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Pathogenesis of diet-induced atopic dermatitis in hairless mice. Yakugaku Zasshi 2017; 137: 49\u201354. (in Japanese)","DOI":"10.1248\/yakushi.16-00200"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47 Shi X, Shang F, Zhang Y, Wang R, Jia Y, Li K. Persimmon oligomeric proanthocyanidins alleviate ultraviolet B-induced skin damage by regulating oxidative stress and inflammatory responses. Free Radic Res 2020; 54: 765\u2013776.","DOI":"10.1080\/10715762.2020.1843651"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48 Wu Q, Feng Y, Ouyang Y, et al. Inhibition of advanced glycation endproducts formation by lotus seedpod oligomeric procyanidins through RAGE-MAPK signaling and NF-\u03baB activation in high-AGEs-diet mice. Food Chem Toxicol 2021; 156: 112481.","DOI":"10.1016\/j.fct.2021.112481"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49 Dong L, Lee H, Liu Z, Woo ER, Lee DS. 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