{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T13:48:02Z","timestamp":1768484882547,"version":"3.49.0"},"reference-count":42,"publisher":"American Dairy Science Association","issue":"4","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Science"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.3168\/jds.2018-15783","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T11:32:59Z","timestamp":1550835179000},"page":"3439-3451","source":"Crossref","is-referenced-by-count":18,"title":["Effect of pegbovigrastim administration on the microbiome found in the vagina of cows postpartum"],"prefix":"10.3168","volume":"102","author":[{"given":"K.N.","family":"Galv\u00e3o","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.H.","family":"Higgins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Zinicola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.J.","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Korzec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.C.","family":"Bicalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1747","reference":[{"key":"10.3168\/jds.2018-15783_bib1","doi-asserted-by":"crossref","first-page":"3850","DOI":"10.3168\/jds.2016-12058","article-title":"Genetic and functional analysis of the bovine uterine microbiota. Part I: Metritis versus healthy cows","volume":"100","author":"Bicalho","year":"2017","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib2","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.3168\/jds.2016-11623","article-title":"Dynamics of the microbiota found in the vaginas of dairy cows during the transition period: Associations with uterine diseases and reproductive outcome","volume":"100","author":"Bicalho","year":"2017","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib3","doi-asserted-by":"crossref","first-page":"5818","DOI":"10.3168\/jds.2010-3550","article-title":"Molecular and epidemiological characterization of bovine intrauterine Escherichia coli","volume":"93","author":"Bicalho","year":"2010","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib4","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nmeth.2276","article-title":"Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing","volume":"10","author":"Bokulich","year":"2013","journal-title":"Nat. Methods"},{"key":"10.3168\/jds.2018-15783_bib5","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"10.3168\/jds.2018-15783_bib6","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1038\/ismej.2012.8","article-title":"Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms","volume":"6","author":"Caporaso","year":"2012","journal-title":"ISME J."},{"key":"10.3168\/jds.2018-15783_bib7","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.2527\/jas.2009-1781","article-title":"The environmental impact of dairy production: 1944 compared with 2007","volume":"87","author":"Capper","year":"2009","journal-title":"J. Anim. Sci."},{"key":"10.3168\/jds.2018-15783_bib8","doi-asserted-by":"crossref","first-page":"4476","DOI":"10.3168\/jds.2010-4123","article-title":"The cost and management of different types of clinical mastitis in dairy cows estimated by dynamic programming","volume":"94","author":"Cha","year":"2011","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib9","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.mimet.2007.02.005","article-title":"A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria","volume":"69","author":"Chakravorty","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"10.3168\/jds.2018-15783_bib10","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.theriogenology.2018.03.016","article-title":"Quantifying known and emerging uterine pathogens, and evaluating their association with metritis and fever in dairy cows","volume":"114","author":"Cunha","year":"2018","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2018-15783_bib11","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"10.3168\/jds.2018-15783_bib12","doi-asserted-by":"crossref","first-page":"577","DOI":"10.3906\/vet-1407-29","article-title":"Recent advances in the immunology and uterine microbiology of healthy cows and cows that develop uterine disease","volume":"38","author":"Galv\u00e3o","year":"2014","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"10.3168\/jds.2018-15783_bib13","doi-asserted-by":"crossref","first-page":"243","DOI":"10.4056\/sigs.1433550","article-title":"Meeting report: The terabase metagenomics workshop and the vision of an Earth microbiome project","volume":"3","author":"Gilbert","year":"2010","journal-title":"Stand. Genomic Sci."},{"key":"10.3168\/jds.2018-15783_bib14","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.3168\/jds.S0022-0302(97)76055-7","article-title":"Physiological changes at parturition and their relationship to metabolic disorders","volume":"80","author":"Goff","year":"1997","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib15","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1016\/j.theriogenology.2006.07.017","article-title":"Evaluation of intrauterine antibiotic treatment of clinical metritis and retained fetal membranes in dairy cows","volume":"66","author":"Goshen","year":"2006","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2018-15783_bib16","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1056\/NEJM198911233212106","article-title":"Hematopoietic growth factors. Biology and clinical applications","volume":"321","author":"Groopman","year":"1989","journal-title":"N. Engl. J. Med."},{"key":"10.3168\/jds.2018-15783_bib17","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetimm.2006.03.022","article-title":"Neutrophil function and energy status in Holstein cows with uterine health disorders","volume":"113","author":"Hammon","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.3168\/jds.2018-15783_bib18","doi-asserted-by":"crossref","first-page":"231","DOI":"10.2460\/ajvr.76.3.231","article-title":"Efficacy of polyethylene glycol-conjugated bovine granulocyte colony-stimulating factor for reducing the incidence of naturally occurring clinical mastitis in periparturient dairy cows and heifers","volume":"76","author":"Hassfurther","year":"2015","journal-title":"Am. J. Vet. Res."},{"key":"10.3168\/jds.2018-15783_bib19","first-page":"641","article-title":"Bovine uterine defense mechanism: A review","volume":"36","author":"Hussain","year":"1989","journal-title":"Zentralbl. Veterinarmed. B"},{"key":"10.3168\/jds.2018-15783_bib20","doi-asserted-by":"crossref","first-page":"e0165740","DOI":"10.1371\/journal.pone.0165740","article-title":"Uterine microbiota and immune parameters associated with fever in dairy cows with metritis","volume":"11","author":"Jeon","year":"2016","journal-title":"PLoS One"},{"key":"10.3168\/jds.2018-15783_bib21","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.vetmic.2017.12.022","article-title":"Shift of uterine microbiota associated with antibiotic treatment and cure of metritis in dairy cows","volume":"214","author":"Jeon","year":"2018","journal-title":"Vet. Microbiol."