{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T16:07:53Z","timestamp":1789142873740,"version":"build-2803163510"},"reference-count":26,"publisher":"University of Toronto Press Inc. (UTPress)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Invest Med"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Lung cancer is the malignancy with the highest mortality rate globally. This study aimed to investigate the expression of carbonic anhydrase IX (\n                      <jats:italic toggle=\"yes\">CA9<\/jats:italic>\n                      ) and\n                      <jats:italic toggle=\"yes\">SEC61G<\/jats:italic>\n                      in non-small cell lung cancer (NSCLC) tissues and adjacent lung tissues and to analyze the relationships of these genes with clinicopathological features to identify potential biomarkers and therapeutic targets.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Bioinformatic analysis was performed using the TIMER, TCGA, and GTEx databases to evaluate the differential expression of\n                      <jats:italic toggle=\"yes\">CA9<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">SEC61G<\/jats:italic>\n                      in NSCLC. The associations of these genes with tumour stage were further analyzed via GEPIA. For experimental validation, immunohistochemistry was conducted on 50 NSCLC tissue samples and 37 adjacent tissue samples collected from our institution.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The expression levels of both\n                      <jats:italic toggle=\"yes\">CA9<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">SEC61G<\/jats:italic>\n                      were significantly higher in NSCLC tissues than in adjacent tissues. The expression of these genes was positively correlated with advanced T stage, lymph node metastasis, distant metastasis, and tumour stage. A significant positive correlation was also observed between\n                      <jats:italic toggle=\"yes\">CA9<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">SEC61G<\/jats:italic>\n                      expression in NSCLC tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">CA9<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">SEC61G<\/jats:italic>\n                      are upregulated in NSCLC, and their expression correlates with aggressive clinicopathological features, suggesting their involvement in tumour progression. The coexpression of these genes suggests a potential synergistic effect in NSCLC, adding to the existing evidence of their utility as significant indicators for predicting disease stage and prognosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3138\/cim-2026-0038","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:08:52Z","timestamp":1787854132000},"page":"17-26","source":"Crossref","is-referenced-by-count":0,"title":["Expression and clinical significance of\n                    <i>CA9<\/i>\n                    and\n                    <i>SEC61G<\/i>\n                    in non\u2013small cell lung cancer"],"prefix":"10.3138","volume":"49","author":[{"given":"Yu","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Bazhong Central Hospital, Bazhong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dehe","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Bazhong Central Hospital, Bazhong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Bazhong Central Hospital, Bazhong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Bazhong Central Hospital, Bazhong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoxiong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Bazhong Central Hospital, Bazhong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinghai","family":"He","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Bazhong Central Hospital, Bazhong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Bazhong Central Hospital, Bazhong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3526-8851","authenticated-orcid":true,"given":"Li","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1696","reference":[{"issue":"3","key":"e_1_3_9_2_2","first-page":"209","article-title":"Global Cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung H","year":"2021","unstructured":"Sung H, Ferlay J, Siegel RL, . 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