{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:15:05Z","timestamp":1787498105095,"version":"build-2736575974"},"reference-count":58,"publisher":"American Dairy Science Association","issue":"2","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["journalofdairyscience.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Dairy Science"],"published-print":{"date-parts":[[2024,2]]},"DOI":"10.3168\/jds.2023-23736","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T21:18:05Z","timestamp":1695935885000},"page":"1244-1262","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of ruminal lipopolysaccharide exposure on primary bovine ruminal epithelial cells"],"prefix":"10.3168","volume":"107","author":[{"given":"E.","family":"Sarmikasoglou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3079-4282","authenticated-orcid":false,"given":"L.","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0935-6233","authenticated-orcid":false,"given":"A.P.","family":"Faciola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1747","reference":[{"key":"10.3168\/jds.2023-23736_bib1","doi-asserted-by":"crossref","first-page":"464","DOI":"10.2527\/2000.782464x","article-title":"Effect and absorption of histamine in sheep rumen: Significance of acidotic epithelial damage","volume":"78","author":"Aschenbach","year":"2000","journal-title":"J. Anim. Sci."},{"key":"10.3168\/jds.2023-23736_bib2","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.2527\/jas.2010-3301","article-title":"Ruminant nutrition symposium: Role of fermentation acid absorption in the regulation of ruminal pH","volume":"89","author":"Aschenbach","year":"2011","journal-title":"J. Anim. Sci."},{"key":"10.3168\/jds.2023-23736_bib3","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.3168\/jds.2018-15243","article-title":"Symposium review: The importance of the ruminal epithelial barrier for a healthy and productive cow","volume":"102","author":"Aschenbach","year":"2019","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib4","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.vetmic.2012.04.032","article-title":"Variation of bacterial communities and expression of toll-like receptor genes in the rumen of steers differing in susceptibility to subacute ruminal acidosis","volume":"159","author":"Chen","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.3168\/jds.2023-23736_bib5","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3389\/fmicb.2014.00781","article-title":"Intestinal mucosal tolerance and impact of gut microbiota to mucosal tolerance","volume":"5","author":"Chistiakov","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.3168\/jds.2023-23736_bib6","doi-asserted-by":"crossref","DOI":"10.1128\/mSystems.00046-17","article-title":"Total lipopolysaccharide from the human gut microbiome silences toll-like receptor signaling","volume":"2","author":"d\u2019Hennezel","year":"2017","journal-title":"mSystems"},{"key":"10.3168\/jds.2023-23736_bib7","doi-asserted-by":"crossref","first-page":"334","DOI":"10.3168\/jds.2018-14807","article-title":"Effects of lipopolysaccharide dosing on bacterial community composition and fermentation in a dual-flow continuous culture system","volume":"102","author":"Dai","year":"2019","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1365-2567.2006.02344.x","article-title":"Lipopolysaccharide induces CXCL2\/macrophage inflammatory protein-2 gene expression in enterocytes via NF-\u03baB activation: Independence from endogenous TNF-\u03b1 and platelet-activating factor","volume":"118","author":"De Plaen","year":"2006","journal-title":"Immunology"},{"key":"10.3168\/jds.2023-23736_bib9","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3844\/ajavsp.2012.141.148","article-title":"A Characterization of inflammatory and structural markers within the rumen epithelium during grain-induced ruminal acidosis in lactating dairy cattle","volume":"7","author":"Dionissopoulos","year":"2012","journal-title":"Am. J. Anim. Vet. Sci."},{"key":"10.3168\/jds.2023-23736_bib10","doi-asserted-by":"crossref","first-page":"20809","DOI":"10.1074\/jbc.C100140200","article-title":"Non-small cell lung cancer cyclooxygenase-2-dependent invasion is mediated by CD44","volume":"276","author":"Dohadwala","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.3168\/jds.2023-23736_bib11","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.4049\/jimmunol.1202368","article-title":"GM-CSF produced by nonhematopoietic cells is required for early epithelial cell proliferation and repair of injured colonic mucosa","volume":"190","author":"Egea","year":"2013","journal-title":"J. Immunol."},{"key":"10.3168\/jds.2023-23736_bib12","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.cmet.2018.08.005","article-title":"Functional gut microbiota remodeling contributes to the caloric restriction-induced metabolic improvements","volume":"28","author":"Fabbiano","year":"2018","journal-title":"Cell Metab."