{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:59:26Z","timestamp":1790805566866,"version":"4.1.0"},"reference-count":108,"publisher":"American Dairy Science Association","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001631","name":"University College Dublin","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001631","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202206300032"],"award-info":[{"award-number":["202206300032"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journalofdairyscience.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Dairy Science"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.3168\/jds.2026-28328","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T07:19:45Z","timestamp":1780298385000},"page":"9756-9773","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Graduate Student Literature Review: Standardized approaches for neutrophil functional assessment in dairy cattle\u2014A practical guide"],"prefix":"10.3168","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6537-7632","authenticated-orcid":false,"given":"Lei","family":"Xie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6131-1000","authenticated-orcid":false,"given":"Geert","family":"Opsomer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5305-2133","authenticated-orcid":false,"given":"Osvaldo","family":"Bogado Pascottini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1747","reference":[{"key":"10.3168\/jds.2026-28328_bib1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.17221\/63\/2015-CJAS","article-title":"Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions","volume":"61","author":"Alhussien","year":"2016","journal-title":"Czech J. Anim. Sci."},{"key":"10.3168\/jds.2026-28328_bib2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.3389\/fimmu.2020.00576","article-title":"Circadian features of neutrophil biology","volume":"11","author":"Aroca-Crevill\u00e9n","year":"2020","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib3","doi-asserted-by":"crossref","first-page":"141","DOI":"10.2147\/JIR.S284941","article-title":"Studying neutrophil function in vitro: Cell models and environmental factors","volume":"14","author":"Blanter","year":"2021","journal-title":"J. Inflamm. Res."},{"key":"10.3168\/jds.2026-28328_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetimm.2020.110182","article-title":"Association of metabolic markers with neutrophil function in healthy postpartum dairy cows","volume":"232","author":"Bogado Pascottini","year":"2021","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.3168\/jds.2026-28328_bib5","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.3390\/ani12182403","article-title":"Relationship between oxidative stress and endometritis: Exploiting knowledge gained in mares and cows","volume":"12","author":"Boni","year":"2022","journal-title":"Animals (Basel)"},{"key":"10.3168\/jds.2026-28328_bib6","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1038\/ni.2987","article-title":"Neutrophils sense microbe size and selectively release neutrophil extracellular traps in response to large pathogens","volume":"15","author":"Branzk","year":"2014","journal-title":"Nat. Immunol."},{"key":"10.3168\/jds.2026-28328_bib7","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil extracellular traps kill bacteria","volume":"303","author":"Brinkmann","year":"2004","journal-title":"Science"},{"key":"10.3168\/jds.2026-28328_bib8","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.vetimm.2007.04.002","article-title":"Transportation stress in young bulls alters expression of neutrophil genes important for the regulation of apoptosis, tissue remodeling, margination, and anti-bacterial function","volume":"118","author":"Buckham Sporer","year":"2007","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"Suppl 1","key":"10.3168\/jds.2026-28328_bib9","doi-asserted-by":"crossref","first-page":"E39","DOI":"10.3168\/jds.2006-696","article-title":"Cumulative physiological events influence the inflammatory response of the bovine udder to Escherichia coli infections during the transition period","volume":"90","author":"Burvenich","year":"2007","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2026-28328_bib10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0009-8981(02)00015-3","article-title":"Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA