{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T05:16:36Z","timestamp":1783142196639,"version":"3.54.6"},"reference-count":35,"publisher":"The Society for Free Radical Research Japan","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Clin. Biochem. Nutr."],"published-print":{"date-parts":[[2026,7,1]]},"DOI":"10.3164\/jcbn.25-263","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T22:09:49Z","timestamp":1778278189000},"page":"104-110","source":"Crossref","is-referenced-by-count":0,"title":["Anti-aging effects of glycyrrhizin via maintaining Klotho levels"],"prefix":"10.3164","volume":"79","author":[{"given":"Shiho","family":"Ohnishi","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichi","family":"Hiramoto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuji","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Matsusaka R&amp;D Center, Cokey Co., Ltd."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shosuke","family":"Kawanishi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1739","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1 Cohn RL, Gasek NS, Kuchel GA, Xu M. The heterogeneity of cellular senescence: insights at the single-cell level. Trends Cell Biol 2023; 33: 9\u201317.","DOI":"10.1016\/j.tcb.2022.04.011"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2 Yamauchi S, Takahashi A. Cellular senescence: mechanisms and relevance to cancer and aging. J Biochem 2025; 177: 163\u2013169.","DOI":"10.1093\/jb\/mvae079"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3 Oikawa S, Kawanishi S. Site-specific DNA damage at GGG sequence by oxidative stress may accelerate telomere shortening. FEBS Lett 1999; 453: 365\u2013368.","DOI":"10.1016\/S0014-5793(99)00748-6"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4 Kawanishi S, Oikawa S. Mechanism of telomere shortening by oxidative stress. Ann N Y Acad Sci 2004; 1019: 278\u2013284.","DOI":"10.1196\/annals.1297.047"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5 Zhong S, Zhou Q, Yang J, et al. Relationship between the cGAS-STING and NF-\u03baB pathways-role in neurotoxicity. Biomed Pharmacother 2024; 175: 116698.","DOI":"10.1016\/j.biopha.2024.116698"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6 Xie X, Lian S, Yang W, et al. Natural products for the treatment of age-related macular degeneration: new insights focusing on mitochondrial quality control and cGAS\/STING pathway. J Pharm Anal 2025; 15: 101145.","DOI":"10.1016\/j.jpha.2024.101145"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7 Hao X, Wang C, Zhang R. Chromatin basis of the senescence-associated secretory phenotype. Trends Cell Biol 2022; 32: 513\u2013526.","DOI":"10.1016\/j.tcb.2021.12.003"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8 Lopes-Paciencia S, Saint-Germain E, Rowell MC, Ruiz AF, Kalegari P, Ferbeyre G. The senescence-associated secretory phenotype and its regulation. Cytokine 2019; 117: 15\u201322.","DOI":"10.1016\/j.cyto.2019.01.013"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9 Shin JW, Jang DH, Kim SY, et al. Propagation of senescent phenotypes by extracellular HMGB1 is dependent on its redox state. Metabolism 2025; 168: 156259.","DOI":"10.1016\/j.metabol.2025.156259"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10 Chen J, Li H, Huang Y, Tang Q. The role of high mobility group proteins in cellular senescence mechanisms. Front Aging 2024; 5: 1486281.","DOI":"10.3389\/fragi.2024.1486281"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11 Prud&apos;homme GJ, Kurt M, Wang Q. Pathobiology of the Klotho antiaging protein and therapeutic considerations. Front Aging 2022; 3: 931331.","DOI":"10.3389\/fragi.2022.931331"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12 Veronesi F, Borsari V, Cherubini A, Fini M. Association of Klotho with physical performance and frailty in middle-aged and older adults: a systematic review. Exp Gerontol 2021; 154: 111518.","DOI":"10.1016\/j.exger.2021.111518"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13 Buchanan S, Combet E, Stenvinkel P, Shiels PG. Klotho, aging, and the failing kidney. Front Endocrinol (Lausanne) 2020; 11: 560.","DOI":"10.3389\/fendo.2020.00560"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14 Wang G, Hiramoto K, Ma N, et al. Glycyrrhizin attenuates carcinogenesis by inhibiting the inflammatory response in a murine model of colorectal cancer. Int J Mol Sci 2021; 22: 2609.","DOI":"10.3390\/ijms22052609"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15 Fisher RP, Matheny L, Ankeny S, Qin L, Coleman LG Jr, Vetreno RP. Adolescent binge alcohol exposure accelerates Alzheimer\u2019s disease-associated basal forebrain neuropathology through proinflammatory HMGB1 signaling. Front Aging Neurosci 2025; 17: 1531628.","DOI":"10.3389\/fnagi.2025.1531628"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16 Liu S, Hu R, Du J, Li Y, Li X. Glycyrrhizin ameliorates vascular endothelial cell senescence by inhibiting HMGB1 in HFD\/STZ-induced diabetic rats and human umbilical vein endothelial cells. Eur J Pharmacol 2022; 931: 175196.","DOI":"10.1016\/j.ejphar.2022.175196"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17 Wang ZH, Hsieh CH, Liu WH, Yin MC. Glycyrrhizic acid attenuated glycative stress in kidney of diabetic mice through enhancing glyoxalase pathway. Mol Nutr Food Res 2014; 58: 1426\u20131435.","DOI":"10.1002\/mnfr.201300910"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18 Horigome H, Hirano T, Oka K. Therapeutic effect of glycyrrhetinic acid in MRL lpr\/lpr mice: implications of alteration of corticosteroid metabolism. Life Sci 2001; 69: 2429\u20132438.","DOI":"10.1016\/S0024-3205(01)01317-0"},{"key":"19","unstructured":"19 Pan YL. The effects of glycyrrhizin on acute pancreatitis in mice. Eur Rev Med Pharmacol Sci 2014; 18: 3943\u20133947."