{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:49:07Z","timestamp":1792118947706,"version":"4.4.0"},"reference-count":47,"publisher":"American Dairy Science Association","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T00:00:00Z","timestamp":1547424000000},"content-version":"vor","delay-in-days":2935,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Science"],"published-print":{"date-parts":[[2011,1]]},"DOI":"10.3168\/jds.2010-3668","type":"journal-article","created":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T09:59:01Z","timestamp":1293011941000},"page":"291-302","source":"Crossref","is-referenced-by-count":147,"title":["Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows"],"prefix":"10.3168","volume":"94","author":[{"given":"T.M.A.","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.O.","family":"Gilbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.C.","family":"Bicalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1747","reference":[{"key":"10.3168\/jds.2010-3668_bib0005","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.vetmic.2004.02.012","article-title":"Antimicrobial susceptibility of Haemophilus parasuis and Histophilus somni from pigs and cattle in Denmark","volume":"101","author":"Aarestrup","year":"2004","journal-title":"Vet. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0010","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.3168\/jds.2010-3668_bib0015","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1128\/MMBR.59.1.143-169.1995","article-title":"Phylogenetic identification and in situ detection of individual microbial cells without cultivation","volume":"59","author":"Amann","year":"1995","journal-title":"Microbiol. Rev."},{"key":"10.3168\/jds.2010-3668_bib0020","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1099\/ijs.0.02637-0","article-title":"Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis \u2018Haemophilus somnus\u2019, \u2018Haemophilus agni\u2019 and \u2018Histophilus ovis\u2019","volume":"53","author":"Angen","year":"2003","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0025","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1099\/00207713-49-1-67","volume":"49","author":"Angen","year":"1999","journal-title":"Int. J. Syst. Bacteriol."},{"key":"10.3168\/jds.2010-3668_bib0030","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.anireprosci.2008.01.010","article-title":"Postpartum uterine infection in cattle","volume":"105","author":"Azawi","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2010-3668_bib0035","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1751-0813.2002.tb12058.x","article-title":"Phenotypic characterisation of australian sheep and cattle isolates of Mannheimia haemolytica, Mannheimia granulomatis and Mannheimiavarigiena","volume":"80","author":"Blackall","year":"2002","journal-title":"Aust. Vet. J."},{"key":"10.3168\/jds.2010-3668_bib0040","doi-asserted-by":"crossref","first-page":"6193","DOI":"10.1128\/AEM.71.10.6193-6198.2005","article-title":"Phylum- and class-specific PCR primers for general microbial community analysis","volume":"71","author":"Blackwood","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0045","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.theriogenology.2007.05.040","article-title":"Sample-based assessment of the microbial etiology of bovine necrotic vulvovaginitis","volume":"68","author":"Blum","year":"2007","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2010-3668_bib0050","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s002030000248","article-title":"Analysis of a 2,4,6-trichlorophenol-dehalogenating enrichment culture and isolation of the dehalogenating member Desulfitobacterium frappieri strain TCP-A","volume":"175","author":"Breitenstein","year":"2001","journal-title":"Arch. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0055","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0022-2836(81)90508-8","article-title":"Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli","volume":"148","author":"Brosius","year":"1981","journal-title":"J. Mol. Biol."},{"key":"10.3168\/jds.2010-3668_bib0060","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.rvsc.2004.04.002","article-title":"Fatal meningitis in a calf caused by Mannheimia varigena","volume":"77","author":"Catry","year":"2004","journal-title":"Res. Vet. Sci."},{"key":"10.3168\/jds.2010-3668_bib0065","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/j.0006-341X.2002.00531.x","article-title":"Estimating the number of species in a stochastic abundance model","volume":"58","author":"Chao","year":"2002","journal-title":"Biometrics"},{"key":"10.3168\/jds.2010-3668_bib0070","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1098\/rstb.1994.0091","article-title":"Estimating terrestrial biodiversity through extrapolation","volume":"345","author":"Colwell","year":"1994","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.3168\/jds.2010-3668_bib0075","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1126\/science.1120250","article-title":"Community genomics among stratified microbial assemblages in the ocean's interior","volume":"311","author":"DeLong","year":"2006","journal-title":"Science"},{"key":"10.3168\/jds.2010-3668_bib0080","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/S0093-691X(00)00410-6","article-title":"Relationship between intra-uterine bacterial contamination, endotoxin levels and the development of endometritis in postpartum cows with dystocia or retained placenta","volume":"54","author":"Dohmen","year":"2000","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2010-3668_bib0085","first-page":"259","article-title":"Experimental bovine genital ureaplasmosis. II. Granular vulvitis, endometritis and salpingitis following uterine inoculation","volume":"44","author":"Doig","year":"1980","journal-title":"Can. J. Comp. Med."},{"key":"10.3168\/jds.2010-3668_bib0090","doi-asserted-by":"crossref","first-page":"505","DOI":"10.3201\/eid1003.020592","article-title":"Bovine necrotic vulvovaginitis associated with Porphyromonas levii","volume":"10","author":"Elad","year":"2004","journal-title":"Emerg. Infect. Dis."