{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T07:56:00Z","timestamp":1791014160253,"version":"4.1.0"},"reference-count":54,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JNS"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:sec>\n<jats:title>Object<\/jats:title>\n<jats:p>Increased integration of CD34<jats:sup>+<\/jats:sup> cells in injured nerve significantly promotes nerve regeneration, but this effect can be counteracted by limited migration and short survival of CD34<jats:sup>+<\/jats:sup> cells. SDF-1\u03b1 and its receptor mediate the recruitment of CD34<jats:sup>+<\/jats:sup> cells involved in the repair mechanism of several neurological diseases. In this study, the authors investigate the potentiation of CD34<jats:sup>+<\/jats:sup> cell recruitment triggered by SDF-1\u03b1 and the involvement of CD34<jats:sup>+<\/jats:sup> cells in peripheral nerve regeneration.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Peripheral nerve injury was induced in 147 Sprague-Dawley rats by crushing the left sciatic nerve with a vessel clamp. The animals were allocated to 3 groups: Group 1, crush injury (controls); Group 2, crush injury and local application of SDF-1\u03b1 recombinant proteins; and Group 3, crush injury and local application of SDF-1\u03b1 antibody. Electrophysiological studies and assessment of regeneration markers were conducted at 4 weeks after injury; neurobehavioral studies were conducted at 1, 2, 3, and 4 weeks after injury. The expression of SDF-1\u03b1, accumulation of CD34<jats:sup>+<\/jats:sup> cells, immune cells, and angiogenesis factors in injured nerves were evaluated at 1, 3, 7, 10, 14, 21, and 28 days after injury.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Application of SDF-1\u03b1 increased the migration of CD34<jats:sup>+<\/jats:sup> cells in vitro, and this effect was dose dependent. Crush injury induced the expression of SDF-1\u03b1, with a peak of 10\u201314 days postinjury, and this increased expression of SDF-1\u03b1 paralleled the deposition of CD34<jats:sup>+<\/jats:sup> cells, expression of VEGF, and expression of neurofilament. These effects were further enhanced by the administration of SDF-1\u03b1 recombinant protein and abolished by administration of SDF-1\u03b1 antibody. Furthermore, these effects were consistent with improvement in measures of neurological function such as sciatic function index, electrophysiological parameters, muscle weight, and myelination of regenerative nerve.<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Expression of SDF-1\u03b1 facilitates recruitment of CD34<jats:sup>+<\/jats:sup> cells in peripheral nerve injury. The increased deposition of CD34<jats:sup>+<\/jats:sup> cells paralleled significant expression of angiogenesis factors and was consistent with improvement of neurological function. Utilization of SDF-1\u03b1 for enhancing the recruitment of CD34<jats:sup>+<\/jats:sup> cells involved in peripheral nerve regeneration may be considered as an alternative treatment strategy in peripheral nerve disorders.<\/jats:p><\/jats:sec>","DOI":"10.3171\/2011.3.jns101582","type":"journal-article","created":{"date-parts":[[2011,8,19]],"date-time":"2011-08-19T13:59:20Z","timestamp":1313762360000},"page":"432-444","source":"Crossref","is-referenced-by-count":19,"title":["Recruitment by SDF-1\u03b1 of CD34-positive cells involved in sciatic nerve regeneration"],"prefix":"10.3171","volume":"116","author":[{"given":"Meei-Ling","family":"Sheu","sequence":"first","affiliation":[{"name":"Institutes of Biomedical Sciences and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu-Chou","family":"Cheng","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Taichung Veterans General Hospital, Taichung;"},{"name":"Education and Medical Research, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Lin","family":"Su","sequence":"additional","affiliation":[{"name":"Life Sciences, National Chung-Hsing University;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Ju","family":"Chen","sequence":"additional","affiliation":[{"name":"Stem Cell Center, Taichung Veterans General Hospital, Taichung;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Education and Medical Research, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Ming","family":"Chiang","sequence":"additional","affiliation":[{"name":"Radiology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ta","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Taipei Medical University\u2013Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Sheehan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, University of Virginia Health System, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-Chuan","family":"Pan","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and"},{"name":"Departments of Neurosurgery,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1754","reference":[{"key":"b1-jns101582","series-title":"Blood","first-page":"1249","article-title":"Mobilization of hematopoietic stem cells during homeostasis and after cytokine exposure","volume":"102","author":"Abkowitz","year":"2003"},{"key":"b2-jns101582","series-title":"Plast Reconstr Surg","first-page":"129","article-title":"Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat","volume":"83","author":"Bain","year":"1989"},{"key":"b3-jns101582","series-title":"Exp Neurol","first-page":"443","article-title":"Transplantation of bone marrow stromal cells for peripheral nerve repair","volume":"204","author":"Chen","year":"2007"},{"key":"b4-jns101582","series-title":"Brain Res","first-page":"272","article-title":"Glial cell line-derived neurotrophic factor enhances axonal regeneration following sciatic nerve transection in adult rats","volume":"902","author":"Chen","year":"2001"},{"key":"b5-jns101582","series-title":"J Neurosurg","first-page":"868","article-title":"Enhancement of regeneration with glia cell line\u2013derived neurotrophic factor\u2013transduced human amniotic fluid mesenchymal stem cells after sciatic nerve crush injury. 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