{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:13:59Z","timestamp":1788884039806,"version":"build-2803163510"},"reference-count":70,"publisher":"American Dairy Science Association","issue":"12","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Science"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.3168\/jds.2019-16572","type":"journal-article","created":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T21:15:33Z","timestamp":1568236533000},"page":"11652-11669","source":"Crossref","is-referenced-by-count":43,"title":["Multicolor flow cytometric analysis of cryopreserved bovine sperm: A tool for the evaluation of bull fertility"],"prefix":"10.3168","volume":"102","author":[{"given":"K.","family":"Bucher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1433-7336","authenticated-orcid":false,"given":"E.","family":"Malama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Siuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Janett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Bollwein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1747","reference":[{"key":"10.3168\/jds.2019-16572_bib1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.theriogenology.2014.09.004","article-title":"Desalted and lyophilized bovine seminal plasma delays induction of the acrosome reaction in frozen-thawed bovine spermatozoa in response to calcium ionophore","volume":"83","author":"Almadaly","year":"2015","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib2","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.theriogenology.2011.09.002","article-title":"Impact of genomic selection of AI dairy sires on their likely utilization and methods to estimate fertility: A paradigm shift","volume":"77","author":"Amann","year":"2012","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib3","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/j.1939-4640.1993.tb03247.x","article-title":"In vitro evaluation of sperm quality: An opinion","volume":"14","author":"Amann","year":"1993","journal-title":"J. Androl."},{"key":"10.3168\/jds.2019-16572_bib4","doi-asserted-by":"crossref","DOI":"10.1530\/REP-13-0178","article-title":"Mitochondria functionality and sperm quality","volume":"146","author":"Amaral","year":"2013","journal-title":"Reproduction"},{"key":"10.3168\/jds.2019-16572_bib5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bbabio.2008.10.003","article-title":"Mitochondrial inhibitors activate influx of external Ca2+ in sea urchin sperm","volume":"1787","author":"Ard\u00f3n","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"10.3168\/jds.2019-16572_bib6","doi-asserted-by":"crossref","first-page":"45","DOI":"10.4141\/cjas94-007","article-title":"Cryopreservation alters the Ca2+ flux of bovine spermatozoa","volume":"74","author":"Bailey","year":"1994","journal-title":"Can. J. Anim. Sci."},{"key":"10.3168\/jds.2019-16572_bib7","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.fertnstert.2005.06.046","article-title":"Mitochondrial membrane potential integrity and plasma membrane translocation of phosphatidylserine as early apoptotic markers: A comparison of two different sperm subpopulations","volume":"85","author":"Barroso","year":"2006","journal-title":"Fertil. Steril."},{"key":"10.3168\/jds.2019-16572_bib8","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1016\/j.theriogenology.2009.10.010","article-title":"In vitro induction of the acrosome reaction in bull sperm and the relationship to field fertility using low-dose inseminations","volume":"73","author":"Birck","year":"2010","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib9","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1439-0531.2007.00876.x","article-title":"Interrelationship between plasma membrane integrity, mitochondrial membrane potential and DNA fragmentation in cryopreserved bovine spermatozoa","volume":"43","author":"Bollwein","year":"2008","journal-title":"Reprod. Domest. Anim."},{"key":"10.3168\/jds.2019-16572_bib10","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1002\/widm.1072","article-title":"Overview of random forest methodology and practical guidance with emphasis on computational biology and bioinformatics","volume":"2","author":"Boulesteix","year":"2012","journal-title":"Wiley Interdiscip. Rev. Data Min. Knowl. Discov."},{"key":"10.3168\/jds.2019-16572_bib11","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1023\/A:1010933404324","article-title":"Random forests","volume":"45","author":"Breiman","year":"2001","journal-title":"Mach. Learn."},{"key":"10.3168\/jds.2019-16572_bib12","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0303-7207(01)00704-3","article-title":"Intracellular calcium regulation in sperm capacitation and acrosomal reaction","volume":"187","author":"Breitbart","year":"2002","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.3168\/jds.2019-16572_bib13","series-title":"Reprod. Domest. Anim. 52(S3) Proceedings of the 21st Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), Bern, Switzerland","first-page":"75","article-title":"Use of a multi- color flow cytometric assay for the evaluation of bull fertility","author":"Bucher","year":"2017"},{"key":"10.3168\/jds.2019-16572_bib14","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/j.1939-4640.2005.tb02878.x","article-title":"Relationship between sperm viability as determined by flow cytometry and nonreturn rate of dairy bulls","volume":"26","author":"Christensen","year":"2005","journal-title":"J. Androl."