{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T11:03:41Z","timestamp":1774868621884,"version":"3.50.1"},"reference-count":52,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JNS"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:sec>\n<jats:title>Object<\/jats:title>\n<jats:p>Human amniotic fluid\u2013derived mesenchymal stem cells (AFMSCs) have been shown to promote peripheral nerve regeneration, and the local delivery of neurotrophic factors may additionally enhance nerve regeneration capacity. The present study evaluates whether the transplantation of glia cell line\u2013derived neurotrophic factor (GDNF)\u2013modified human AFMSCs may enhance regeneration of sciatic nerve after a crush injury.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Peripheral nerve injury was produced in Sprague-Dawley rats by crushing the left sciatic nerve using a vessel clamp. Either GDNF-modified human AFMSCs or human AFMSCs were embedded in Matrigel and delivered to the injured nerve. Motor function and electrophysiological studies were conducted after 1 and 4 weeks. Early or later nerve regeneration markers were used to evaluate nerve regeneration. The expression of GDNF in the transplanted human AFMSCs and GDNF-modified human AFMSCs was monitored at 7-day intervals.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Human AFMSCs were successfully transfected with adenovirus, and a significant amount of GDNF was detected in human AFMSCs or the culture medium supernatant. Increases in the sciatic nerve function index, the compound muscle action potential ratio, conduction latency, and muscle weight were found in the groups treated with human AFMSCs or GDNF-modified human AFMSCs. Importantly, the GDNF-modified human AFMSCs induced the greatest improvement. Expression of markers of early nerve regeneration, such as increased expression of neurofilament and BrdU and reduced Schwann cell apoptosis, as well as late regeneration markers, consisting of reduced vacuole counts, increased expression of Luxol fast blue and S100 protein, paralleled the results of motor function. The expression of GDNF in GDNF-modified human AFMSCs was demonstrated up to 4 weeks; however, the expression decreased over time.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The GDNF-modified human AFMSCs appeared to promote nerve regeneration. The consecutive expression of GDNF was demonstrated in GDNF-modified human AFMSCs up to 4 weeks. These findings support a nerve regeneration scenario involving cell transplantation with additional neurotrophic factor secretion.<\/jats:p><\/jats:sec>","DOI":"10.3171\/2009.8.jns09850","type":"journal-article","created":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T14:37:49Z","timestamp":1255099069000},"page":"868-879","source":"Crossref","is-referenced-by-count":49,"title":["Enhancement of regeneration with glia cell line\u2013derived neurotrophic factor\u2013transduced human amniotic fluid mesenchymal stem cells after sciatic nerve crush injury [RETRACTED]"],"prefix":"10.3171","volume":"112","author":[{"given":"Fu-Chou","family":"Cheng","sequence":"first","affiliation":[{"name":"Stem Cell Center, Department of Medical Research, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Hong","family":"Tai","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meei-Ling","family":"Sheu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Department of Medical Research, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dar-Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Lin","family":"Su","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Peng","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, National Chung-Hsing University, 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