f low cytometry and cytochrome c reduction","volume":"319","author":"Caldefie-Ch\u00e9zet","year":"2002","journal-title":"Clin. Chim. Acta"},{"key":"10.3168\/jds.2026-28328_bib11","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/nrm3624","article-title":"Talins and kindlins: Partners in integrin-mediated adhesion","volume":"14","author":"Calderwood","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.3168\/jds.2026-28328_bib12","doi-asserted-by":"crossref","first-page":"421","DOI":"10.2460\/ajvr.1975.36.04.421","article-title":"Intravascular granulocyte kinetics in developing calves","volume":"36","author":"Carlson","year":"1975","journal-title":"Am. J. Vet. Res."},{"key":"10.3168\/jds.2026-28328_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0311742","article-title":"Effect of type of anticoagulant, transportation time, and glucose in the culture media on neutrophil viability and function test results in dairy cattle","volume":"19","author":"Chandrappa","year":"2024","journal-title":"PLoS One"},{"key":"10.3168\/jds.2026-28328_bib14","article-title":"3D-printed plugs enhance cell usage efficiency for single-cell migration and neuron axon guidance assays","volume":"5","author":"Cheng","year":"2025","journal-title":"Cell Rep. Methods"},{"key":"10.3168\/jds.2026-28328_bib15","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/751014","article-title":"Heparin interaction with the primed polymorphonuclear leukocyte cd11b induces apoptosis and prevents cell activation","volume":"2015","author":"Cohen-Mazor","year":"2015","journal-title":"J. Immunol. Res."},{"key":"10.3168\/jds.2026-28328_bib16","doi-asserted-by":"crossref","first-page":"3667","DOI":"10.1038\/s41598-022-07455-2","article-title":"Optimization of methods for the accurate characterization of whole blood neutrophils","volume":"12","author":"Connelly","year":"2022","journal-title":"Sci. Rep."},{"key":"10.3168\/jds.2026-28328_bib17","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.3390\/nu10111564","article-title":"Glutamine: Metabolism and immune function, supplementation and clinical translation","volume":"10","author":"Cruzat","year":"2018","journal-title":"Nutrients"},{"key":"10.3168\/jds.2026-28328_bib18","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.3390\/cells11182889","article-title":"Neutrophil Activated by the famous and potent PMA (phorbol myristate acetate)","volume":"11","author":"Damascena","year":"2022","journal-title":"Cells"},{"key":"10.3168\/jds.2026-28328_bib19","doi-asserted-by":"crossref","first-page":"6732","DOI":"10.4049\/jimmunol.163.12.6732","article-title":"NADPH oxidase activation and assembly during phagocytosis","volume":"163","author":"DeLeo","year":"1999","journal-title":"J. Immunol."},{"key":"10.3168\/jds.2026-28328_bib20","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/j.psyneuen.2012.05.008","article-title":"Stress-induced redistribution of immune cells-From barracks to boulevards to battlefields: A tale of three hormones\u2014Curt Richter Award Winner","volume":"37","author":"Dhabhar","year":"2012","journal-title":"Psychoneuroendocrinology"},{"key":"10.3168\/jds.2026-28328_bib21","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1089\/ars.2012.4886","article-title":"Methods for detection of mitochondrial and cellular reactive oxygen species","volume":"20","author":"Dikalov","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"10.3168\/jds.2026-28328_bib22","doi-asserted-by":"crossref","first-page":"8720","DOI":"10.1038\/ncomms9720","article-title":"Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions","volume":"6","author":"Doyle","year":"2015","journal-title":"Nat. Commun."},{"key":"10.3168\/jds.2026-28328_bib23","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.3168\/jds.S0022-0302(99)75474-3","article-title":"Biology of dairy cows during the transition period: The final frontier?","volume":"82","author":"Drackley","year":"1999","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2026-28328_bib24","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1083\/jcb.116.1.219","article-title":"Divalent cation regulation of the function of the leukocyte integrin LFA-1","volume":"116","author":"Dransfield","year":"1992","journal-title":"J. Cell Biol."