{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T18:20:05Z","timestamp":1776277205941,"version":"3.50.1"},"reference-count":49,"publisher":"American Dairy Science Association","issue":"2","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31571852"],"award-info":[{"award-number":["31571852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31471598"],"award-info":[{"award-number":["31471598"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31671869"],"award-info":[{"award-number":["31671869"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31601487"],"award-info":[{"award-number":["31601487"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"crossref","award":["BK20151544"],"award-info":[{"award-number":["BK20151544"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Foundation of Jiangsu Province","award":["BRA2017450"],"award-info":[{"award-number":["BRA2017450"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Science"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.3168\/jds.2018-14899","type":"journal-article","created":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T22:31:18Z","timestamp":1545863478000},"page":"961-975","source":"Crossref","is-referenced-by-count":38,"title":["Production and transepithelial transportation of angiotensin-I-converting enzyme (ACE)-inhibitory peptides from whey protein hydrolyzed by immobilized Lactobacillus helveticus proteinase"],"prefix":"10.3168","volume":"102","author":[{"given":"Yuxing","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binyi","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqun","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangying","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daodong","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1747","reference":[{"key":"10.3168\/jds.2018-14899_bib1","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.3168\/jds.S0022-0302(98)75878-3","article-title":"Structural analysis of new antihypertensive peptides derived from cheese whey protein by proteinase K digestion","volume":"81","author":"Abubakar","year":"1998","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-14899_bib2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0169-409X(96)00415-2","article-title":"Caco-2 monolayers in experimental and theoretical predictions of drug transport","volume":"22","author":"Artursson","year":"1996","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.3168\/jds.2018-14899_bib3","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation on microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.3168\/jds.2018-14899_bib4","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1016\/0006-2952(71)90292-9","article-title":"Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung","volume":"20","author":"Cushman","year":"1971","journal-title":"Biochem. Pharmacol."},{"key":"10.3168\/jds.2018-14899_bib5","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.canlet.2005.07.007","article-title":"Modulatory effects of quercetin on proliferation and differentiation of the human colorectal cell line Caco-2","volume":"238","author":"Dihal","year":"2006","journal-title":"Cancer Lett."},{"key":"10.3168\/jds.2018-14899_bib6","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.idairyj.2013.05.009","article-title":"Bioavailability of antihypertensive lactoferricin B-derived peptides: Transepithelial transport and resistance to intestinal and plasma peptidases","volume":"32","author":"Fern\u00e1ndez-Musoles","year":"2013","journal-title":"Int. Dairy J."},{"key":"10.3168\/jds.2018-14899_bib7","doi-asserted-by":"crossref","first-page":"e12269","DOI":"10.14814\/phy2.12269","article-title":"An assessment of human gastric fluid composition as a function of PPI usage","volume":"3","author":"Foltz","year":"2015","journal-title":"Physiol. Rep."},{"key":"10.3168\/jds.2018-14899_bib8","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.jff.2016.03.026","article-title":"Revalorisation of bovine collagen as a potential precursor of angiotensin I-converting enzyme (ACE) inhibitory peptides based on in silico and in vitro protein digestions","volume":"24","author":"Fu","year":"2016","journal-title":"J. Funct. Foods"},{"key":"10.3168\/jds.2018-14899_bib9","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1016\/j.procbio.2014.01.026","article-title":"Immobilization of fructosyltransferase by chitosan and alginate for efficient production of fructooligosaccharides","volume":"49","author":"Ganaie","year":"2014","journal-title":"Process Biochem."