},{"key":"20","doi-asserted-by":"crossref","unstructured":"20 Hou S, Zhang T, Li Y, Guo F, Jin X. Glycyrrhizic acid prevents diabetic nephropathy by activating AMPK\/SIRT1\/PGC-1\u03b1 signaling in db\/db mice. J Diabetes Res 2017; 2017: 2865912.","DOI":"10.1155\/2017\/2865912"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21 Rubinek T, Modan-Moses D. Klotho and the growth hormone\/insulin-like growth factor 1 axis: novel insights into complex interactions. Vitam Horm 2016; 101: 85\u2013118.","DOI":"10.1016\/bs.vh.2016.02.009"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22 Song C, Hu Z, Xu D, et al. STING signaling in inflammaging: a new target against musculoskeletal diseases. Front Immunol 2023; 14: 1227364.","DOI":"10.3389\/fimmu.2023.1227364"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23 Schmitz CRR, Maurmann RM, Guma FTCR, Bauer ME, Barb\u00e9-Tuana FM. cGAS-STING pathway as a potential trigger of immunosenescence and inflammaging. Front Immunol 2023; 14: 1132653.","DOI":"10.3389\/fimmu.2023.1132653"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24 Yamamoto T, Isaka Y. Pathological mechanisms of kidney disease in ageing. Nat Rev Nephrol 2024; 20: 603\u2013615.","DOI":"10.1038\/s41581-024-00868-4"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25 Moreno JA, Izquierdo MC, Sanchez-Ni\u00f1o MD, et al. The inflammatory cytokines TWEAK and TNF\u03b1 reduce renal klotho expression through NF\u03baB. J Am Soc Nephrol 2011; 22: 1315\u20131325.","DOI":"10.1681\/ASN.2010101073"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26 Eu CH, Lim WY, Ton SH, bin Abdul Kadir K. Glycyrrhizic acid improved lipoprotein lipase expression, insulin sensitivity, serum lipid and lipid deposition in high-fat diet-induced obese rats. Lipids Health Dis 2010; 9: 81.","DOI":"10.1186\/1476-511X-9-81"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27 Ariadel-Cobo DG, Est\u00e9banez B, Gonz\u00e1lez-Arn\u00e1iz E, et al. Influence of Klotho protein levels in obesity and sarcopenia: a systematic review. Int J Mol Sci 2025; 26: 1915.","DOI":"10.3390\/ijms26051915"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28 Buend\u00eda P, Ram\u00edrez R, Aljama P, Carracedo J. Klotho prevents translocation of NF\u03baB. Vitam Horm 2016; 101: 119\u2013150.","DOI":"10.1016\/bs.vh.2016.02.005"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29 Yoke Yin C, So Ha T, Abdul Kadir K. Effects of glycyrrhizic acid on peroxisome proliferator-activated receptor gamma (PPAR\u03b3), lipoprotein lipase (LPL), serum lipid and HOMA-IR in rats. PPAR Res 2010; 2010: 530265.","DOI":"10.1155\/2010\/530265"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30 Zhang R, Zheng F. PPAR-\u03b3 and aging: one link through klotho? Kidney Int 2008; 74: 702\u2013704.","DOI":"10.1038\/ki.2008.382"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31 Korbecki J, Bobi\u0144ski R, Dutka M. Self-regulation of the inflammatory response by peroxisome proliferator-activated receptors. Inflamm Res 2019; 68: 443\u2013458.","DOI":"10.1007\/s00011-019-01231-1"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32 Salminen A, Kaarniranta K. AMP-activated protein kinase (AMPK) controls the aging process via an integrated signaling network. Ageing Res Rev 2012; 11: 230\u2013241.","DOI":"10.1016\/j.arr.2011.12.005"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33 Zhou L, Yi Y, Lin B, Qiu Z, Wang C, Li Y. Glycyrrhizic acid mitigates hepatocyte steatosis and inflammation through ACE2 stabilization via dual modulation of AMPK activation and MDM2 inhibition. Eur J Pharmacol 2025; 1002: 177817.","DOI":"10.1016\/j.ejphar.2025.177817"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34 Corrales P, Vidal-Puig A, Medina-G\u00f3mez G. PPARs and metabolic disorders associated with challenged adipose tissue plasticity. Int J Mol Sci 2018; 19: 2124.","DOI":"10.3390\/ijms19072124"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35 Vogt J, Wolf L, Hoelzle LE, Feger M, F\u00f6ller M. AMP-dependent kinase stimulates the expression of \u03b1Klotho. FEBS Open Bio 2024; 14: 1691\u20131700.","DOI":"10.1002\/2211-5463.13872"}],"container-title":["Journal of Clinical Biochemistry and Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jcbn\/79\/1\/79_25-263\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T04:33:17Z","timestamp":1783139597000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jcbn\/79\/1\/79_25-263\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.3164\/jcbn.25-263","relation":{},"ISSN":["0912-0009","1880-5086"],"issn-type":[{"value":"0912-0009","type":"print"},{"value":"1880-5086","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,1]]},"article-number":"25-263"}}