},{"key":"10.3168\/jds.2026-28328_bib25","doi-asserted-by":"crossref","first-page":"9401","DOI":"10.1038\/s41598-025-93929-y","article-title":"Metabolic phenotype of bovine blood-derived neutrophils is altered in milk","volume":"15","author":"Duda","year":"2025","journal-title":"Sci. Rep."},{"key":"10.3168\/jds.2026-28328_bib26","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1002\/cyto.a.20726","article-title":"Flow cytometric investigation of neutrophil oxidative burst and apoptosis in physiological and pathological situations","volume":"75A","author":"Elbim","year":"2009","journal-title":"Cytometry A"},{"key":"10.3168\/jds.2026-28328_bib27","doi-asserted-by":"crossref","first-page":"533","DOI":"10.3390\/mi10080533","article-title":"Permeability of epithelial\/endothelial barriers in Transwells and microfluidic bilayer devices","volume":"10","author":"Frost","year":"2019","journal-title":"Micromachines (Basel)"},{"key":"10.3168\/jds.2026-28328_bib28","doi-asserted-by":"crossref","first-page":"2802","DOI":"10.1128\/IAI.00051-08","article-title":"\u03b2-Hydroxybutyrate abrogates formation of bovine neutrophil extracellular traps and bactericidal activity against mammary pathogenic Escherichia coli","volume":"76","author":"Grinberg","year":"2008","journal-title":"Infect. Immun."},{"key":"10.3168\/jds.2026-28328_bib29","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.809590","article-title":"\u03b22-Integrins\u2014Regulatory and executive bridges in the signaling network controlling leukocyte trafficking and migration","volume":"13","author":"Guenther","year":"2022","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib30","first-page":"85","article-title":"A bovine mammary endothelial\/epithelial cell culture model of the blood\/milk barrier","volume":"188","author":"Guidry","year":"1998","journal-title":"Can. J. Vet. Res."},{"key":"10.3168\/jds.2026-28328_bib31","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetimm.2006.03.022","article-title":"Neutrophil function and energy status in Holstein cows with uterine health disorders","volume":"113","author":"Hammon","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.3168\/jds.2026-28328_bib32","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/JLB.3RU0719-241R","article-title":"Calcium signaling and regulation of neutrophil functions: Still a long way to go","volume":"107","author":"Hann","year":"2020","journal-title":"J. Leukoc. Biol."},{"key":"10.3168\/jds.2026-28328_bib33","doi-asserted-by":"crossref","DOI":"10.1093\/jleuko\/qiae243","article-title":"Glutamine modulates neutrophil recruitment and effector functions during sterile inflammation","volume":"117","author":"Hellenthal","year":"2025","journal-title":"J. Leukoc. Biol."},{"key":"10.3168\/jds.2026-28328_bib34","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0301-472X(99)00150-2","article-title":"Physiologically significant effects of pH and oxygen tension on granulopoiesis","volume":"28","author":"Hevehan","year":"2000","journal-title":"Exp. Hematol."},{"key":"10.3168\/jds.2026-28328_bib35","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/1756-0500-5-93","article-title":"The influence of different anticoagulants and sample preparation methods on measurement of mCD14 on bovine monocytes and polymorphonuclear neutrophil leukocytes","volume":"5","author":"Ibeagha-Awemu","year":"2012","journal-title":"BMC Res. Notes"},{"key":"10.3168\/jds.2026-28328_bib36","doi-asserted-by":"crossref","first-page":"3143","DOI":"10.3389\/fimmu.2018.03143","article-title":"Control of leukocyte trafficking by stress-associated hormones","volume":"9","author":"Ince","year":"2019","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib37","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1017\/S175173111200170X","article-title":"Nutrition, immune function and health of dairy cattle","volume":"7","author":"Ingvartsen","year":"2013","journal-title":"Animal"},{"key":"10.3168\/jds.2026-28328_bib38","doi-asserted-by":"crossref","first-page":"764","DOI":"10.3389\/fcell.2020.00764","article-title":"An efficient nitroblue tetrazolium staining and bright-field microscopy based method for detecting and quantifying intracellular reactive oxygen species in oocytes, cumulus cells and embryos","volume":"8","author":"Javvaji","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"10.3168\/jds.2026-28328_bib39","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/978-1-0716-3052-5_22","article-title":"Transwell in vitro cell migration and invasion assays","volume":"2644","author":"Justus","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"10.3168\/jds.2026-28328_bib40","doi-asserted-by":"crossref","first-page":"207","DOI":"10.2460\/ajvr.1989.50.02.207","article-title":"Alterations in bovine neutrophil function during the periparturient period","volume":"50","author":"Kehrli","year":"1989","journal-title":"Am. J. Vet. Res."