{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T01:41:28Z","timestamp":1780105288033,"version":"3.54.0"},"reference-count":28,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JNS"],"published-print":{"date-parts":[[2012,6]]},"abstract":"\nObject<\/jats:title>\nHuman amniotic fluid\u2013derived mesenchymal stem cells (AFMSCs) have been shown to promote peripheral nerve regeneration. The expression of stromal cell\u2013derived factor-1\u03b1 (SDF-1\u03b1) in the injured nerve exerts a trophic effect by recruiting progenitor cells that promote nerve regeneration. In this study, the authors investigated the feasibility of intravenous administration of AFMSCs according to SDF-1\u03b1 expression time profiles to facilitate neural regeneration in a sciatic nerve crush injury model.<\/jats:p><\/jats:sec>\n\nMethods<\/jats:title>\nPeripheral nerve injury was induced in 63 Sprague-Dawley rats by crushing the left sciatic nerve using a vessel clamp. The animals were randomized into 1 of 3 groups: Group I, crush injury as the control; Group II, crush injury and intravenous administration of AFMSCs (5 \u00d7 106<\/jats:sup> cells for 3 days) immediately after injury (early administration); and Group III, crush injury and intravenous administration of AFMSCs (5 \u00d7 106<\/jats:sup> cells for 3 days) 7 days after injury (late administration). Evaluation of neurobehavior, electrophysiological study, and assessment of regeneration markers were conducted every week after injury. The expression of SDF-1\u03b1 and neurotrophic factors and the distribution of AFMSCs in various time profiles were also assessed.<\/jats:p><\/jats:sec>\n\nResults<\/jats:title>\nStromal cell\u2013derived factor-1\u03b1 increased the migration and wound healing of AFMSCs in vitro, and the migration ability was dose dependent. Crush injury induced the expression of SDF-1\u03b1 at a peak of 10\u201314 days either in nerve or muscle, and this increased expression paralleled the expression of its receptor, chemokine receptor type-4 (CXCR-4). Most AFMSCs were distributed to the lung during early or late administration. Significant deposition of AFMSCs in nerve and muscle only occurred in the late administration group. Significantly enhanced neurobehavior, electrophysiological function, nerve myelination, and expression of neurotrophic factors and acetylcholine receptor were demonstrated in the late administration group.<\/jats:p><\/jats:sec>\n\nConclusions<\/jats:title>\nAmniotic fluid\u2013derived mesenchymal stem cells can be recruited by expression of SDF-1\u03b1 in muscle and nerve after nerve crush injury. The increased deposition of AFMSCs paralleled the expression profiles of SDF-1\u03b1 and its receptor CXCR-4 in either muscle or nerve. Administration of AFMSCs led to improvements in neurobehavior and expression of regeneration markers. Intravenous administration of AFMSCs may be a promising alternative treatment strategy in peripheral nerve disorder.<\/jats:p><\/jats:sec>","DOI":"10.3171\/2012.2.jns111360","type":"journal-article","created":{"date-parts":[[2012,4,13]],"date-time":"2012-04-13T13:49:18Z","timestamp":1334324958000},"page":"1357-1367","source":"Crossref","is-referenced-by-count":46,"title":["Dual regeneration of muscle and nerve by intravenous administration of human amniotic fluid\u2013derived mesenchymal stem cells regulated by stromal cell\u2013derived factor-1\u03b1 in a sciatic nerve injury model"],"prefix":"10.3171","volume":"116","author":[{"given":"Dar-Yu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Chang Bing Show Chwan Memorial Hospital, Changhua;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meei-Ling","family":"Sheu","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Lin","family":"Su","sequence":"additional","affiliation":[{"name":"Life Sciences, National Chung-Hsing University, Taichung;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu-Chou","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery,"},{"name":"Department of Education and Medical Research, Taichung Veterans General Hospital, Taichung;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Ju","family":"Chen","sequence":"additional","affiliation":[{"name":"Stem Cell Center, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Education and Medical Research, Taichung Veterans General Hospital, Taichung;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ta","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Taipei Medical University-Shuang Ho Hospital, Taipei, Taiwan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Jean","family":"Yiin","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Sheehan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, University of Virginia, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-Chuan","family":"Pan","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and"},{"name":"Department of Neurosurgery,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1754","reference":[{"key":"b1-jns111360","series-title":"Plast Reconstr Surg","first-page":"129","article-title":"Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat","volume":"83","author":"Bain","year":"1989"},{"key":"b2-jns111360","series-title":"J Neurosurg","first-page":"868","article-title":"Enhancement of regeneration with glia cell line-derived neurotrophic factor-transduced human amniotic fluid mesenchymal stem cells after sciatic nerve crush injury. 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