{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T07:15:03Z","timestamp":1697872503186},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,1,11]],"date-time":"2011-01-11T00:00:00Z","timestamp":1294704000000},"content-version":"vor","delay-in-days":9506,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[1985,1]]},"abstract":"<jats:p><jats:bold>Abstract. <\/jats:bold> A sensitive catalytic assay for placental alkaline phosphatase (PLAP) was used to quantify enzyme levels in sera from women with malignant gynecological tumors, i.e. cervical carcinoma, ovarian carcinoma and carcinoma of the breast. Values were compared with those of a reference group of 155 healthy individuals. In this group, basic mean levels of the enzyme amounted to 0.06\u00b10.08\u03bcmoles \u00d7 min<jats:sup>\u22121<\/jats:sup> \u00d7 1<jats:sup>\u22121<\/jats:sup> (mean\u00b1SD), which corresponds to 0.3\u00b10.4 ng\/ml (mean\u00b1SD) purified placental alkaline phosphatase. Enzyme levels were not significantly affected by age, whereas sex gave slightly higher values for women in all age groups. This difference was statistically significant (p&lt; 0.001). The discriminating capacity of PLAP as a tumor marker was analysed with this assay and compared in sensitivity with a radio\u2010immunoassay. A cut\u2010off level of 0.2 \u03bcmoles \u00d7 min<jats:sup>\u22121<\/jats:sup> \u00d7 1<jats:sup>\u22121<\/jats:sup>, corresponding to 1 ng\/ml enzyme protein as measured by the radio\u2010immunoassay, detected 23\u201068% of the tumor patients, giving false\u2010positive results from the control group in the order of 5%. Corresponding values at a cut\u2010off level of 0.3 \u03bcmolesxmin<jats:sup>\u22121<\/jats:sup> \u00d7 1<jats:sup>\u22121<\/jats:sup> (1.5 ng\/ml) gave values 20\u201044% and 2%, respectively. The present investigation emphasizes that sensitivity in assays of placental alkaline phosphatase, whether catalytic or immu\u2010nological, must not exceed ng\u2010level.<\/jats:p>","DOI":"10.3109\/00016348509154699","type":"journal-article","created":{"date-parts":[[2009,12,8]],"date-time":"2009-12-08T17:57:36Z","timestamp":1260295056000},"page":"99-103","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["ON THE VALUE OF PLACENTAL ALKALINE PHOSPHATASE AS A MARKER FOR GYNECOLOGICAL MALIGNANCY"],"prefix":"10.1111","volume":"64","author":[{"given":"T.","family":"Stigbrand","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. \u00c5.","family":"Holmgren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Jeppsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. G.","family":"Damber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"von Schoultz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,11]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"150","article-title":"Serum alkaline phosphatase isoenzyme of human neo\u2010plastic cell origin","volume":"28","author":"Fishman W H.","year":"1968","journal-title":"Cancer Res"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1978.tb03970.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910240304"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910230608"},{"key":"e_1_2_1_6_1","first-page":"269","article-title":"Placenta\u2010like alkaline phosphatase in gynecological cancers","volume":"36","author":"Kellen J A.","year":"1976","journal-title":"Cancer Res"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(75)90252-1"},{"key":"e_1_2_1_8_1","first-page":"2413","article-title":"A radioimmunoassay for pla\u2010cental\u2010type alkaline phosphatase","volume":"32","author":"Jacoby B.","year":"1972","journal-title":"Cancer Res"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196910022811403"},{"key":"e_1_2_1_10_1","first-page":"1574","article-title":"A sensitive immunochemical method for the determination of the R?gan isoenzyme in serum","volume":"33","author":"Usategui\u2010Gomez M.","year":"1973","journal-title":"Cancer Res"},{"key":"e_1_2_1_11_1","first-page":"1706","article-title":"A simple radioimmunoassay of human placental alkaline phosphatase (Regan isoenzyme) using specific antibody polymers","volume":"35","author":"Chang C\u2010H","year":"1975","journal-title":"Cancer Res"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(77)90113-9"},{"key":"e_1_2_1_13_1","first-page":"1657","article-title":"Phenotypes of the Regan isoenzyme and identity between the placental D\u2010variant and the Nagao isoenzyme","volume":"33","author":"Inglis N R.","year":"1973","journal-title":"Cancer Res"},{"key":"e_1_2_1_14_1","first-page":"729","article-title":"Increased incidence of placental\u2010like alkaline phosphatase activity in breast and genitourinary cancer","volume":"34","author":"Cadeun B J.","year":"1974","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_1","first-page":"211","article-title":"Markers for ovarian cancer: Regan isoenzyme and other glycopro\u2010tein","volume":"2","author":"Fishman W H.","year":"1975","journal-title":"Seminars in Oncology"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910240608"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(78)90108-0"},{"key":"e_1_2_1_18_1","first-page":"177","article-title":"Alkaline phosphatase activity in human thoracic duct lymph","volume":"129","author":"Blomstrand R.","year":"1965","journal-title":"Acta Chir Scand"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(70)90296-5"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/jnci\/58.3.489","article-title":"Multiple antigens as marker substances in germinal tumors of the testis","volume":"58","author":"Wahren B.","year":"1977","journal-title":"J Nat Cancer Inst"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-78-1-39"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197611252952207"},{"key":"e_1_2_1_23_1","first-page":"2014","volume":"27","author":"Millan J L.","year":"1981","journal-title":"\u201cSandwich\u201d enzyme immuno-assay for placental alkaline phosphatase. Clin Chem"}],"container-title":["Acta Obstetricia et Gynecologica Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.3109%2F00016348509154699","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.3109\/00016348509154699","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T06:53:20Z","timestamp":1697784800000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.3109\/00016348509154699"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,1]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,1]]}},"alternative-id":["10.3109\/00016348509154699"],"URL":"https:\/\/doi.org\/10.3109\/00016348509154699","archive":["Portico"],"relation":{},"ISSN":["0001-6349","1600-0412"],"issn-type":[{"value":"0001-6349","type":"print"},{"value":"1600-0412","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,1]]},"assertion":[{"value":"2011-01-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}