},{"key":"10.3168\/jds.2026-28328_bib41","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1002\/jcb.24391","article-title":"Reactive oxygen species-induced activation of ERK and p38 MAPK mediates PMA-induced NETs release from human neutrophils","volume":"114","author":"Keshari","year":"2013","journal-title":"J. Cell. Biochem."},{"key":"10.3168\/jds.2026-28328_bib42","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1290044","article-title":"Enhancing bovine immune, antioxidant and anti-inflammatory responses with vitamins, rumen-protected amino acids, and trace minerals to prevent periparturient mastitis","volume":"14","author":"Khan","year":"2024","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib43","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1177\/2211068214562247","article-title":"Microplatforms for gradient field generation of various properties and biological applications","volume":"20","author":"Kim","year":"2015","journal-title":"J. Lab. Autom."},{"key":"10.3168\/jds.2026-28328_bib44","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/wsbm.32","article-title":"Role of neutrophils in innate immunity: A systems biology-level approach","volume":"1","author":"Kobayashi","year":"2009","journal-title":"Wiley Interdiscip. Rev. Syst. Biol. Med."},{"key":"10.3168\/jds.2026-28328_bib45","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/nri3399","article-title":"Neutrophil recruitment and function in health and inflammation","volume":"13","author":"Kolaczkowska","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"10.3168\/jds.2026-28328_bib46","doi-asserted-by":"crossref","first-page":"8674","DOI":"10.3390\/ijms21228674","article-title":"Glucose concentration in cell culture medium influences the brca1-mediated regulation of the lipogenic action of IGF-I in breast cancer cells","volume":"21","author":"Koobotse","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.3168\/jds.2026-28328_bib47","doi-asserted-by":"crossref","first-page":"14646","DOI":"10.1038\/s41598-020-71500-1","article-title":"Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils","volume":"10","author":"Krabbe","year":"2020","journal-title":"Sci. Rep."},{"key":"10.3168\/jds.2026-28328_bib48","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1301183","article-title":"Isolation methods determine human neutrophil responses after stimulation","volume":"14","author":"Kr\u00e9mer","year":"2023","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib49","doi-asserted-by":"crossref","first-page":"4980","DOI":"10.1021\/acs.jafc.7b01602","article-title":"Differences in the oxylipid profiles of bovine milk and plasma at different stages of lactation","volume":"65","author":"Kuhn","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"10.3168\/jds.2026-28328_bib50","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nri1312","article-title":"NOX enzymes and the biology of reactive oxygen","volume":"4","author":"Lambeth","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"10.3168\/jds.2026-28328_bib51","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.it.2020.03.008","article-title":"How neutrophils meet their end","volume":"41","author":"Lawrence","year":"2020","journal-title":"Trends Immunol."},{"key":"10.3168\/jds.2026-28328_bib52","doi-asserted-by":"crossref","first-page":"s44","DOI":"10.1017\/S1751731119003227","article-title":"Review: Relationships between metabolism and neutrophil function in dairy cows in the peripartum period","volume":"14","author":"LeBlanc","year":"2020","journal-title":"Animal"},{"key":"10.3168\/jds.2026-28328_bib53","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1002\/pro.5560060310","article-title":"Il-8 single-chain homodimers and heterodimers: Interactions with the chemokine receptors CXCR1, CXCR2, and DARC","volume":"6","author":"Leong","year":"1997","journal-title":"Protein Sci."