},{"key":"10.3168\/jds.2019-16572_bib15","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.3168\/jds.2010-3087","article-title":"Relationship among seminal quality measures and field fertility of young dairy bulls using low-dose inseminations","volume":"94","author":"Christensen","year":"2011","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2019-16572_bib16","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1002\/j.1939-4640.2000.tb03425.x","article-title":"Sperm calcium levels and chlortetracycline fluorescence patterns are related to the in vivo fertility of cryopreserved bovine semen","volume":"21","author":"Collin","year":"2000","journal-title":"J. Androl."},{"key":"10.3168\/jds.2019-16572_bib17","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1095\/biolreprod61.5.1226","article-title":"Intracellular Ca2+-Mg2+-ATPase regulates calcium in\ufb02ux and acrosomal exocytosis in bull and ram spermatozoa","volume":"61","author":"Dragileva","year":"1999","journal-title":"Biol. Reprod."},{"key":"10.3168\/jds.2019-16572_bib18","series-title":"Sperm chromatin structure assay for fertility assessment. Current Protocols in Cytometry 13:7.13.1\u20137.13.27","author":"Evenson","year":"2001"},{"key":"10.3168\/jds.2019-16572_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.anireprosci.2016.01.017","article-title":"The Sperm Chromatin Structure Assay (SCSA) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility","volume":"169","author":"Evenson","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib20","doi-asserted-by":"crossref","first-page":"176","DOI":"10.2164\/jandrol.04152","article-title":"DNA damage in bovine sperm does not block fertilization and early embryonic development but induces apoptosis after the first cleavages","volume":"27","author":"Fatehi","year":"2006","journal-title":"J. Androl."},{"key":"10.3168\/jds.2019-16572_bib21","series-title":"CEUR Workshop Proceedings, Dublin, Ireland","first-page":"57","article-title":"A comparison of machine learning techniques for predicting insemination outcome in Irish dairy cows","author":"Fenlon","year":"2016"},{"key":"10.3168\/jds.2019-16572_bib22","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.urology.2011.12.042","article-title":"Mitochondrial respiratory efficiency is positively correlated with human sperm motility","volume":"79","author":"Ferramosca","year":"2012","journal-title":"Urology"},{"key":"10.3168\/jds.2019-16572_bib23","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/mrd.1080400213","article-title":"Ca2+-Regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis","volume":"40","author":"Fraser","year":"1995","journal-title":"Mol. Reprod. Dev."},{"key":"10.3168\/jds.2019-16572_bib24","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1002\/mrd.22663","article-title":"Chang's meaning of capacitation: A molecular perspective","volume":"83","author":"Gervasi","year":"2016","journal-title":"Mol. Reprod. Dev."},{"key":"10.3168\/jds.2019-16572_bib25","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.anireprosci.2006.12.010","article-title":"Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls","volume":"103","author":"Gillan","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib26","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1017\/S1751731117000684","article-title":"The combination of kinetic and flow cytometric semen parameters as a tool to predict fertility in cryopreserved bull semen","volume":"11","author":"Gliozzi","year":"2017","journal-title":"Animal"},{"key":"10.3168\/jds.2019-16572_bib27","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1016\/j.theriogenology.2005.07.009","article-title":"Usefulness of a triple fluorochrome combination Merocyanine 540\/Yo-Pro 1\/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls","volume":"65","author":"Hallap","year":"2006","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib28","doi-asserted-by":"crossref","first-page":"5262","DOI":"10.3168\/jds.2014-8984","article-title":"Machine learning algorithms for the prediction of conception success to a given insemination in lactating dairy cows","volume":"98","author":"Hempstalk","year":"2015","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2019-16572_bib29","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1016\/j.theriogenology.2008.12.006","article-title":"Can programmed cell death be induced in post-ejaculatory bull and stallion spermatozoa?","volume":"71","author":"Hendricks","year":"2009","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib30","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/S0093-691X(01)00456-3","article-title":"Assessment of sperm quality through fluorometry and sperm chromatin structure assay in relation to field fertility of frozen-thawed semen from Swedish AI bulls","volume":"55","author":"Januskauska","year":"2001","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib31","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/S0093-691X(03)00050-5","article-title":"Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility","volume":"60","author":"Januskauska","year":"2003","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib32","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.anireprosci.2009.02.003","article-title":"Capacitation status of fresh and frozen-thawed buffalo spermatozoa in relation to cholesterol level, membrane fluidity and intracellular calcium","volume":"116","author":"Kadirvel","year":"2009","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib33","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1017\/S0967199415000490","article-title":"Simultaneous evaluation of plasma membrane integrity, acrosomal integrity, and mitochondrial membrane potential in bovine spermatozoa by flow cytometry","volume":"24","author":"Kanno","year":"2016","journal-title":"Zygote"},{"key":"10.3168\/jds.2019-16572_bib34","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.2527\/jas.2011-4492","article-title":"Is sperm DNA fragmentation a good marker for field AI bull fertility?","volume":"90","author":"Karoui","year":"2012","journal-title":"J. Anim. Sci."