},{"key":"10.3168\/jds.2023-23736_bib13","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1053\/j.gastro.2006.06.017","article-title":"Cox-2 is regulated by toll-like receptor-4 (TLR4) signaling: Role in proliferation and apoptosis in the intestine","volume":"131","author":"Fukata","year":"2006","journal-title":"Gastroenterology"},{"key":"10.3168\/jds.2023-23736_bib14","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/BF02278507","article-title":"Culture of epithelial cells from bovine ruminal mucosa","volume":"4","author":"G\u00e1lfi","year":"1980","journal-title":"Vet. Res. Commun."},{"key":"10.3168\/jds.2023-23736_bib15","doi-asserted-by":"crossref","first-page":"856","DOI":"10.3168\/jds.S0022-0302(07)71569-2","article-title":"Ruminal lipopolysaccharide concentration and inflammatory response during grain-induced subacute ruminal acidosis in dairy cows","volume":"90","author":"Gozho","year":"2007","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib16","doi-asserted-by":"crossref","first-page":"580","DOI":"10.4049\/jimmunol.176.1.580","article-title":"Lipopolysaccharide induces cyclooxygenase-2 in intestinal epithelium via a noncanonical p38 MAPK pathway","volume":"176","author":"Grishin","year":"2006","journal-title":"J. Immunol."},{"key":"10.3168\/jds.2023-23736_bib17","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1002\/jlb.56.5.559","article-title":"Essential involvement of interleukin-8 (IL-8) in acute inflammation","volume":"56","author":"Harada","year":"1994","journal-title":"J. Leukoc. Biol."},{"key":"10.3168\/jds.2023-23736_bib18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1477-7827-7-55","article-title":"Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility","volume":"7","author":"Herath","year":"2009","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.3168\/jds.2023-23736_bib19","first-page":"2006","article-title":"Cyclooxygenase-2 inhibitor induces apoptosis and enhances cytotoxicity of various anticancer agents in non-small cell lung cancer cell lines","volume":"6","author":"Hida","year":"2000","journal-title":"Clin. Cancer Res."},{"key":"10.3168\/jds.2023-23736_bib20","series-title":"Advances in Immunology","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/bs.ai.2014.11.003","article-title":"Chapter 3 - Epithelial cell contributions to intestinal immunity","author":"Hooper","year":"2015"},{"key":"10.3168\/jds.2023-23736_bib21","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1172\/JCI117676","article-title":"A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion","volume":"95","author":"Jung","year":"1995","journal-title":"J. Clin. Invest."},{"key":"10.3168\/jds.2023-23736_bib22","doi-asserted-by":"crossref","first-page":"9587","DOI":"10.3168\/jds.2020-18652","article-title":"Effects of lipopolysaccharide exposure in primary bovine ruminal epithelial cells","volume":"103","author":"Kent-Dennis","year":"2020","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib23","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.3168\/jds.2020-19092","article-title":"Effects of a proinflammatory response on metabolic function of cultured, primary ruminal epithelial cells","volume":"104","author":"Kent-Dennis","year":"2021","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib24","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.3168\/jds.2008-1389","article-title":"A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation","volume":"92","author":"Khafipour","year":"2009","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib25","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.3168\/jds.2008-1656","article-title":"Alfalfa pellet-induced subacute ruminal acidosis in dairy cows increases bacterial endotoxin in the rumen without causing inflammation","volume":"92","author":"Khafipour","year":"2009","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib26","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1152\/physiolgenomics.00086.2016","article-title":"Effects of dietary forage and calf starter on ruminal pH and transcriptomic adaptation of the rumen epithelium in Holstein calves during the weaning transition","volume":"48","author":"Kim","year":"2016","journal-title":"Physiol. Genomics"},{"key":"10.3168\/jds.2023-23736_bib27","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10616-009-9190-3","article-title":"CD90 expression on human primary cells and elimination of contaminating fibroblasts from cell cultures","volume":"59","author":"Kisselbach","year":"2009","journal-title":"Cytotechnology"},{"key":"10.3168\/jds.2023-23736_bib28","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00281-007-0099-7","article-title":"The \u201cpolarizing\u2013tolerizing\u201d mechanism of intestinal epithelium: Its relevance to colonic homeostasis","volume":"30","author":"Lee","year":"2008","journal-title":"Semin. Immunopathol."