},{"key":"10.3168\/jds.2026-28328_bib54","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1007\/s00204-015-1520-y","article-title":"Interactions between mitochondrial reactive oxygen species and cellular glucose metabolism","volume":"89","author":"Liemburg-Apers","year":"2015","journal-title":"Arch. Toxicol."},{"key":"10.3168\/jds.2026-28328_bib55","doi-asserted-by":"crossref","first-page":"3436","DOI":"10.3168\/jds.2022-22471","article-title":"Viability and function dynamics of circulating versus endometrial polymorphonuclear leukocytes in postpartum dairy cows with subclinical or clinical endometritis","volume":"106","author":"Lietaer","year":"2023","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2026-28328_bib56","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.3390\/ani11041081","article-title":"Flow cytometric assessment of the viability and functionality of uterine polymorphonuclear leukocytes in postpartum dairy cows","volume":"11","author":"Lietaer","year":"2021","journal-title":"Animals (Basel)"},{"key":"10.3168\/jds.2026-28328_bib57","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.theriogenology.2021.11.002","article-title":"Quantitative and functional dynamics of circulating and endometrial polymorphonuclear leukocytes in healthy peripartum dairy cows","volume":"178","author":"Lietaer","year":"2022","journal-title":"Theriogenology"},{"key":"10.3168\/jds.2026-28328_bib58","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.vetimm.2006.05.004","article-title":"Neutrophil extracellular trap formation by bovine neutrophils is not inhibited by milk","volume":"113","author":"Lippolis","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.3168\/jds.2026-28328_bib59","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3389\/fimmu.2018.00288","article-title":"The neutrophil's choice: Phagocytose vs make neutrophil extracellular traps","volume":"9","author":"Manfredi","year":"2018","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib60","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1002\/cyto.a.23169","article-title":"Measurement of NET formation in vitro and in vivo by flow cytometry","volume":"91","author":"Masuda","year":"2017","journal-title":"Cytometry A."},{"key":"10.3168\/jds.2026-28328_bib61","doi-asserted-by":"crossref","first-page":"11025","DOI":"10.1074\/jbc.270.19.11025","article-title":"Leukocyte trafficking mediated by selectin-carbohydrate interactions","volume":"270","author":"McEver","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.3168\/jds.2026-28328_bib62","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1038\/s41423-020-0412-0","article-title":"Neutrophil chemoattractant receptors in health and disease: Double-edged swords","volume":"17","author":"Metzemaekers","year":"2020","journal-title":"Cell. Mol. Immunol."},{"key":"10.3168\/jds.2026-28328_bib63","doi-asserted-by":"crossref","first-page":"2304","DOI":"10.1038\/s41564-023-01513-9","article-title":"A high-throughput and low-waste viability assay for microbes","volume":"8","author":"Meyer","year":"2023","journal-title":"Nat. Microbiol."},{"key":"10.3168\/jds.2026-28328_bib64","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0015309","article-title":"An improved chamber for direct visualisation of chemotaxis","volume":"5","author":"Muinonen-Martin","year":"2010","journal-title":"PLoS One"},{"key":"10.3168\/jds.2026-28328_bib65","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1146\/annurev-pathol-011110-130224","article-title":"Mechanisms of leukocyte transendothelial migration","volume":"6","author":"Muller","year":"2011","journal-title":"Annu. Rev. Pathol."},{"key":"10.3168\/jds.2026-28328_bib66","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1084\/jem.178.2.449","article-title":"PECAM-1 is required for transendothelial migration of leukocytes","volume":"178","author":"Muller","year":"1993","journal-title":"J. Exp. Med."},{"key":"10.3168\/jds.2026-28328_bib67","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/imr.13173","article-title":"The formation and function of the neutrophil phagosome","volume":"314","author":"Naish","year":"2023","journal-title":"Immunol. Rev."},{"key":"10.3168\/jds.2026-28328_bib68","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3389\/fcimb.2017.00373","article-title":"Neutrophils to the ROScue: Mechanisms of NADPH oxidase activation and bacterial resistance","volume":"7","author":"Nguyen","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.3168\/jds.2026-28328_bib69","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.redox.2015.07.009","article-title":"Genetic disorders coupled to ROS deficiency","volume":"6","author":"O'Neill","year":"2015","journal-title":"Redox Biol."