},{"key":"10.3168\/jds.2018-14899_bib10","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1002\/jsfa.8545","article-title":"Transepithelial transport of milk-derived angiotensin-I converting enzyme inhibitory peptide with the RLSFNP sequence","volume":"98","author":"Guo","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"10.3168\/jds.2018-14899_bib11","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.foodchem.2008.09.041","article-title":"Optimisation of hydrolysis conditions for the production of the angiotensin-I converting enzyme (ACE) inhibitory peptides from whey protein using response surface methodology","volume":"114","author":"Guo","year":"2009","journal-title":"Food Chem."},{"key":"10.3168\/jds.2018-14899_bib12","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.idairyj.2013.11.004","article-title":"Dairy protein hydrolysates: Peptides for health benefits","volume":"38","author":"Hern\u00e1ndez-Ledesma","year":"2014","journal-title":"Int. Dairy J."},{"key":"10.3168\/jds.2018-14899_bib13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.cis.2010.11.001","article-title":"Antihypertensive peptides: Production, bioavailability and incorporation into foods","volume":"165","author":"Hern\u00e1ndez-Ledesma","year":"2011","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.3168\/jds.2018-14899_bib14","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/j.procbio.2005.12.002","article-title":"Effect of sodium alginate concentration, bead diameter, initial pH and temperature on lactic acid production from pineapple waste using immobilized Lactobacillus delbrueckii.","volume":"41","author":"Idris","year":"2006","journal-title":"Process Biochem."},{"issue":"Suppl. 2","key":"10.3168\/jds.2018-14899_bib15","first-page":"3520","article-title":"Optimization of lipase entrapment in alginate gel bead for palm olein hydrolysis","volume":"29","author":"Jeamjounkhaw","year":"2007","journal-title":"Songklanakarin J. Sci. Technol."},{"key":"10.3168\/jds.2018-14899_bib16","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ijbiomac.2017.01.104","article-title":"Improved enzyme properties upon glutaraldehyde cross-linking of alginate entrapped xylanase from Bacillus licheniformis","volume":"98","author":"Kumar","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"issue":"Pt. 2","key":"10.3168\/jds.2018-14899_bib17","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1042\/BA20030054","article-title":"Modified alginate matrices for the immobilization of bioactive agents","volume":"39","author":"LeTien","year":"2004","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.3168\/jds.2018-14899_bib18","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.3168\/jds.S0022-0302(96)76487-1","article-title":"Identification of an antihypertensive peptide from casein hydrolysate produced by a proteinase from Lactobacillus helveticus CP790","volume":"79","author":"Maeno","year":"1996","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-14899_bib19","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0301-6226(97)00083-3","article-title":"Biochemical properties of bioactive peptides derived from milk proteins: potential nutraceuticals for food and pharmaceutical applications","volume":"50","author":"Meisel","year":"1997","journal-title":"Livest. Prod. Sci."},{"key":"10.3168\/jds.2018-14899_bib20","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.foodchem.2006.11.016","article-title":"ACE-inhibitory and vasodilator properties of an egg white hydrolysate: Effect of a simulated intestinal digestion","volume":"104","author":"Miguela","year":"2007","journal-title":"Food Chem."},{"key":"10.3168\/jds.2018-14899_bib21","doi-asserted-by":"crossref","first-page":"3394","DOI":"10.1093\/jn\/132.11.3394","article-title":"Melibiose, difructose anhydride III and difructose anhydride IV enhance net calcium absorption in rat small and large intestinal epithelium by increasing the passage of tight junctions in vitro","volume":"132","author":"Mineo","year":"2002","journal-title":"J. Nutr."},{"key":"10.3168\/jds.2018-14899_bib22","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.3168\/jds.S0022-0302(04)70013-2","article-title":"Structural analysis of a new anti-hypertensive peptide (\u03b2-Lactosin B) isolated from a commercial whey product","volume":"87","author":"Murakami","year":"2004","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-14899_bib23","doi-asserted-by":"crossref","first-page":"777","DOI":"10.3168\/jds.S0022-0302(95)76689-9","article-title":"Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milk","volume":"78","author":"Nakamura","year":"1995","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-14899_bib24","first-page":"250","article-title":"Continuous degradation of maltose by enzyme entrapment technology using calcium alginate beads as a matrix","volume":"4","author":"Nawaz","year":"2015","journal-title":"Biochem. Biophys. Rep."