{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T20:35:50Z","timestamp":1791146150873,"version":"4.1.0"},"reference-count":39,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JNS"],"published-print":{"date-parts":[[2009,9]]},"abstract":"\nObject<\/jats:title>\nCarbonic anhydrase (CA) II and IX are enzymes involved in pH homeostasis and have been shown to be upregulated in several types of cancer. In this study, the authors evaluate the expression of CA II and IX in meningiomas and assess their relationship to patient age, tumor type and grade, tumor sex hormone receptor status, tumor cell proliferation, and tumor recurrence.<\/jats:p><\/jats:sec>\n\nMethods<\/jats:title>\nThis study was conducted in consecutive patients who underwent meningioma surgeries at Tampere University Hospital between 1989 and 1999. The expression of CA II and IX was studied immunohistochemically using a tissue microarray technique and specific antibodies.<\/jats:p><\/jats:sec>\n\nResults<\/jats:title>\nImmunohistological staining with CA II and IX was assessed in 443 primary and 67 recurrent tumor specimens. Of these samples, 455 were benign (WHO Grade I), 49 atypical (Grade II), and 6 malignant (Grade III). Endothelial cells in 14.8% of the tumors stained positively for CA II. Tumor cells were positive for CA IX in 11.6% of the cases. Endothelial CA II expression correlated with increasing histological grade (p = 0.002), and tumor proliferation rates were higher in CA II+ versus CA II\u2212 cases (p = 0.002). Androgen receptor\u2013negative tumors were found to be CA II+ significantly more often than androgen receptor\u2013positive tumors (p = 0.001). No associations were found with the CA IX enzyme.<\/jats:p><\/jats:sec>\n\nConclusions<\/jats:title>\nCarbonic anhydrase II positivity in the endothelium was associated with cell proliferation and malignancy grade. These results suggest that CA II expression is associated with malignant progression of meningiomas and could thus be a target molecule for anticancer therapy.<\/jats:p><\/jats:sec>","DOI":"10.3171\/2008.10.17672","type":"journal-article","created":{"date-parts":[[2009,2,13]],"date-time":"2009-02-13T14:58:21Z","timestamp":1234537101000},"page":"472-477","source":"Crossref","is-referenced-by-count":19,"title":["Carbonic anhydrases in meningiomas: association of endothelial carbonic anhydrase II with aggressive tumor features"],"prefix":"10.3171","volume":"111","author":[{"given":"Katariina","family":"Korhonen","sequence":"first","affiliation":[{"name":"Orton Orthopaedic Hospital, Invalid Foundation, Helsinki;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Kaisa","family":"Parkkila","sequence":"additional","affiliation":[{"name":"Department of Neurology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauli","family":"Helen","sequence":"additional","affiliation":[{"name":"Unit of Neurosurgery, Tampere University Hospital, Tampere, Finland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritva","family":"V\u00e4lim\u00e4ki","sequence":"additional","affiliation":[{"name":"Department of Pathology, Centre for Laboratory Medicine, Tampere University Hospital, Tampere, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Pastorekova","sequence":"additional","affiliation":[{"name":"Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaromir","family":"Pastorek","sequence":"additional","affiliation":[{"name":"Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seppo","family":"Parkkila","sequence":"additional","affiliation":[{"name":"Institute of Medical Technology and School of Medicine, University of Tampere and Tampere University Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannu","family":"Haapasalo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Centre for Laboratory Medicine, Tampere University Hospital, Tampere, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1754","reference":[{"key":"b1-1110472","series-title":"Cancer Res","first-page":"5262","article-title":"Carbonic anhydrase IX, an endogenous hypoxia marker, expression in head and neck squamous cell carcinoma and its relationship to hypoxia, necrosis, and microvessel 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