},{"key":"10.3168\/jds.2026-28328_bib70","first-page":"745","article-title":"Neutrophil isolation protocol","volume":"23","author":"Oh","year":"2008","journal-title":"J. Vis. Exp."},{"key":"10.3168\/jds.2026-28328_bib71","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0026-0495(95)90162-0","article-title":"Influence of stage of lactation on glucose and glutamine metabolism in isolated enterocytes from dairy cattle","volume":"44","author":"Okine","year":"1995","journal-title":"Metabolism"},{"key":"10.3168\/jds.2026-28328_bib72","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1051\/vetres:2003024","article-title":"The bovine neutrophil: Structure and function in blood and milk","volume":"34","author":"Paape","year":"2003","journal-title":"Vet. Res."},{"key":"10.3168\/jds.2026-28328_bib73","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1189\/jlb.1211601","article-title":"Requirements for NADPH oxidase and myeloperoxidase in neutrophil extracellular trap formation differ depending on the stimulus","volume":"92","author":"Parker","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"10.3168\/jds.2026-28328_bib74","doi-asserted-by":"crossref","first-page":"R15","DOI":"10.1530\/REP-22-0452","article-title":"Genesis of clinical and subclinical endometritis in dairy cows","volume":"166","author":"Pascottini","year":"2023","journal-title":"Reproduction"},{"key":"10.3168\/jds.2026-28328_bib75","doi-asserted-by":"crossref","first-page":"9396","DOI":"10.3168\/jds.2019-16687","article-title":"Technical note: Assessment of neutrophil endocytosis and proteolytic degradation and its relationship with phagocytosis and oxidative burst in dairy cows","volume":"102","author":"Pascottini","year":"2019","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2026-28328_bib76","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.631821","article-title":"Neutrophils: Many ways to die","volume":"12","author":"P\u00e9rez-Figueroa","year":"2021","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib77","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.3390\/diagnostics12071715","article-title":"Neutrophil extracellular traps and neutrophil-derived extracellular vesicles: common players in neutrophil effector functions","volume":"12","author":"Pfister","year":"2022","journal-title":"Diagnostics (Basel)"},{"key":"10.3168\/jds.2026-28328_bib78","doi-asserted-by":"crossref","first-page":"832","DOI":"10.3390\/ani14060832","article-title":"The complex interplay of insulin resistance and metabolic inflammation in transition dairy cows","volume":"14","author":"Qiao","year":"2024","journal-title":"Animals (Basel)"},{"key":"10.3168\/jds.2026-28328_bib79","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/978-1-0716-1060-2_16","article-title":"Isolation of bovine neutrophils by fluorescence- and magnetic-activated cell sorting","volume":"2236","author":"Rambault","year":"2021","journal-title":"Methods Mol. Biol."},{"key":"10.3168\/jds.2026-28328_bib80","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12575-018-0072-y","article-title":"DNA area and NETosis analysis (DANA): A high-throughput method to quantify neutrophil extracellular traps in fluorescent microscope images","volume":"20","author":"Rebernick","year":"2018","journal-title":"Biol. Proced. Online"},{"key":"10.3168\/jds.2026-28328_bib81","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0022-1759(82)90280-0","article-title":"Measurement of chemotaxis in Boyden chamber filter assays. Is the checkerboard correction valid?","volume":"49","author":"Rhodes","year":"1982","journal-title":"J. Immunol. Methods"},{"key":"10.3168\/jds.2026-28328_bib82","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.abb.2011.05.010","article-title":"Cellular magnesium homeostasis","volume":"512","author":"Romani","year":"2011","journal-title":"Arch. Biochem. Biophys."},{"key":"10.3168\/jds.2026-28328_bib83","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/j.cmet.2021.03.018","article-title":"Neutrophils fuel effective immune responses through gluconeogenesis and glycogenesis","volume":"33","author":"Sadiku","year":"2021","journal-title":"Cell Metab."