},{"key":"10.3168\/jds.2023-23736_bib29","doi-asserted-by":"crossref","first-page":"R232","DOI":"10.1152\/ajpregu.00068.2013","article-title":"A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats","volume":"305","author":"Liu","year":"2013","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"10.3168\/jds.2023-23736_bib30","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/j.1365-2567.2007.02639.x","article-title":"Cytokine-mediated control of lipopolysaccharide-induced activation of small intestinal epithelial cells","volume":"122","author":"Lotz","year":"2007","journal-title":"Immunology"},{"key":"10.3168\/jds.2023-23736_bib31","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.vetimm.2012.01.010","article-title":"Regional and age dependent changes in gene expression of toll-like receptors and key antimicrobial defence molecules throughout the gastrointestinal tract of dairy calves","volume":"146","author":"Malmuthuge","year":"2012","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.3168\/jds.2023-23736_bib32","first-page":"5835","article-title":"Celecoxib modulates the capacity for prostaglandin E2 and interleukin-10 production in alveolar macrophages from active smokers","volume":"9","author":"Mao","year":"2003","journal-title":"Clin. Cancer Res."},{"key":"10.3168\/jds.2023-23736_bib33","doi-asserted-by":"crossref","first-page":"6662","DOI":"10.3168\/jds.2016-12262","article-title":"Key role of short-chain fatty acids in epithelial barrier failure during ruminal acidosis","volume":"100","author":"Meissner","year":"2017","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib34","doi-asserted-by":"crossref","DOI":"10.1126\/science.1249288","article-title":"Microbiota-dependent crosstalk between macrophages and ILC3 promotes intestinal homeostasis","volume":"343","author":"Mortha","year":"2014","journal-title":"Science"},{"key":"10.3168\/jds.2023-23736_bib35","doi-asserted-by":"crossref","first-page":"E17","DOI":"10.3168\/jds.2006-478","article-title":"Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook","volume":"90","author":"Nagaraja","year":"2007","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib36","first-page":"1450","article-title":"Characterization of latent TGF-beta activation by murine peritoneal macrophages","volume":"155","author":"Nunes","year":"1995","journal-title":"J. Immunol. Baltim. Md."},{"key":"10.3168\/jds.2023-23736_bib37","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1136\/gut.49.4.526","article-title":"MIP-2 secreted by epithelial cells increases neutrophil and lymphocyte recruitment in the mouse intestine","volume":"49","author":"Ohtsuka","year":"2001","journal-title":"Gut"},{"key":"10.3168\/jds.2023-23736_bib38","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2527\/1998.761275x","article-title":"Acidosis in cattle: A review","volume":"76","author":"Owens","year":"1998","journal-title":"J. Anim. Sci."},{"key":"10.3168\/jds.2023-23736_bib39","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1038\/nature07830","article-title":"The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex","volume":"458","author":"Park","year":"2009","journal-title":"Nature"},{"key":"10.3168\/jds.2023-23736_bib40","article-title":"Fetal cytokine response to porcine reproductive and respiratory syndrome virus-2 infection","volume":"126","author":"Pasternak","year":"2020","journal-title":"Cytokine"},{"key":"10.3168\/jds.2023-23736_bib41","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.117.005784","article-title":"Gut-derived serum lipopolysaccharide is associated with enhanced risk of major adverse cardiovascular events in atrial fibrillation: Effect of adherence to mediterranean diet","volume":"6","author":"Pastori","year":"2017","journal-title":"J. Am. Heart Assoc."},{"key":"10.3168\/jds.2023-23736_bib42","doi-asserted-by":"crossref","first-page":"s399","DOI":"10.1017\/S1751731118001921","article-title":"Review: Enhancing gastrointestinal health in dairy cows","volume":"12","author":"Plaizier","year":"2018","journal-title":"Animal"},{"key":"10.3168\/jds.2023-23736_bib43","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.anifeedsci.2011.12.004","article-title":"Subacute ruminal acidosis (SARA), endotoxins and health consequences","volume":"172","author":"Plaizier","year":"2012","journal-title":"Anim. Feed Sci. Technol."},{"key":"10.3168\/jds.2023-23736_bib44","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/605959","article-title":"The role of lipids mediators in inflammation and resolution","volume":"2015","author":"Rogerio","year":"2015","journal-title":"BioMed Res. Int."