},{"key":"10.3168\/jds.2018-15783_bib22","doi-asserted-by":"crossref","first-page":"6324","DOI":"10.1128\/AEM.01753-15","article-title":"Uterine microbiota progression from calving until establishment of metritis in dairy cows","volume":"81","author":"Jeon","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.3168\/jds.2018-15783_bib23","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.3168\/jds.2013-7242","article-title":"Effect of recombinant bovine granulocyte colony-stimulating factor covalently bound to polyethylene glycol injection on neutrophil number and function in periparturient dairy cows","volume":"97","author":"Kimura","year":"2014","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib24","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.theriogenology.2016.02.016","article-title":"An investigation of the microbiota in uterine flush samples and endometrial biopsies from dairy cows during the first 7 weeks postpartum","volume":"86","author":"Knudsen","year":"2016","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2018-15783_bib25","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.3168\/jds.S0022-0302(02)74302-6","article-title":"Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows","volume":"85","author":"LeBlanc","year":"2002","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib26","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.vetmic.2012.04.033","article-title":"Investigation of postpartum dairy cows' uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene","volume":"159","author":"Machado","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.3168\/jds.2018-15783_bib27","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1038\/ismej.2011.139","article-title":"An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea","volume":"6","author":"McDonald","year":"2012","journal-title":"ISME J."},{"key":"10.3168\/jds.2018-15783_bib28","series-title":"Nutrient Requirements of Dairy Cattle","author":"NRC","year":"2001"},{"key":"10.3168\/jds.2018-15783_bib29","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.3168\/jds.S0022-0302(89)79490-X","article-title":"Effects of recombinant granulocyte colony-stimulating factor on Staphylococcus aureus mastitis in lactating dairy cows","volume":"72","author":"Nickerson","year":"1989","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib30","doi-asserted-by":"crossref","first-page":"9017","DOI":"10.1016\/S0021-9258(18)32158-6","article-title":"Purification of a factor inducing differentiation in murine myelomonocytic leukemia cells. Identification as granulocyte colony-stimulating factor","volume":"258","author":"Nicola","year":"1983","journal-title":"J. Biol. Chem."},{"key":"10.3168\/jds.2018-15783_bib31","series-title":"Proc. Dairy Cattle Reproduction Council (DCRC) Convention, Omaha, NE","first-page":"39","article-title":"Economics of postpartum uterine health","author":"Overton","year":"2008"},{"key":"10.3168\/jds.2018-15783_bib32","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1051\/vetres:2003024","article-title":"The bovine neutrophil: Structure and function in blood and milk","volume":"34","author":"Paape","year":"2003","journal-title":"Vet. Res."},{"key":"10.3168\/jds.2018-15783_bib33","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.3168\/jds.2016-12003","article-title":"Investigation of the effect of pegbovigrastim on some periparturient immune disorders and performance in Mexican dairy herds","volume":"100","author":"Ruiz","year":"2017","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib34","first-page":"387","article-title":"Applying nutrition and physiology to improve reproduction in dairy cattle","volume":"67","author":"Santos","year":"2010","journal-title":"Soc. Reprod. Fertil. Suppl."},{"key":"10.3168\/jds.2018-15783_bib35","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1095\/biolreprod.109.077370","article-title":"Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle","volume":"81","author":"Sheldon","year":"2009","journal-title":"Biol. Reprod."},{"key":"10.3168\/jds.2018-15783_bib36","doi-asserted-by":"crossref","first-page":"e9192","DOI":"10.1371\/journal.pone.0009192","article-title":"Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice","volume":"5","author":"Sheldon","year":"2010","journal-title":"PLoS One"},{"key":"10.3168\/jds.2018-15783_bib37","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/s13567-018-0570-6","article-title":"Microbial communities and inflammatory response in the endometrium differ between normal and metritic dairy cows at 5\u201310 days post-partum","volume":"49","author":"Sicsic","year":"2018","journal-title":"Vet. Res."},{"key":"10.3168\/jds.2018-15783_bib38","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1126\/science.232.4746.61","article-title":"Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells","volume":"232","author":"Souza","year":"1986","journal-title":"Science"},{"key":"10.3168\/jds.2018-15783_bib39","author":"USDA-NASS"},{"key":"10.3168\/jds.2018-15783_bib40","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.theriogenology.2004.03.017","article-title":"Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle","volume":"63","author":"Williams","year":"2005","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2018-15783_bib41","doi-asserted-by":"crossref","first-page":"11199","DOI":"10.3168\/jds.2018-14869","article-title":"Effects of pegbovigrastim administration on periparturient diseases, milk production, and reproductive performance of Holstein cows","volume":"101","author":"Zinicola","year":"2018","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2018-15783_bib42","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0171-2985(86)80097-3","article-title":"Recombinant human granulocyte colony stimulating factor: Molecular and biological characterization","volume":"172","author":"Zsebo","year":"1986","journal-title":"Immunobiology"}],"container-title":["Journal of Dairy Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030219301742?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030219301742?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030219301742","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T21:23:42Z","timestamp":1731101022000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030219301742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["S0022030219301742"],"URL":"https:\/\/doi.org\/10.3168\/jds.2018-15783","relation":{},"ISSN":["0022-0302"],"issn-type":[{"value":"0022-0302","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4]]}}}