},{"key":"10.3168\/jds.2026-28328_bib84","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1007\/s10753-014-0078-9","article-title":"In vitro neutrophil migration requires protein kinase C-Delta (\u03b4-PKC)-mediated myristoylated alanine-rich C-kinase substrate (MARCKS) phosphorylation","volume":"38","author":"Sheats","year":"2015","journal-title":"Inflammation"},{"key":"10.3168\/jds.2026-28328_bib85","doi-asserted-by":"crossref","DOI":"10.3389\/fcvm.2022.974759","article-title":"Neutrophil circadian rhythm is associated with different outcomes of acute kidney injury due to cholesterol crystal embolism","volume":"9","author":"Shi","year":"2022","journal-title":"Front. Cardiovasc. Med."},{"key":"10.3168\/jds.2026-28328_bib86","doi-asserted-by":"crossref","first-page":"6710","DOI":"10.1073\/pnas.110463197","article-title":"Shear stress-induced apoptosis of adherent neutrophils: A mechanism for persistence of cardiovascular device infections","volume":"97","author":"Shive","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.3168\/jds.2026-28328_bib87","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/978-1-62703-845-4_3","article-title":"Neutrophil isolation from nonhuman species","volume":"1124","author":"Siemsen","year":"2014","journal-title":"Methods Mol. Biol."},{"key":"10.3168\/jds.2026-28328_bib88","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3390\/cells11020191","article-title":"An imaging and computational algorithm for efficient identification and quantification of neutrophil extracellular traps","volume":"11","author":"Singhal","year":"2022","journal-title":"Cells"},{"key":"10.3168\/jds.2026-28328_bib89","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0165-2427(99)00019-7","article-title":"Diapedesis across mammary epithelium reduces phagocytic and oxidative burst of bovine neutrophils","volume":"68","author":"Smits","year":"1999","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.3168\/jds.2026-28328_bib90","doi-asserted-by":"crossref","first-page":"2444","DOI":"10.3390\/ani12182444","article-title":"Oxidative stress induces bovine endometrial epithelial cell damage through mitochondria-dependent pathways","volume":"12","author":"Song","year":"2022","journal-title":"Animals (Basel)"},{"key":"10.3168\/jds.2026-28328_bib91","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1189\/jlb.1110637","article-title":"Neutrophil apoptosis is associated with loss of signal regulatory protein alpha (SIRP\u03b1) from the cell surface","volume":"93","author":"Stenberg","year":"2013","journal-title":"J. Leukoc. Biol."},{"key":"10.3168\/jds.2026-28328_bib92","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.it.2013.02.008","article-title":"Pattern recognition receptor function in neutrophils","volume":"34","author":"Thomas","year":"2013","journal-title":"Trends Immunol."},{"key":"10.3168\/jds.2026-28328_bib93","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/978-1-0716-1162-3_9","article-title":"Visualization and quantification of neutrophil extracellular traps","volume":"2255","author":"Tong","year":"2021","journal-title":"Methods Mol. Biol."},{"key":"10.3168\/jds.2026-28328_bib94","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.624025","article-title":"Complement receptors and their role in leukocyte recruitment and phagocytosis","volume":"9","author":"Vandendriessche","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.3168\/jds.2026-28328_bib95","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1002\/1097-0320(20010401)43:4<290::AID-CYTO1061>3.0.CO;2-X","article-title":"Evaluation of red blood cell lysing solutions in the study of neutrophil oxidative burst by the DCFH assay","volume":"43","author":"Vuorte","year":"2001","journal-title":"Cytometry"},{"key":"10.3168\/jds.2026-28328_bib96","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1172\/JCI0214996","article-title":"Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins","volume":"110","author":"Wang","year":"2002","journal-title":"J. Clin. Invest."},{"key":"10.3168\/jds.2026-28328_bib97","article-title":"The role of G protein-coupled receptor in neutrophil dysfunction during sepsis-induced acute respiratory distress syndrome","volume":"14","author":"Wang","year":"2023","journal-title":"Front. Immunol."