{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:29:40Z","timestamp":1793316580203,"version":"4.5.0"},"reference-count":32,"publisher":"IMR Press","issue":"2","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Integr. Neurosci."],"abstract":"<jats:p>Background: Ischemic stroke is a prevalent global condition and its associated brain damage poses a significant threat to patient survival and outcomes. The underlying mechanisms of ischemic stroke-induced brain injury remain elusive, necessitating further investigation. Methods: Ischemic stroke models were established using middle cerebral artery occlusion (MCAO) in animals and oxygen-glucose deprivation and reperfusion (OGD-R) in cells. Phospholipase B domain-containing protein 1 (PLBD1) expression in these models was assessed via western blotting analysis, reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR), and cell immunofluorescence. A comprehensive evaluation, incorporating cellular lactate dehydrogenase (LDH) release assays, glycolysis metabolism kits, RT-qPCR, western blotting, triphenyl tetrazolium chloride (TTC) staining, neurological scoring, brain tissue water content measurement, and creatine kinase-MB (CK-MB) analysis, was conducted to determine the impact of PLBD1 on brain injury. Potential lactylation sites in PLBD1 were predicted using the DeepKla database, with western blotting and co-immunoprecipitation (Co-IP) confirming the lactylation site. Results: PLBD1 was significantly upregulated in the brain tissue of MCAO animal models and OGD-R-treated cells. PLBD1 knockdown markedly mitigated OGD-R-induced cellular injury, suppressed glycolysis in vitro, and reversed MCAO-induced brain damage in vivo. Furthermore, lactylation at the K155 site of PLBD1 enhanced its expression in response to elevated lactate levels following OGD-R treatment. These results indicated that the upregulation of PLBD1 via K155 site lactylation plays a pivotal role in exacerbating ischemic stroke-induced brain damage. Conclusion: Targeting the lactate\/PLBD1 axis presents a promising therapeutic strategy for ischemic stroke management.<\/jats:p>","DOI":"10.31083\/jin25949","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T09:52:29Z","timestamp":1740736349000},"source":"Crossref","is-referenced-by-count":17,"title":["Lactylation of PLBD1 Facilitates Brain Injury Induced by Ischemic Stroke"],"prefix":"10.31083","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3825-7293","authenticated-orcid":true,"given":"Faming","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Neurology, Renmin Hospital, Hubei University of Medicine, 442000 Shiyan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2692-9308","authenticated-orcid":true,"given":"Guanghui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology, Renmin Hospital, Hubei University of Medicine, 442000 Shiyan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1804-5737","authenticated-orcid":true,"given":"Xiaoli","family":"Li","sequence":"first","affiliation":[{"name":"Department of Neurology, Renmin Hospital, Hubei University of Medicine, 442000 Shiyan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7035-6162","authenticated-orcid":true,"given":"Xiaodong","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Neurology, Renmin Hospital, Hubei University of Medicine, 442000 Shiyan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3026-6293","authenticated-orcid":true,"given":"Yinyin","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Neurology, Renmin Hospital, Hubei University of Medicine, 442000 Shiyan, Hubei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Shademan B, Avci CB, Karamad V, Soureh GJ, Olia JBH, Esmaily F, et al. 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