{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T12:42:58Z","timestamp":1760100178509},"reference-count":32,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1986,3]]},"abstract":"<jats:p content-type=\"fine-print\">\u2713 The effect of hemoglobin on endothelium-dependent vasodilation of the isolated rabbit basilar artery was examined using an isometric tension recording method. Acetylcholine (ACh) (10<jats:sup>\u22127<\/jats:sup>\u221210<jats:sup>\u22124<\/jats:sup> M) evoked a dose-dependent vasodilation of isolated rabbit basilar artery previously contracted by 10<jats:sup>\u22126<\/jats:sup> M serotonin. This vasodilating action disappeared after removal of the endothelium. The ACh-induced vasodilation of rabbit basilar artery is thought to be strictly endothelium-dependent. Hemoglobin (10<jats:sup>\u22127<\/jats:sup>-10<jats:sup>\u22125<\/jats:sup> M) inhibited this ACh-induced endothelium-dependent vasodilation conditional upon the dose. Adenosine triphosphate (ATP, 10<jats:sup>\u22127<\/jats:sup>-10<jats:sup>\u22124<\/jats:sup> M) also relaxed isolated rabbit basilar artery already contracted by 10<jats:sup>\u22126<\/jats:sup> M serotonin. This vasodilating action was slightly inhibited by adenosine antagonist, 8-phenyltheophylline (8-PT), and markedly attenuated by removal of the endothelium. This ATP-induced vasodilation is thought to be composed of ATP itself (endothelium-dependent) and ATP degradation products (endothelium-independent) such as adenosine monophosphate or adenosine. Hemoglobin markedly inhibited ATP-induced vasodilation, but there still remained a small vasodilation, which was blocked by 8-PT. Papaverine-induced vasodilation was not affected by removal of the endothelium, and hemoglobin did not inhibit the papaverine-induced vasodilation. These results suggest that rabbit basilar artery has endothelium-dependent vasodilating mechanisms induced by ACh and ATP, and that hemoglobin selectively blocks the endothelium-dependent vasodilation. This finding may relate to the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage: there is a possibility that the presence of hemoglobin released from lysed erythrocytes inhibits the endothelium-dependent vasodilation of cerebral arteries; furthermore, the endothelial degeneration following subarachnoid hemorrhage may impair the vasodilating mechanisms of cerebral artery smooth-muscle cells.<\/jats:p>","DOI":"10.3171\/jns.1986.64.3.0445","type":"journal-article","created":{"date-parts":[[2009,5,8]],"date-time":"2009-05-08T16:15:08Z","timestamp":1241799308000},"page":"445-452","source":"Crossref","is-referenced-by-count":109,"title":["Selective hemoglobin inhibition of endothelium-dependent vasodilation of rabbit basilar artery"],"prefix":"10.3171","volume":"64","author":[{"given":"Shigeru","family":"Fujiwara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neal F.","family":"Kassell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomio","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadayoshi","family":"Nakagomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard M.","family":"Lehman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1754","reference":[{"key":"b1","series-title":"Clin Neurosurg","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1093\/neurosurgery\/24.CN_suppl_1.216","article-title":"Experimental models of spasm.","volume":"24","author":"Alksne","year":"1977"},{"key":"b2","series-title":"Proc Natl Acad Sci USA","doi-asserted-by":"publisher","first-page":"2106","DOI":"10.1073\/pnas.79.6.2106","article-title":"Role of endothelial cells in relaxation of isolated arteries by bradykinin.","volume":"79","author":"Cherry","year":"1982"},{"key":"b3","series-title":"Brain","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1093\/brain\/87.3.491","article-title":"The pathogenesis of cerebral infarction following the rupture of cerebral berry aneurysms.","volume":"87","author":"Crompton","year":"1964"},{"key":"b4","series-title":"J Pharmacol Exp Ther","first-page":"166","article-title":"Endothelium-dependent inhibitory effects of acetylcholine, adenosine triphosphate, thrombin and arachidonic acid in the canine femoral artery.","volume":"222","author":"De Mey","year":"1982"},{"key":"b5","series-title":"J Physiol (Lond)","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1113\/jphysiol.1981.sp013792","article-title":"Role of the intima in cholinergic and purinergic relaxation of isolated canine femoral arteries.","volume":"316","author":"De Mey","year":"1981"},{"key":"b6","series-title":"J Biol Chem","first-page":"51","article-title":"Spectrophotometric studies. 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