},{"key":"10.3168\/jds.2023-23736_bib45","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/cea.12697","article-title":"Cyclooxygenase 2: Its regulation, role and impact in airway inflammation","volume":"46","author":"Rumzhum","year":"2016","journal-title":"Clin. Exp. Allergy"},{"key":"10.3168\/jds.2023-23736_bib46","author":"Sarmikasoglou"},{"key":"10.3168\/jds.2023-23736_bib47","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-022-20073-2","article-title":"Effects of ruminal lipopolysaccharides on growth and fermentation end products of pure cultured bacteria","volume":"12","author":"Sarmikasoglou","year":"2022","journal-title":"Sci. Rep."},{"key":"10.3168\/jds.2023-23736_bib48","doi-asserted-by":"crossref","first-page":"817","DOI":"10.3390\/polysaccharides2040049","article-title":"Ruminal lipid A analysis by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry","volume":"2","author":"Sarmikasoglou","year":"2021","journal-title":"Polysaccharides"},{"key":"10.3168\/jds.2023-23736_bib49","doi-asserted-by":"crossref","first-page":"5729","DOI":"10.2527\/jas.2013-6471","article-title":"The duration of time that beef cattle are fed a high-grain diet affects the recovery from a bout of ruminal acidosis: Dry matter intake and ruminal fermentation","volume":"91","author":"Schwaiger","year":"2013","journal-title":"J. Anim. Sci."},{"key":"10.3168\/jds.2023-23736_bib50","first-page":"241","article-title":"Intestinal epithelial cells as a source of inflammatory cytokines and chemokines","volume":"16","author":"Stadnyk","year":"2002","journal-title":"Can. J. Gastroenterol. And Hepatol."},{"key":"10.3168\/jds.2023-23736_bib51","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.3168\/jds.2017-12896","article-title":"Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14\/TLR4\/MD2 genes involved in lipopolysaccharide systemic immune response in dairy cows","volume":"101","author":"Stefanska","year":"2018","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2023-23736_bib52","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.ijmm.2016.03.001","article-title":"Structure and function: Lipid A modifications in commensals and pathogens","volume":"306","author":"Steimle","year":"2016","journal-title":"Int. J. Med. Microbiol."},{"key":"10.3168\/jds.2023-23736_bib53","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1016\/j.ymthe.2019.07.007","article-title":"Weak agonistic LPS restores intestinal immune homeostasis","volume":"27","author":"Steimle","year":"2019","journal-title":"Mol. Ther."},{"key":"10.3168\/jds.2023-23736_bib54","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a016295","article-title":"IL-6 in inflammation, immunity, and disease","volume":"6","author":"Tanaka","year":"2014","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.3168\/jds.2023-23736_bib55","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.yexcr.2004.03.007","article-title":"Cytokine-induced upregulation of NF-\u03baB, IL-8, and ICAM-1 is dependent on colonic cell polarity: Implication for PKC\u03b4","volume":"297","author":"Vallee","year":"2004","journal-title":"Exp. Cell Res."},{"key":"10.3168\/jds.2023-23736_bib56","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1016\/j.cell.2016.04.007","article-title":"Variation in microbiome LPS immunogenicity contributes to autoimmunity in humans","volume":"165","author":"Vatanen","year":"2016","journal-title":"Cell"},{"key":"10.3168\/jds.2023-23736_bib57","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1172\/JCI1368","article-title":"IL-6 is an antiinflammatory cytokine required for controlling local or systemic acute inflammatory responses","volume":"101","author":"Xing","year":"1998","journal-title":"J. Clin. Invest."},{"key":"10.3168\/jds.2023-23736_bib58","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s40104-016-0100-1","article-title":"High-concentrate feeding upregulates the expression of inflammation-related genes in the ruminal epithelium of dairy cattle","volume":"7","author":"Zhang","year":"2016","journal-title":"J. Anim. Sci. Biotechnol."}],"container-title":["Journal of Dairy Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030223007051?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030223007051?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030223007051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T16:41:23Z","timestamp":1731084083000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030223007051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":58,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["S0022030223007051"],"URL":"https:\/\/doi.org\/10.3168\/jds.2023-23736","relation":{},"ISSN":["0022-0302"],"issn-type":[{"value":"0022-0302","type":"print"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of ruminal lipopolysaccharide exposure on primary bovine ruminal epithelial cells","name":"articletitle","label":"Article Title"},{"value":"Journal of Dairy Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.3168\/jds.2023-23736","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors.","name":"copyright","label":"Copyright"}]}}