},{"key":"10.3168\/jds.2026-28328_bib98","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.bbagen.2013.05.004","article-title":"The challenges of using fluorescent probes to detect and quantify specific reactive oxygen species in living cells","volume":"1840","author":"Winterbourn","year":"2014","journal-title":"Biochim. Biophys. Acta, Gen. Subj."},{"key":"10.3168\/jds.2026-28328_bib99","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/B978-0-12-416618-9.00013-3","article-title":"Methods to monitor ROS production by fluorescence microscopy and fluorometry","volume":"542","author":"Wojtala","year":"2014","journal-title":"Methods Enzymol."},{"key":"10.3168\/jds.2026-28328_bib100","doi-asserted-by":"crossref","first-page":"8046","DOI":"10.3390\/ijms22158046","article-title":"A review of the neutrophil extracellular traps (Nets) from cow, sheep and goat models","volume":"22","author":"Worku","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.3168\/jds.2026-28328_bib101","doi-asserted-by":"crossref","first-page":"11353","DOI":"10.3168\/jds.2025-26711","article-title":"Functional and transcriptomic profiling of bovine circulating neutrophils during in vitro lipopolysaccharide challenge across different energetic states","volume":"108","author":"Xie","year":"2025","journal-title":"J. Dairy Sci."},{"key":"10.3168\/jds.2026-28328_bib102","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.rvsc.2021.06.019","article-title":"Neutrophil extracellular traps in cattle health and disease","volume":"139","author":"Xie","year":"2021","journal-title":"Res. Vet. Sci."},{"key":"10.3168\/jds.2026-28328_bib103","doi-asserted-by":"crossref","first-page":"564","DOI":"10.3390\/ani12050564","article-title":"In vitro production of neutrophils extracellular traps is affected by the lactational stage of dairy cows","volume":"12","author":"Xie","year":"2022","journal-title":"Animals (Basel)"},{"key":"10.3168\/jds.2026-28328_bib104","doi-asserted-by":"crossref","first-page":"894","DOI":"10.3855\/jidc.10767","article-title":"Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation","volume":"12","author":"\u017deki\u0107-Sto\u0161i\u0107","year":"2018","journal-title":"J. Infect. Dev. Ctries."},{"key":"10.3168\/jds.2026-28328_bib105","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/omi.12252","article-title":"The roles of NADPH oxidase in modulating neutrophil effector responses","volume":"34","author":"Zeng","year":"2019","journal-title":"Mol. Oral Microbiol."},{"key":"10.3168\/jds.2026-28328_bib106","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1016\/j.freeradbiomed.2012.06.034","article-title":"Photooxidation of Amplex red to resorufin: Implications of exposing the Amplex red assay to light","volume":"53","author":"Zhao","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"10.3168\/jds.2026-28328_bib107","doi-asserted-by":"crossref","first-page":"216","DOI":"10.20455\/ros.2016.841","article-title":"A highly sensitive chemiluminometric assay for real-time detection of biological hydrogen peroxide formation","volume":"1","author":"Zhu","year":"2016","journal-title":"React. Oxyg. Species (Apex)"},{"key":"10.3168\/jds.2026-28328_bib108","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1083\/jcb.75.2.606","article-title":"Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors","volume":"75","author":"Zigmond","year":"1977","journal-title":"J. Cell Biol."}],"container-title":["Journal of Dairy Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030226028948?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022030226028948?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:27:25Z","timestamp":1790803645000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022030226028948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":108,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["S0022030226028948"],"URL":"https:\/\/doi.org\/10.3168\/jds.2026-28328","relation":{},"ISSN":["0022-0302"],"issn-type":[{"value":"0022-0302","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Graduate Student Literature Review: Standardized approaches for neutrophil functional assessment in dairy cattle\u2014A practical guide","name":"articletitle","label":"Article Title"},{"value":"Journal of Dairy Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.3168\/jds.2026-28328","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s).","name":"copyright","label":"Copyright"}]}}