{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:38:38Z","timestamp":1760204318766},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,1,26]],"date-time":"2010-01-26T00:00:00Z","timestamp":1264464000000},"content-version":"vor","delay-in-days":5779,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[1994,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p> <jats:bold>Objective<\/jats:bold>: The objective is to determine the contributions of electrostatic charge, endothelial cell adhesion glycoproteins (P\u2010 and E\u2010selectins), and histamine to lactoferrin\u2010induced leukocyte adhesion in postcapillary venules.<\/jats:p><jats:p> <jats:bold>Methods<\/jats:bold>: Rat mesentery was prepared for intravital microscopic observation. Erythrocyte velocity, vessel diameter, leukocyte rolling velocity, number of adherent and emigrated leukocytes, and velocity, flux, and number of rolling leukocytes were monitored in mesenteric venules (25\u201335 \u03bcm initial diameter). After control measurements were obtained for all parameters, lactoferrin, other cationic proteins (histone or \u03b1\u2010chymotrypsinogen A), or transferrin (an anionic iron\u2010binding protein) were infused into the superior mesenteric artery, with repeat measurements taken 20 min into the infusion period. In other lactoferrin experiments, animals were treated with either a monoclonal antibody (MAb) directed against P\u2010 or E\u2010selectin, an H<jats:sub>1<\/jats:sub>\u2010 or H<jats:sub>2<\/jats:sub>\u2010histamine receptor antagonist, or diamine oxidase (histaminase).<\/jats:p><jats:p> <jats:bold>Results<\/jats:bold>: Increased numbers of rolling and adherent leukocytes were observed during infusion of lactoferrin, histone, or \u03b1\u2010chymotrypsinogen A but not with transferrin. The leukocyte\u2010endothelial cell adhesion (LECA) elicited by lactoferrin was substantially greater than that induced by histone and \u03b1\u2010chymotrypsinogen A. The P\u2010selectin MAb completely prevented lactoferrin\u2010induced LECA, whereas the E\u2010selectin MAb had no effect. Diamine oxidase and the H<jats:sub>1\u2212<\/jats:sub> (but not the H<jats:sub>2\u2212<\/jats:sub>) receptor antagonist were also effective in attenuating lactoferrin\u2010induced LECA.<\/jats:p><jats:p> <jats:bold>Conclusions<\/jats:bold>: These results indicate that lactoferrin\u2010induced LECA results from histamine\u2010mediated expression of P\u2010selectin on venular endothelial cells. The cationic nature of lactoferrin accounts for only a small part of its proadhesive actions.<\/jats:p>","DOI":"10.3109\/10739689409148259","type":"journal-article","created":{"date-parts":[[2008,4,24]],"date-time":"2008-04-24T16:40:28Z","timestamp":1209055228000},"page":"27-34","source":"Crossref","is-referenced-by-count":8,"title":["Mechanisms of Lactoferrin\u2010induced Leukocyte\u2010Endothelial Cell Adhesion in Postcapillary Venules"],"prefix":"10.1111","volume":"1","author":[{"given":"Hiroshi","family":"Asako","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iwao","family":"Kurose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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