H., Shiga, A., Shirata, A., Yamane, K., Kosakai, A., Takahashi, K., Nishizawa, M., Onodera, O. and Suzuki, N. (2011) A novel mutation in the HTRA1 gene causes CARASIL without alopecia. Neurology 76, 1353-1355. f17c542d6eb2a4da58c88cfd0d4386ebhttp%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F21482952","DOI":"10.1212\/WNL.0b013e318215281d"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Nozaki, H., Nishizawa, M. and Onodera, O. (2014) Features of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy. Stroke 45, 3447-3453. caacb836b7f25b58740acc1d166600a2http%3A%2F%2Feuropepmc.org%2Fabstract%2Fmed%2F25116877","DOI":"10.1161\/STROKEAHA.114.004236"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Nozaki, H., Sekine, Y., Fukutake, T., Nishimoto, Y., Shimoe, Y., Shirata, A., Yanagawa, S., Hirayama, M., Tamura, M., Nishizawa, M. and Onodera, O. (2015) Characteristic features and progression of abnormalities on MRI for CARASIL. Neurology 85, 459-463. 7ad38b8b6007ecdb828dca4eb828d202http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F26138950","DOI":"10.1212\/WNL.0000000000001803"},{"key":"ref21","unstructured":"Oka, C., Tsujimoto, R., Kajikawa, M., Koshiba-Takeuchi, K., Ina, J., Yano, M., Tsuchiya, A., Ueta, Y., Soma, A., Kanda, H., Matsumoto, M. and Kawaichi, M. (2004) HtrA1 serine protease inhibits signaling mediated by Tgfbeta family proteins. Development 131, 1041-1053. 8636e9c0b8eda94e4b91398c43c7f097http%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F14973287"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Ou, J., Pan, F., Geng, P., Wei, X., Xie, G., Deng, J., Pang, X. and Liang, H. (2012) Silencing fibronectin extra domain A enhances radiosensitivity in nasopharyngeal carcinomas involving an FAK\/Akt\/JNK pathway. International Journal of Radiation Oncology, Biology, Physics 82, e685-e691. 38535c62c14cacfae52193a978143c22http%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F22208970","DOI":"10.1016\/j.ijrobp.2011.09.040"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Pannu, H., Fadulu, V. T., Chang, J., Lafont, A., Hasham, S. N., Sparks, E., Giampietro, P. F., Zaleski, C., Estrera, A. L., Safi, H. J., Shete, S., Willing, M. C., Raman, C. S. and Milewicz, D. M. (2005) Mutations in transforming growth factor-beta receptor type II cause familial thoracic aortic aneurysms and dissections. Circulation 112, 513-520. de35265077aaea63a678636e58353367http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS1062145805010664","DOI":"10.1161\/CIRCULATIONAHA.105.537340"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Preethish-Kumar, V., Nozaki, H., Tiwari, S., Vengalil, S., Bhat, M., Prasad, C., Onodera, O., Uemura, M., Doniparthi, S., Saini, J., Nashi, S., Polavarapu, K. and Nalini, A. (2017) CARASIL families from India with 3 novel null mutations in the HTRA1 gene. Neurology 89, 2392-2394. 3c73cc18f4a36e5ebe544053dd3acf67http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F29101275","DOI":"10.1212\/WNL.0000000000004710"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Sattar, A., Rooney, P., Kumar, S., Pye, D., West, D. C., Scott, I. and Ledger, P. (1994) Application of angiogenic oligosaccharides of hyaluronan increases blood vessel numbers in rat skin. The Journal of Investigative Dermatology 103, 576-579. 73d6fd812e0b80aa54272d0f6f17f7fahttp%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F7523533","DOI":"10.1111\/1523-1747.ep12396880"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Shiga, A., Nozaki, H., Yokoseki, A., Nihonmatsu, M., Kawata, H., Kato, T., Koyama, A., Arima, K., Ikeda, M., Katada, S., Toyoshima, Y., Takahashi, H., Tanaka, A., Nakano, I., Ikeuchi, T., Nishizawa, M. and Onodera, O. (2011) Cerebral small-vessel disease protein HTRA1 controls the amount of TGF-beta1 via cleavage of proTGF-beta1. Human Molecular Genetics 20, 1800-1810. 34b92c501ce6169815615e05be3164cbhttp%3A%2F%2Fhmg.oxfordjournals.org%2Fcontent%2F20%2F9%2F1800.full","DOI":"10.1093\/hmg\/ddr063"},{"key":"ref27","unstructured":"ten Dijke, P. and Arthur, H., M. (2007) Extracellular control of TGFbeta signalling in vascular development and disease. Molecular Cell Biology 8, 857-869. d84f5ab2a942506f5ebf3f790837fdc3http%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F17895899"},{"key":"ref28","unstructured":"Wang, X. L., Li, C. F., Guo, H. W. and Cao, B. Z. (2012) A novel mutation in the HTRA1 gene identified in Chinese CARASIL pedigree. CNS Neuroscience & Therapeutics 18, 867-869. 