},{"key":"10.3168\/jds.2019-16572_bib35","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.rbmo.2012.12.005","article-title":"Flow cytometric characterization of apoptosis and chromatin damage in spermatozoa","volume":"26","author":"Kim","year":"2013","journal-title":"Reprod. Biomed. Online"},{"key":"10.3168\/jds.2019-16572_bib36","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1152\/physrev.00013.2006","article-title":"Mitochondrial membrane permeabilization in cell death","volume":"87","author":"Kroemer","year":"2007","journal-title":"Physiol. Rev."},{"key":"10.3168\/jds.2019-16572_bib37","doi-asserted-by":"crossref","first-page":"5824","DOI":"10.3168\/jds.2016-12484","article-title":"Sperm viability, reactive oxygen species, and DNA fragmentation index combined can discriminate between above- and below-average fertility bulls","volume":"100","author":"Kumaresan","year":"2017","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2019-16572_bib38","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.fertnstert.2012.09.021","article-title":"Voltage-dependent anion channels are a key factor of male fertility","volume":"99","author":"Kwon","year":"2013","journal-title":"Fertil. Steril."},{"key":"10.3168\/jds.2019-16572_bib39","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1002\/cyto.a.20623","article-title":"MIFlowCyt: The minimum information about a flow cytometry experiment","volume":"73","author":"Lee","year":"2008","journal-title":"Cytometry A"},{"key":"10.3168\/jds.2019-16572_bib40","first-page":"18","article-title":"Classification and regression by random forest","volume":"2","author":"Liaw","year":"2002","journal-title":"R News"},{"key":"10.3168\/jds.2019-16572_bib41","author":"Malama","year":"2016"},{"key":"10.3168\/jds.2019-16572_bib42","unstructured":"Meier, A. 2017. Risk factor study of pododermatitis in rabbits using additive Bayesian networks. Master Thesis in Biostatistics (STA495). Section of Epidemiology, University of Zurich, Zurich, Switzerland."},{"key":"10.3168\/jds.2019-16572_bib43","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.anireprosci.2011.03.010","article-title":"Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa","volume":"125","author":"Miah","year":"2011","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib44","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1262\/jrd.2016-107","article-title":"Involvement of calcium channels and intracellular calcium in bull sperm thermotaxis","volume":"63","author":"Mondal","year":"2017","journal-title":"J. Reprod. Dev."},{"key":"10.3168\/jds.2019-16572_bib45","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.anireprosci.2017.08.001","article-title":"Sperm quality variables as indicators of bull fertility may be breed dependent","volume":"185","author":"Morrell","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib46","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.anireprosci.2003.08.003","article-title":"Changes in plasma membrane and acrosome integrity of frozen-thawed bovine spermatozoa during a 4 h incubation as measured by multicolor flow cytometry","volume":"80","author":"Nagy","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib47","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1095\/biolreprod.102.011445","article-title":"A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles","volume":"68","author":"Nagy","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.3168\/jds.2019-16572_bib48","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/j.theriogenology.2013.02.011","article-title":"Sperm chromatin structure and sperm morphology: Their association with fertility in AI dairy Ayrshire sires","volume":"79","author":"Nagy","year":"2013","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib49","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.anireprosci.2013.01.010","article-title":"Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed AI program","volume":"137","author":"Oliveira","year":"2013","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib50","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1016\/j.fertnstert.2011.03.059","article-title":"Mitochondrial membrane potential profile and its correlation with increasing sperm motility","volume":"95","author":"Paoli","year":"2011","journal-title":"Fertil. Steril."},{"key":"10.3168\/jds.2019-16572_bib51","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/rda.12202","article-title":"Fluorescence technologies for evaluating male gamete (dys)function","volume":"48","author":"Petrunkina","year":"2013","journal-title":"Reprod. Domest. Anim."