{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T06:03:33Z","timestamp":1671689013994},"reference-count":21,"publisher":"Czech Society of Ophthalmology of the CzMA","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CSO"],"published-print":{"date-parts":[[2022,12,5]]},"abstract":"<jats:p>Aims: The main aim of our study was to demonstrate the difference in endothelial cell density between a\u00a0group of keratoconus contact lens users and non-contact lens users (without keratoconus).\nMaterial and methods: In our study we had data from 96 subjects with an average age of 40.5 \u00b114.05 years. For the purposes of our study, we worked with each eye separately for each subject (n\u00a0=\u00a0192). Keratoconus (research group) was diagnosed in 97 eyes. The mean age of the patients in the research group was 41.9 \u00b110.6 years. Keratoconus was not diagnosed in the remaining 95 eyes (control group). The mean age of the patients in the control group was 39.5 \u00b116.6 years. In the keratoconus group, the patients wore hard contact lenses (HCL) in a\u00a0total of 43 eyes, hybrid contact lenses (HbCL) in 48 eyes and soft contact lenses (SCL) in 6 eyes. The average total period of contact lens use in the research group was 10.6 \u00b12.36 years.\nResults: The average number of endothelial cells in the research group was 2607.11 \u00b1298.45 cells per mm2. The average number of endothelial cells in the control group was 2831.94 \u00b1523.51 cells per mm2. We tested these two variables using a\u00a0T-test, which showed a\u00a0statistically significant difference (p\u00a0&lt;\u00a00.001). A\u00a0statistically significant difference in the mean endothelial cell count related to patient age and use \/ non-use of contact lenses of two types was demonstrated only in the under-40 group in a\u00a0comparison of the non-contact and keratoconus group with the keratoconus group using HCL \n(p\u00a0=\u00a00.02). A\u00a0statistically significant difference in the length of contact lens wear was demonstrated between the keratoconus-free group with or without SCL and the keratoconus group when wearing HCL or HbCL for more than 20 years (p\u00a0=\u00a00.01 and p\u00a0=\u00a00.02). For HbCL users, this difference was demonstrated also after 15 years of wearing (p\u00a0=\u00a00.001).\nConclusion: From our results we can conclude that there is a\u00a0difference in endothelial cell density between patients with and without keratoconus.<\/jats:p>","DOI":"10.31348\/2022\/30","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T18:07:09Z","timestamp":1671646029000},"page":"306-311","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of Corneal Endothelial Layer in Contact Lens Wearers with the Aid of an Endothelial Microscope"],"prefix":"10.31348","volume":"78","author":[{"given":"Petr","family":"Vesel\u00fd","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel","family":"Bene\u0161","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Sokolov\u00e1 \u0160idlov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Z\u00e1d\u011brov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Do\u0161kov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"16213","published-online":{"date-parts":[[2022,12,30]]},"reference":[{"key":"ref0","unstructured":"Sayegh RR, Benetz BA, Lass JH. Specular Microscopy. In: MANNIS MJ, Holland EJ. Cornea. 4th edition. N.Y.: Elsevier, 2017, pp. 160-179. ISBN 978-0-3233-5757-9."},{"key":"ref1","unstructured":"Vlkov\u00e1 E, Hlinomazov\u00e1 Z. Rizikov\u00e1 keratoplastika. Brno: Masarykova univerzita, 1999. 76 s. ISBN 80-210-2108-X."},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"Galgauskas S, Norvydait\u0117 D, Krasauskait\u0117 D, Stech S, A\u0161oklis RS. Age-related changes in corneal thickness and endothelial characteristics. Clin Interv Aging. 2013;8:1445-1450.","DOI":"10.2147\/cia.s51693"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"Ko MK, Park WK, Lee JH, Chi JG. A histomorphometric study of corneal endothelial cells in normal human fetuses. Exp Eye Res. 2001;72(4):403-409.","DOI":"10.1006\/exer.2000.0964"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"Ayala G, Diaz ME, Martinez-Costa L. Granulometric moments and corneal endothelium status. 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