97b6d87c7ec1872a1b6bc1fe24054ca0http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1111%2Fj.1755-5949.2012.00373.x%2Ffull"},{"key":"ref29","unstructured":"Wang, Z., Lee, S., Oliver, D., Yuan, S., Tang, C., Wang, Y., Zheng, H. and Yan, W. (2018) Prps1l1, a testis-specific gene, is dispensable for mouse spermatogenesis. Molecular Reproduction and Development 85, 802-804. 5b5fa34d3fa4c8d584f8a34cc762b208http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1002%2Fmrd.23053%2Fabstract"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Wassmer, S. C., de Souza, J. B., Frere, C., Candal, F. J., Juhan-Vague, I. and Grau, G. E. (2006) TGF-beta1 released from activated platelets can induce TNF-stimulated human brain endothelium apoptosis: a new mechanism for microvascular lesion during cerebral malaria. Journal of Immunology 176, 1180-1184. 9ff19baba27ceb812f44d5e5e885c6e9http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F16394007","DOI":"10.4049\/jimmunol.176.2.1180"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Wu, Y., Wu, J., Lee, D. Y., Yee, A., Cao, L., Zhang, Y., Kiani, C., Yang, B. B. (2005) Versican protects cells from oxidative stress-induced apoptosis. Journal of the International Society for Matrix Biology 24, 3-13. 19a7f7a26b3bde7b9a738abf4caf44d5http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS0945053X04001581","DOI":"10.1016\/j.matbio.2004.11.007"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Xie, F. and Zhang, L. S. (2018) A Chinese CARASIL Patient Caused by Novel Compound Heterozygous Mutations in HTRA1. National Stroke Association 27, 2840-2842.","DOI":"10.1016\/j.jstrokecerebrovasdis.2018.06.017"},{"key":"ref33","unstructured":"Yan, X., Wu, J., Jiang, Q., Cheng, H., Han, J. J. and Chen, Y. G. (2018) CXXC5 suppresses hepatocellular carcinoma by promoting TGF-beta-induced cell cycle arrest and apoptosis. Journal of Molecular Cell Biology 10, 48-59. efcd9b752bef12ccd6e53a6ddfd66270http%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F29036306"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"Yanagawa, S., Ito, N, Arima, K. and Ikeda, S. ( 2002) Cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy. Neurology 58, 817-820. c56644a5a5e0e1e53d75dcdccfa11882http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F11889251","DOI":"10.1212\/WNL.58.5.817"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"Zhang, L., Jia, R., Palange, N. J., Satheka, A. C., Togo, J., An, Y., Humphrey, M., Ban, L., Ji, Y., Jin, H., Feng, X. and Zheng, Y. (2015) Large genomic fragment deletions and insertions in mouse using CRISPR\/Cas9. PLoS One 10, e0120396. 394d5030015bde9a94fa9f12886fac97http%3A%2F%2Feuropepmc.org%2Farticles%2FPMC4372442","DOI":"10.1371\/journal.pone.0120396"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"Zhang, L., Schallert, T., Zhang, Z. G., Jiang, Q., Arniego, P., Li, Q., Lu, M. and Chopp, M. (2002) A test for detecting long-term sensorimotor dysfunction in the mouse after focal cerebral ischemia. Journal of Neuroscience Methods 117, 207-214. 15cab650e222374e78801c0f64e71b78http%3A%2F%2Feuropepmc.org%2Fabstract%2FMED%2F12100987","DOI":"10.1016\/S0165-0270(02)00114-0"}],"container-title":["Reviews in Cardiovascular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.imrpress.com\/journal\/RCM\/20\/3\/10.31083\/j.rcm.2019.03.31813","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T16:59:18Z","timestamp":1732726758000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.imrpress.com\/journal\/RCM\/20\/3\/10.31083\/j.rcm.2019.03.31813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,30]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019]]}},"alternative-id":["2153-8174-20-3-179"],"URL":"https:\/\/doi.org\/10.31083\/j.rcm.2019.03.31813","relation":{},"ISSN":["1530-6550","2153-8174"],"issn-type":[{"type":"print","value":"1530-6550"},{"type":"electronic","value":"2153-8174"}],"subject":[],"published":{"date-parts":[[2019,9,30]]},"article-number":"179"}}