},{"key":"10.3168\/jds.2018-14899_bib25","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1016\/j.procbio.2011.02.024","article-title":"Covalent immobilization of xylanase on glutaraldehyde activated alginate beads using response surface methodology: Characterization of immobilized enzyme","volume":"46","author":"Pal","year":"2011","journal-title":"Process Biochem."},{"key":"10.3168\/jds.2018-14899_bib26","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.idairyj.2010.01.007","article-title":"Optimization of sour milk fermentation for the production of ACE-inhibitory peptides and purification of a novel peptide from whey protein hydrolysate","volume":"20","author":"Pan","year":"2010","journal-title":"Int. Dairy J."},{"key":"10.3168\/jds.2018-14899_bib27","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1111\/j.1745-4514.2010.00440.x","article-title":"Anti-fatigue and antioxidative activities of peptides isolated from milk proteins","volume":"35","author":"Pan","year":"2011","journal-title":"J. Food Biochem."},{"key":"10.3168\/jds.2018-14899_bib28","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1023\/A:1012040425146","article-title":"Effect of size and charge on the passive diffusion of peptides across Caco-2 cell monolayers via the paracellular pathway","volume":"14","author":"Pauletti","year":"1997","journal-title":"Pharm. Res."},{"key":"10.3168\/jds.2018-14899_bib29","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.trac.2013.08.001","article-title":"Protein digestomics: Integrated platforms to study food-protein digestion and derived functional and active peptides","volume":"52","author":"Picariello","year":"2013","journal-title":"Trends Anal. Chem."},{"key":"10.3168\/jds.2018-14899_bib30","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.idairyj.2007.09.006","article-title":"Bioavailability of the antihypertensive peptide LHLPLP: Transepithelial flux of HLPLP","volume":"18","author":"Quir\u00f3s","year":"2008","journal-title":"Int. Dairy J."},{"key":"10.3168\/jds.2018-14899_bib31","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s13594-013-0152-3","article-title":"Health-promoting properties of bioactive peptides derived from milk proteins in infant food: A review","volume":"94","author":"Raikos","year":"2014","journal-title":"Dairy Sci. Technol."},{"key":"10.3168\/jds.2018-14899_bib32","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1557\/PROC-331-179","article-title":"Structural elements which govern the resistance of intestinal tissues to compound transport","volume":"331","author":"Rubasa","year":"1993","journal-title":"MRS Online Proc. Library Archive"},{"key":"10.3168\/jds.2018-14899_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2011.01.039","article-title":"Original features of cell-envelope proteinases of Lactobacillus helveticus. A review","volume":"146","author":"Sadat-Mekmene","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"10.3168\/jds.2018-14899_bib34","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1271\/bbb.66.378","article-title":"Transepithelial transport of the bioactive tripeptide, Val-Pro-Pro, in human intestinal Caco-2 cell monolayers","volume":"66","author":"Satake","year":"2002","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.3168\/jds.2018-14899_bib35","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1007\/s11605-010-1321-9","article-title":"TNF-alpha induces vectorial secretion of IL-8 in Caco-2 cells","volume":"14","author":"Sonnier","year":"2010","journal-title":"J. Gastrointest. Surg."},{"key":"10.3168\/jds.2018-14899_bib36","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1177\/2211068214561025","article-title":"TEER measurement techniques for in vitro barrier model systems","volume":"20","author":"Srinivasan","year":"2015","journal-title":"J. Lab. Autom."},{"key":"10.3168\/jds.2018-14899_bib37","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0378-5173(98)00331-7","article-title":"Use of Caco-2 cells and LC\/MS\/MS to screen a peptide combinatorial library for permeable structures","volume":"177","author":"Stevenson","year":"1999","journal-title":"Int. J. Pharm."