{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:26:59Z","timestamp":1762100819815},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,1,26]],"date-time":"2010-01-26T00:00:00Z","timestamp":1264464000000},"content-version":"vor","delay-in-days":4530,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microcirculation"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:bold>Objective<\/jats:bold>: Neutrophilic leukocyte rolling on postcapillary endothelium requires membrane interaction between SLe<jats:sup>x<\/jats:sup>\u2010containing ligands on neutrophils and P\u2010selectin on endothelial cells. The current sequence of studies was performed to explore the hypothesis that the leukocyte\u2010endothelial rolling interaction not only precedes leukocyte migration but also is accompanied by oxygen free radical production and interstitial cell death in the rat mesentery.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold>: The ratio of leukocyte rolling velocity on the endothelium of postcapillary venules and centerline red cell velocity was determined after topical application of platelet activating factor (PAF; 10<jats:sup>\u22128<\/jats:sup> mol\/L). Superoxide formation was determined by an <jats:italic>in situ<\/jats:italic> nitroblue tetrazolium reduction to dark blue formazan crystals in the <jats:italic>in situ<\/jats:italic> mesentery preparation, and cell death was detected by nuclear staining with propidium iodide.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold>: Leukocyte rolling and subsequent adhesion was inhibited with a monoclonal antibody against P\u2010selectin, PB1.3. Superoxide formation, as well as parenchymal cell death, was significantly enhanced in the mesentery after stimulation with platelet activating factor, and both could be significantly attenuated by reduction of the rolling leukocyte\u2010endothelial interaction with PB1.3.<\/jats:p><jats:p><jats:bold>Conclusions<\/jats:bold>: These results provide direct evidence that the interaction of leukocytes and endothelium followed by migration of leukocytes into the interstitium is accompanied by enhanced oxidative stress and parenchymal cell death. Early interruption of the interaction provides significant protection even in the presence of a proinflammatory stimulus.<\/jats:p>","DOI":"10.3109\/10739689709146799","type":"journal-article","created":{"date-parts":[[2008,4,24]],"date-time":"2008-04-24T22:34:46Z","timestamp":1209076486000},"page":"349-357","source":"Crossref","is-referenced-by-count":27,"title":["Attenuation of Oxygen Free Radical Formation and Tissue Injury During Experimental Inflammation by P\u2010selectin Blockade"],"prefix":"10.1111","volume":"4","author":[{"given":"Frank A.","family":"Delano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Forrest","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert W.","family":"Schmid\u2010Sch\u00f6nbein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,26]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"H415","article-title":"Circulating neutrophil kinetics during tolerance in hemorrhagic shock using bacterial lipopolysaccaride","volume":"266","author":"Barroso\u2010Aranda J.","year":"1994","journal-title":"Am J Physiol"},{"key":"e_1_2_1_3_1","first-page":"H846","article-title":"Transformation of neutrophils as indicator of irreversibility in hemorrhagic shock","volume":"257","author":"Barroso\u2010Aranda J.","year":"1989","journal-title":"Am J Physiol"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199500252-00006"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1983.tb07343.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.66.5.1436"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1152\/jappl.1993.75.6.2529","article-title":"Pulmonary microvascular injury after intestinal ischemia\u2010reperfusion: role of P\u2010selectin","volume":"75","author":"Carden DL","year":"1993","journal-title":"J Appl Physiol"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1987.tb11336.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1182\/blood.V82.4.1308.1308","article-title":"P\u2010selectin mediates spontaneous leukocyte rolling","volume":"82","author":"Dor\u00e9 M.","year":"1993","journal-title":"in vivo. 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