{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T13:22:23Z","timestamp":1768310543881,"version":"3.49.0"},"reference-count":21,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JNS"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:sec>\n<jats:title>Object<\/jats:title>\n<jats:p>In the intraluminal suture model of middle cerebral artery occlusion (MCAO) in the mouse, disturbance of blood flow from the internal carotid artery to the posterior cerebral artery (PCA) may affect the size of the infarction. In this study, PCA involvement in the model was investigated and modified for consistent MCAO without involving the PCA territory.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Thirty-seven C57Bl\/6 mice were randomly divided into 4 groups according to the length of coating over the tip of the suture (1, 2, 3, or 4 mm) and subjected to transient MCAO for 2 hours. Real-time topographical cerebral blood flow was monitored over both hemispheres by laser speckle flowmetry. After 24 hours of reperfusion, the infarct territories and volumes were evaluated.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The 1- and 2-mm coating groups showed all lesions in the MCA territory. In the 3- and 4-mm coating groups, 62.5% and 75% of mice, respectively, showed lesions in both the MCA and the PCA territories and other lesions in the MCA territory. Mice in the 1- and 2-mm coating groups had significantly smaller infarct volumes than the 3- and 4-mm groups. Laser speckle flowmetry was useful to distinguish whether the PCA territory would undergo infarction.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Small changes in the coating length of the intraluminal suture may be critical, and 1\u20132 mm of coating appeared to be optimal to produce consistent MCAO without involving the PCA territory. Laser speckle flowmetry could predict the territory of infarction and improve the consistency of the infarct size.<\/jats:p><\/jats:sec>","DOI":"10.3171\/2011.11.jns111167","type":"journal-article","created":{"date-parts":[[2011,12,23]],"date-time":"2011-12-23T15:11:40Z","timestamp":1324653100000},"page":"657-664","source":"Crossref","is-referenced-by-count":21,"title":["Consistent focal cerebral ischemia without posterior cerebral artery occlusion and its real-time monitoring in an intraluminal suture model in mice"],"prefix":"10.3171","volume":"116","author":[{"given":"Yosuke","family":"Akamatsu","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Tohoku University Graduate School of Medicine;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Kohnan Hospital; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Kohnan Hospital; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, National Hospital Organization Sendai Medical Center, Sendai, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teiji","family":"Tominaga","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Tohoku University Graduate School of Medicine;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1754","reference":[{"key":"b1-jns111167","series-title":"J Cereb Blood Flow Metab","first-page":"744","article-title":"Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse 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Metab","first-page":"1274","article-title":"Electron paramagnetic resonance-guided normobaric hyperoxia treatment protects the brain by maintaining penumbral oxygenation in a rat model of transient focal cerebral ischemia","volume":"26","author":"Liu","year":"2006"},{"key":"b14-jns111167","series-title":"Stroke","first-page":"84","article-title":"Reversible middle cerebral artery occlusion without craniectomy in rats","volume":"20","author":"Longa","year":"1989"},{"key":"b15-jns111167","series-title":"Neuroreport","first-page":"1317","article-title":"Differences in the cerebrovascular anatomy of C57black\/6 and SV129 mice","volume":"9","author":"Maeda","year":"1998"},{"key":"b16-jns111167","series-title":"Stroke","first-page":"2707","article-title":"Differences in vulnerability to permanent focal cerebral ischemia among 3 common mouse strains","volume":"31","author":"Majid","year":"2000"},{"key":"b17-jns111167","series-title":"Brain Res","first-page":"304","article-title":"The development of a new mouse model of global ischemia: focus on the relationships between ischemia duration, anesthesia, cerebral vasculature, and neuronal injury following global ischemia in mice","volume":"780","author":"Murakami","year":"1998"},{"key":"b18-jns111167","series-title":"Stroke","first-page":"2162","article-title":"A critical reevaluation of the intraluminal thread model of focal cerebral ischemia: evidence of inadvertent premature reperfusion and subarachnoid hemorrhage in rats by laser-Doppler flowmetry","volume":"29","author":"Schmid-Elsaesser","year":"1998"},{"key":"b19-jns111167","series-title":"Brain Res","first-page":"131","article-title":"Effect of suture size and carotid clip application upon blood flow and infarct volume after permanent and temporary middle cerebral artery occlusion in mice","volume":"970","author":"Tsuchiya","year":"2003"},{"issue":"1 Suppl","key":"b20-jns111167","series-title":"Neurosurgery","first-page":"196","article-title":"Ideal suture diameter is critical for consistent middle cerebral artery occlusion in mice","volume":"56","author":"T\u00fcreyen","year":"2005"},{"key":"b21-jns111167","series-title":"Brain Res","first-page":"209","article-title":"C57BL\/6 strain is most susceptible to cerebral ischemia following bilateral common carotid occlusion among seven mouse strains: selective neuronal death in the murine transient forebrain ischemia","volume":"752","author":"Yang","year":"1997"}],"container-title":["Journal of 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