},{"key":"10.3168\/jds.2018-14899_bib38","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.foodchem.2014.07.045","article-title":"Occurrence and fate of ACE-inhibitor peptides in cheeses and in their digestates following in vitro static gastrointestinal digestion","volume":"168","author":"Stuknyt\u0117","year":"2015","journal-title":"Food Chem."},{"key":"10.3168\/jds.2018-14899_bib39","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.foodchem.2016.09.183","article-title":"Bioavailability of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from Virgibacillus halodenitrificans SK1-3-7 proteinases hydrolyzed tilapia muscle proteins","volume":"220","author":"Toopcham","year":"2017","journal-title":"Food Chem."},{"key":"10.3168\/jds.2018-14899_bib40","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.jff.2015.01.050","article-title":"Characterization and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from tilapia using Virgibacillus halodenitrificans SK1-3-7 proteinases","volume":"14","author":"Toopcham","year":"2015","journal-title":"J. Funct. Foods"},{"key":"10.3168\/jds.2018-14899_bib41","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s00449-006-0106-8","article-title":"A new method for immobilization of acetylcholinesterase","volume":"30","author":"T\u00fcmt\u00fcrk","year":"2007","journal-title":"Bioprocess Biosyst. Eng."},{"key":"10.3168\/jds.2018-14899_bib42","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/S0958-6946(02)00077-8","article-title":"Optimisation of the angiotensin converting enzyme inhibition by whey protein hydrolysates using response surface methodology","volume":"12","author":"Van der Ven","year":"2002","journal-title":"Int. Dairy J."},{"key":"10.3168\/jds.2018-14899_bib43","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1079\/BJN20041189","article-title":"Bioavailability of angiotensin I converting enzyme inhibitory peptides","volume":"92","author":"Vermeirssen","year":"2004","journal-title":"Br. J. Nutr."},{"key":"10.3168\/jds.2018-14899_bib44","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/psc.371","article-title":"Intestinal transport of the lactokinin Ala-Leu-Pro-Met-His-Ile-Arg through a Caco-2 Bbe monolayer","volume":"8","author":"Vermeirssen","year":"2002","journal-title":"J. Pept. Sci."},{"key":"10.3168\/jds.2018-14899_bib45","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.jbiosc.2012.09.020","article-title":"Comparative analysis of proteolytic enzymes need for processing of antihypertensive peptides between Lactobacillus helveticus CM4 and DPC4571","volume":"115","author":"Wakai","year":"2013","journal-title":"J. Biosci. Bioeng."},{"key":"10.3168\/jds.2018-14899_bib46","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.3168\/jds.S0022-0302(99)75364-6","article-title":"Purification and characterization of an antihypertensive peptide from a yogurt-like product fermented by Lactobacillus helveticus CPN4","volume":"82","author":"Yamamoto","year":"1999","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-14899_bib47","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.procbio.2017.03.026","article-title":"Enzyme-inorganic nanoflowers\/alginate microbeads: An enzyme immobilization system and its potential application","volume":"57","author":"Zhao","year":"2017","journal-title":"Process Biochem."},{"key":"10.3168\/jds.2018-14899_bib48","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.ijbiomac.2010.04.001","article-title":"Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate-chitosan beads","volume":"47","author":"Zhou","year":"2010","journal-title":"Int. J. Biol. Macromol."},{"key":"10.3168\/jds.2018-14899_bib49","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.peptides.2007.11.006","article-title":"Identification of ACE-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell monolayers","volume":"29","author":"Zhu","year":"2008","journal-title":"Peptides"}],"container-title":["Journal of Dairy Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030218311184?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030218311184?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030218311184","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T21:20:28Z","timestamp":1731100828000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030218311184"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["S0022030218311184"],"URL":"https:\/\/doi.org\/10.3168\/jds.2018-14899","relation":{},"ISSN":["0022-0302"],"issn-type":[{"value":"0022-0302","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]}}}