{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T21:06:31Z","timestamp":1784063191039,"version":"3.55.0"},"reference-count":26,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1984,8]]},"abstract":"<jats:p content-type=\"fine-print\">\u2713 Thirty minutes after experimental spinal cord contusion (500 gm-cm) injury, cats were treated with an initial intravenous dose of either vehicle (V) or 30 mg\/kg of Solu-Medrol sterile powder (methylprednisolone sodium succinate; MPSS). Two hours later, cats received a second intravenous injection of either V or 15 mg\/kg MPSS, giving three treatment groups: V\/V; MPSS\/V; MPSS\/MPSS. At 4\u00bd hours following injury of the cat lumbar spinal cord, the gray and white matter neurofilament protein content was reduced by over 70% within the injured segment of V\/V-treated animals. The three major cat spinal cord neurofilament protein subunits of 200,000, 152,000, and 76,000 daltons were reduced in parallel by the injury. Treatment of cats with a single 30-mg\/kg dose of MPSS (MPSS\/V) provided a clear, although not significant, protection against neurofilament degradation compared with V\/V-treated cats when measured at 4\u00bd hours after injury. The lactic acid content of the injured spinal cord segment at 4\u00bd hours after injury was significantly elevated in both V\/V- and MPSS\/V-treated cats, while the adenosine triphosphate (ATP) content, total adenylates, and energy charge were significantly reduced. The administration of a second intravenous 15-mg\/kg dose of MPSS 2 hours after the initial 30-mg\/kg dose (MPSS\/MPSS) provided complete (p &lt; 0.01) preservation of neurofilaments within the injured spinal cord segment measured at 4\u00bd hours after injury. The levels of lactate, ATP, total adenylates, and tissue energy charge in MPSS\/MPSS-treated cats were not different from those of uninjured spinal cords following laminectomy. The (Na<jats:sup>+<\/jats:sup> + K<jats:sup>+<\/jats:sup>)-ATPase activity in the injured spinal segment was enhanced, although highly variable, in MPSS\/V-treated animals. On the other hand, spinal cord enzyme activity was significantly and consistently elevated in the MPSS\/MPSS-treated group. The results demonstrate that a 30-mg\/kg dose of MPSS followed at 2 hours by a 15-mg\/kg dose provides significantly better protection against injury-induced ischemia and Ca<jats:sup>++<\/jats:sup>-dependent neurofilament degradation than a single 30-mg\/kg dose. These findings are in agreement with the spinal cord tissue pharmacokinetics and time-action characteristics of methylprednisolone observed in earlier studies.<\/jats:p>","DOI":"10.3171\/jns.1984.61.2.0290","type":"journal-article","created":{"date-parts":[[2009,5,8]],"date-time":"2009-05-08T16:21:56Z","timestamp":1241799716000},"page":"290-295","source":"Crossref","is-referenced-by-count":170,"title":["Effects of multi-dose methylprednisolone sodium succinate administration on injured cat spinal cord neurofilament degradation and energy metabolism"],"prefix":"10.3171","volume":"61","author":[{"given":"J. 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