},{"key":"10.3168\/jds.2010-3668_bib0095","first-page":"91","article-title":"Compositional shifts of bacterial groups in a solarized and amended soil as determined by denaturing gradient gel electrophoresis","volume":"38","author":"Gelsomino","year":"2006","journal-title":"Plant Soil"},{"key":"10.3168\/jds.2010-3668_bib0100","series-title":"Sequencing and Hybridization Techniques in Bacterial Systematics","first-page":"177","article-title":"The polymerase chain reaction","author":"Giovannoni","year":"1991"},{"key":"10.3168\/jds.2010-3668_bib0105","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1023\/A:1010350406708","article-title":"Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis","volume":"232","author":"Gomes","year":"2001","journal-title":"Plant Soil"},{"key":"10.3168\/jds.2010-3668_bib0110","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/419575a","article-title":"Ecology: Biodiversity in the scales","volume":"419","author":"Gotelli","year":"2002","journal-title":"Nature"},{"key":"10.3168\/jds.2010-3668_bib0115","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1128\/MMBR.68.4.669-685.2004","article-title":"Metagenomics: Application of genomics to uncultured microorganisms","volume":"68","author":"Handelsman","year":"2004","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.3168\/jds.2010-3668_bib0120","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1128\/AEM.63.8.3233-3241.1997","article-title":"Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients","volume":"63","author":"Heuer","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0125","first-page":"21","article-title":"Evolution of protein molecules","volume":"Vol. 3","author":"Jukes","year":"1969"},{"key":"10.3168\/jds.2010-3668_bib0130","first-page":"440","article-title":"The effect of intrauterine inoculation with Ureaplasma diversum on bovine fertility","volume":"51","author":"Kreplin","year":"1987","journal-title":"Can. J. Vet. Res."},{"key":"10.3168\/jds.2010-3668_bib0135","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"10.3168\/jds.2010-3668_bib0140","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1016\/j.theriogenology.2007.07.013","article-title":"The effects of Arcanobacterium pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo","volume":"68","author":"Miller","year":"2007","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2010-3668_bib0145","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1038\/ismej.2007.97","article-title":"Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities","volume":"2","author":"M\u00fchling","year":"2008","journal-title":"ISME J."},{"key":"10.3168\/jds.2010-3668_bib0150","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/AEM.59.3.695-700.1993","article-title":"Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA","volume":"59","author":"Muyzer","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0155","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1128\/jb.178.19.5636-5643.1996","article-title":"Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis","volume":"178","author":"N\u00fcbel","year":"1996","journal-title":"J. Bacteriol."},{"key":"10.3168\/jds.2010-3668_bib0160","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.joen.2005.07.004","article-title":"Culture-independent detection of Eikenella corrodens and Veillonella parvula in primary endodontic infections","volume":"32","author":"R\u00f4\u00e7as","year":"2006","journal-title":"J. Endod."},{"key":"10.3168\/jds.2010-3668_bib0165","doi-asserted-by":"crossref","first-page":"3599","DOI":"10.1128\/JCM.00431-08","article-title":"Root canal microbiota of teeth with chronic apical periodontitis","volume":"46","author":"R\u00f4\u00e7as","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0170","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1128\/AEM.66.6.2541-2547.2000","article-title":"Cloning the soil metagenome: A strategy for accessing the genetic and functional diversity of uncultured microorganisms","volume":"66","author":"Rondon","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"10.3168\/jds.2010-3668_bib0175","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"10.3168\/jds.2010-3668_bib0180","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"10.3168\/jds.2010-3668_bib0185","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1136\/vr.155.13.383","article-title":"Minimum inhibitory concentrations of some antimicrobial drugs against bacteria causing uterine infections in cattle","volume":"155","author":"Sheldon","year":"2004","journal-title":"Vet. Rec."},{"key":"10.3168\/jds.2010-3668_bib0190","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1016\/j.theriogenology.2005.08.021","article-title":"Defining postpartum uterine disease in cattle","volume":"65","author":"Sheldon","year":"2006","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2010-3668_bib0195","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1530\/rep.0.1230837","article-title":"Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle","volume":"123","author":"Sheldon","year":"2002","journal-title":"Reproduction"},{"key":"10.3168\/jds.2010-3668_bib0200","doi-asserted-by":"crossref","first-page":"e9192","DOI":"10.1371\/journal.pone.0009192","article-title":"Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice","volume":"5","author":"Sheldon","year":"2010","journal-title":"PLoS ONE"},{"key":"10.3168\/jds.2010-3668_bib0205","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007","journal-title":"Mol. Biol. Evol."},{"key":"10.3168\/jds.2010-3668_bib0210","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nature07540","article-title":"A core gut microbiome in obese and lean twins","volume":"457","author":"Turnbaugh","year":"2009","journal-title":"Nature"},{"key":"10.3168\/jds.2010-3668_bib0215","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1128\/MMBR.60.2.407-438.1996","article-title":"Polyphasic taxonomy, a consensus approach to bacterial systematics","volume":"60","author":"Vandamme","year":"1996","journal-title":"Microbiol. Rev."},{"key":"10.3168\/jds.2010-3668_bib0220","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/jb.173.2.697-703.1991","article-title":"16S ribosomal DNA amplification for phylogenetic study","volume":"173","author":"Weisburg","year":"1991","journal-title":"J. Bacteriol."},{"key":"10.3168\/jds.2010-3668_bib0225","doi-asserted-by":"crossref","first-page":"6578","DOI":"10.1073\/pnas.95.12.6578","article-title":"Prokaryotes: The unseen majority","volume":"95","author":"Whitman","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.3168\/jds.2010-3668_bib0230","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.theriogenology.2007.04.056","article-title":"The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow","volume":"68","author":"Williams","year":"2007","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2010-3668_bib0235","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.theriogenology.2004.03.017","article-title":"Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle","volume":"63","author":"Williams","year":"2005","journal-title":"Theriogenology"}],"container-title":["Journal of Dairy Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002203021000696X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002203021000696X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002203021000696X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T21:42:33Z","timestamp":1731102153000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002203021000696X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["S002203021000696X"],"URL":"https:\/\/doi.org\/10.3168\/jds.2010-3668","relation":{},"ISSN":["0022-0302"],"issn-type":[{"value":"0022-0302","type":"print"}],"subject":[],"published":{"date-parts":[[2011,1]]}}}