{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:13:47Z","timestamp":1788444827240,"version":"build-2803163510"},"reference-count":36,"publisher":"IMR Press","issue":"8","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Xin'an Medicine and Modernization of Traditional Chinese Medicine Research Institute","award":["2023CXMMTCM007"],"award-info":[{"award-number":["2023CXMMTCM007"]}]},{"name":"Key Projects of Scientific Research in Higher Education Institutions of Anhui Province","award":["KJ2020A0608"],"award-info":[{"award-number":["KJ2020A0608"]}]},{"name":"Anhui Provincial University STEM Faculty Enterprise Secondment and Practical Training Program Project","award":["2025jsqygz39"],"award-info":[{"award-number":["2025jsqygz39"]}]},{"name":"Anhui Provincial Key Scientific Research Project Foundation","award":["2025AHGXZK30634"],"award-info":[{"award-number":["2025AHGXZK30634"]}]},{"name":"Anhui Provincial Cultivation Project for Outstanding Young Teachers","award":["YQYB2024046"],"award-info":[{"award-number":["YQYB2024046"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmazie"],"abstract":"<jats:p>Objective:This study aimed to identify the active fraction of Polyporus umbellatus extract (PUE) for the treatment of hyperuricemia (HUA) and elucidate the associated mechanism of action.Methods:HUA was induced in mice using hypoxanthine and potassium oxonate. Serum uric acid (UA), urinary UA, creatinine (CRE), blood urea nitrogen (BUN), xanthine oxidase (XOD) activity, inflammatory cytokines (Interleukin-6 (IL-6), Tumor necrosis factor-\u03b1 (TNF-\u03b1)), and renal urate transporters (Uric acid transporter 1 (URAT1), Organic anion transporter 1 (OAT1)) were subsequently measured. The active fraction was identified by bioactivity-guided fractionation using D101 macroporous resin chromatography. Two major compounds were isolated and evaluated for anti-inflammatory (nitric oxide (NO) production, cytokine mRNA expression) and antioxidant (Malondialdehyde (MDA), Superoxide dismutase (SOD)) activities in monosodium urate (MSU)-induced RAW264.7 macrophages.Results:PUE significantly ameliorated hyperuricemia in HUA mice, reducing serum UA, CRE, BUN, and renal URAT1 expression; increasing urinary UA excretion and OAT1 expression; inhibiting XOD activity; attenuating renal inflammation and histopathological damage. The 95% ethanol eluate was identified as the most active fraction and yielded ergosterol (A) and 5\u03b1,8\u03b1-epidioxy-(22E,24R)-ergosta-6,22-dien-3\u03b2-ol (B). Both compounds suppressed MSU-induced NO release, proinflammatory cytokine expression, and oxidative stress.Conclusion:The 95% ethanol eluate of P. umbellatus exerts anti-hyperuricemic effects through multiple mechanisms, possibly related to XOD inhibition, anti-inflammatory activity, antioxidant protection, and regulation of renal uric acid transport proteins. Ergosterol derivatives are proposed as the potential active components in this extract.<\/jats:p>","DOI":"10.31083\/pharmazie54200","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T13:26:12Z","timestamp":1788441972000},"source":"Crossref","is-referenced-by-count":0,"title":["The Active Fraction From Polyporus umbellatus Against Hyperuricemia"],"prefix":"10.31083","volume":"81","author":[{"given":"Jian","family":"Luo","sequence":"first","affiliation":[{"name":"Market Regulation Bureau in Lujiang County, 231500 Lujiang, Anhui, China"},{"name":"Provincial Engineering Laboratory for Screening and Reevaluation of Active Compounds of Herbal Medicines in Southern Anhui, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Provincial Engineering Laboratory for Screening and Reevaluation of Active Compounds of Herbal Medicines in Southern Anhui, 241002 Wuhu, Anhui, China"},{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Run-ze","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-yao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-qi","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-yan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dao-yin","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-wen","family":"Gong","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-hao","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7330-5855","authenticated-orcid":true,"given":"Si-hui","family":"Nian","sequence":"additional","affiliation":[{"name":"Provincial Engineering Laboratory for Screening and Reevaluation of Active Compounds of Herbal Medicines in Southern Anhui, 241002 Wuhu, Anhui, China"},{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3074-7255","authenticated-orcid":true,"given":"Ling-yun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Provincial Engineering Laboratory for Screening and Reevaluation of Active Compounds of Herbal Medicines in Southern Anhui, 241002 Wuhu, Anhui, China"},{"name":"College of Pharmacy, Wannan Medical University, 241002 Wuhu, Anhui, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"16242","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","unstructured":"Li X, Chen Z, Zhang Y, Fan C, Chen L, Xu X, et al. 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