},{"key":"10.3168\/jds.2019-16572_bib52","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1002\/cyto.a.21044","article-title":"Cytometric solutions in veterinary andrology: Developments, advantages, and limitations","volume":"79 A","author":"Petrunkina","year":"2011","journal-title":"Cytom. Part A"},{"key":"10.3168\/jds.2019-16572_bib53","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.anireprosci.2003.10.003","article-title":"Relationship between thirty post-thaw spermatozoal characteristics and the field fertility of 11 high-use Australian dairy AI sires","volume":"81","author":"Phillips","year":"2004","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib54","author":"Pinheiro"},{"key":"10.3168\/jds.2019-16572_bib55","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.anireprosci.2012.04.006","article-title":"The usefulness of combining traditional sperm assessments with in vitro heterospermic insemination to identify bulls of low fertility as estimated in vivo","volume":"132","author":"Puglisi","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib56","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/841615","article-title":"Calcium influx and male fertility in the context of the sperm proteome: An update","volume":"2014","author":"Rahman","year":"2014","journal-title":"BioMed Res. Int."},{"key":"10.3168\/jds.2019-16572_bib57","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/35098027","article-title":"A sperm ion channel required for sperm motility and male fertility","volume":"413","author":"Ren","year":"2001","journal-title":"Nature"},{"key":"10.3168\/jds.2019-16572_bib58","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1016\/S0021-9258(17)43388-6","article-title":"Regulation of calcium transport in bovine spermatozoa","volume":"259","author":"Rufo","year":"1984","journal-title":"J. Biol. Chem."},{"key":"10.3168\/jds.2019-16572_bib59","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S0378-4320(00)00137-8","article-title":"Relationship of seminal traits and insemination time to fertilization rate and embryo quality","volume":"60\u201361","author":"Saacke","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib60","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1016\/j.theriogenology.2015.07.035","article-title":"Use of combinations of in vitro quality assessments to predict fertility of bovine semen","volume":"84","author":"Sellem","year":"2015","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib61","doi-asserted-by":"crossref","first-page":"731","DOI":"10.3168\/jds.2013-6693","article-title":"Prediction of insemination outcomes in Holstein dairy cattle using alternative machine learning algorithms","volume":"97","author":"Shahinfar","year":"2014","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2019-16572_bib62","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1530\/REP-13-0123","article-title":"Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome","volume":"146","author":"Sim\u00f5es","year":"2013","journal-title":"Reproduction"},{"key":"10.3168\/jds.2019-16572_bib63","doi-asserted-by":"crossref","first-page":"3940","DOI":"10.1093\/bioinformatics\/bti623","article-title":"ROCR: Visualizing classifier performance in R","volume":"21","author":"Sing","year":"2005","journal-title":"Bioinformatics"},{"key":"10.3168\/jds.2019-16572_bib64","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1016\/j.theriogenology.2004.01.012","article-title":"Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen","volume":"62","author":"Tartaglione","year":"2004","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib65","series-title":"A language and environment for statistical computing","author":"The R Development Core Team","year":"2016"},{"key":"10.3168\/jds.2019-16572_bib66","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/j.theriogenology.2005.08.022","article-title":"Capacitation-like changes in equine spermatozoa following cryopreservation","volume":"65","author":"Thomas","year":"2006","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2019-16572_bib67","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1093\/bib\/bbs034","article-title":"Data mining in the life sciences with random forest: A walk in the park or lost in the jungle?","volume":"14","author":"Touw","year":"2013","journal-title":"Brief. Bioinform."},{"key":"10.3168\/jds.2019-16572_bib68","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.anireprosci.2015.12.011","article-title":"Prediction of bull fertility","volume":"169","author":"Utt","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"10.3168\/jds.2019-16572_bib69","first-page":"153","article-title":"Bovine semen quality control in artificial insemination centers","volume":"9","author":"Vincent","year":"2012","journal-title":"Anim. Reprod."},{"key":"10.3168\/jds.2019-16572_bib70","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ab.2017.07.009","article-title":"Mitochondrial membrane potential","volume":"552","author":"Zorova","year":"2018","journal-title":"Anal. Biochem."}],"container-title":["Journal of Dairy Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030219307994?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030219307994?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030219307994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T21:38:17Z","timestamp":1731101897000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030219307994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":70,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["S0022030219307994"],"URL":"https:\/\/doi.org\/10.3168\/jds.2019-16572","relation":{},"ISSN":["0022-0302"],"issn-type":[{"value":"0022-0302","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12]]}}}