{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T01:57:44Z","timestamp":1775267864660,"version":"3.50.1"},"reference-count":31,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:p content-type=\"fine-print\">\u2713 Flow cytometry was performed on stored frozen tissues and explant cell cultures from 39 meningiomas using ethidium bromide and mithramycin in a selective staining technique for deoxyribonucleic acid (DNA). The ploidy index and percentage of cells in the G<jats:sub>0<\/jats:sub>\/G<jats:sub>1<\/jats:sub>, S, and G<jats:sub>2<\/jats:sub>\/M phases were calculated for each specimen. The results were compared with the age and sex of the patients; the site, the histological subtype, and mitotic rate of the neoplasms; and the estrogen and progesterone-receptor levels assayed in cytosol-enriched supernatants from cryostat-cut sections. Sixteen neoplasms (41%) were aneuploid. These included two recurrent neoplasms, seven of the eight neoplasms from patients with multiple meningiomas, and three clinically aggressive neoplasms (one hemangiopericytic and two anaplastic meningiomas). Significant correlations were found between values for the ploidy index (r =0.75, p &lt; 0.01), the percentage of S-phase cells (r = 0.82, p &lt; 0.01), and the percentage of G<jats:sub>2<\/jats:sub>\/M-phase cells (r = 0.69, p &lt; 0.05) <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. The results support the suggestion that flow cytometry for DNA in meningiomas may be of value in predicting the behavior of these neoplasms, and indicate that under controlled conditions explant cell cultures may provide a useful model for the proliferative characteristics of meningiomas <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.3171\/jns.1987.66.4.0588","type":"journal-article","created":{"date-parts":[[2009,5,8]],"date-time":"2009-05-08T16:18:00Z","timestamp":1241799480000},"page":"588-594","source":"Crossref","is-referenced-by-count":51,"title":["DNA in meningioma tissues and explant cell cultures"],"prefix":"10.3171","volume":"66","author":[{"given":"James W.","family":"Ironside","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D. E.","family":"Battersby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Lawry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reginald S.","family":"Loomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher A.","family":"Day","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter R.","family":"Timperley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1754","reference":[{"key":"b1","series-title":"Surg Neurol","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/0090-3019(83)90052-6","article-title":"Flow fluorescence cytometry of deoxyribonucleic acid in meningiomas: studies on surgically removed tumor specimens compared with their cells in primary tissue cultures.","volume":"20","author":"Ahyai","year":"1983"},{"key":"b2","series-title":"Br J Cancer","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1038\/bjc.1979.168","article-title":"Prognostic significance of modal DNA value and other factors in malignant tumors, based on 1465 cases.","volume":"40","author":"Atkin","year":"1979"},{"key":"b3","series-title":"Anal Quant Cytol","first-page":"161","article-title":"DNA content and survival in mammary carcinoma.","volume":"2","author":"Auer","year":"1980"},{"key":"b4","series-title":"Cancer Res","first-page":"4400","article-title":"Correlation of DNA distribution abnormalities with cytogenetic findings in human adult leukemia and lymphoma.","volume":"37","author":"Barlogie","year":"1977"},{"key":"b5","series-title":"J Neurol Neurosurg Psychiatry","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1136\/jnnp.49.4.362","article-title":"Inherited multiple meningiomas: a clinical, pathological and cytogenetic study of an affected family.","volume":"49","author":"Batersby","year":"1986"},{"key":"b6","series-title":"Cancer","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1002\/1097-0142(19821101)50:9<1771::AID-CNCR2820500921>3.0.CO;2-7","article-title":"Estrogen receptor content and ploidy of human mammary carcinoma.","volume":"50","author":"Bichel","year":"1982"},{"key":"b7","series-title":"J Neuropathol Exp Neurol","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1097\/00005072-198505000-00029","article-title":"The relationship between flow cytometric features and clinical behavior of meningiomas.","volume":"44","author":"Crone","year":"1985"},{"key":"b8","series-title":"Hum Pathol","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0046-8177(81)80235-3","article-title":"Mitosis counting \u2014 a need for reappraisal.","volume":"12","author":"Ellis","year":"1981"},{"key":"b9","series-title":"Flow Cytometry IV.","first-page":"398","article-title":"Sequential flow analysis of the single cell DNA content in recurrent human brain tumours","author":"Frederiksen","year":"1980"},{"key":"b10","series-title":"Acta Neuropathol","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/BF00684806","article-title":"DNA content of meningiomas.","volume":"46","author":"Frederiksen","year":"1979"},{"key":"b11","series-title":"J Clin Pathol","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1136\/jcp.37.9.961","article-title":"Clinical and biological significance of aneuploidy in human tumours.","volume":"37","author":"Friedlander","year":"1984"},{"key":"b12","series-title":"Int J Gynecol Pathol","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1097\/00004347-198301000-00005","article-title":"Ploidy as a prognostic factor in ovarian cancer.","volume":"2","author":"Friedlander","year":"1983"},{"key":"b13","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"13","DOI":"10.3171\/jns.1978.49.1.0013","article-title":"The distribution of nuclear DNA from human brain-tumor cells. Flow cytometric studies.","volume":"49","author":"Hoshino","year":"1978"},{"key":"b14","series-title":"J Clin Pathol","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1136\/jcp.39.1.44","article-title":"Cryostat section assay of oestrogen and progesterone receptors in meningiomas: a clinicopathological study.","volume":"39","author":"Ironside","year":"1986"},{"key":"b15","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"757","DOI":"10.3171\/jns.1985.62.5.0757","article-title":"Modulation of meningioma cell growth by sex steroid hormones in vitro.","volume":"62","author":"Jay","year":"1985"},{"key":"b16","series-title":"Acta Neuropathol","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1007\/BF00691065","article-title":"Flow-through cytometry of meningiomas and cultured meningioma cells.","volume":"44","author":"Kajikawa","year":"1978"},{"key":"b17","series-title":"Cytometry","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/cyto.990020102","article-title":"Clinical application of flow cytometry: a review.","volume":"2","author":"Laerum","year":"1981"},{"key":"b18","series-title":"Acta Neuropathol","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1007\/BF00690752","article-title":"Flow-through fluorocytophotometry of different brain tumours.","volume":"49","author":"Lehmann","year":"1980"},{"key":"b19","series-title":"Adv Cancer Res","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/S0065-230X(08)61015-5","article-title":"Chromosomal abnormalities and their specificity in human neoplasms: an assessment of recent observations by banding techniques.","volume":"24","author":"Mark","year":"1977"},{"key":"b20","series-title":"Cancer","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1002\/1097-0142(19810815)48:4<980::AID-CNCR2820480421>3.0.CO;2-7","article-title":"Flow cytometry of breast carcinoma: I. Relation of DNA ploidy level to histology and estrogen receptor.","volume":"48","author":"Olszewski","year":"1981"},{"key":"b21","series-title":"Pathology of Tumours of the Nervous System","first-page":"48","author":"Russell","year":"1977"},{"key":"b22","series-title":"J Neuropathol Exp Neurol","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1097\/00005072-198605000-00012","article-title":"An ultrastructural and immunocytochemical analysis of leptomeningeal and meningioma cultures.","volume":"45","author":"Rutka","year":"1986"},{"key":"b23","series-title":"The Chromosomes in Human Cancer and Leukemia.","author":"Sandberg","year":"1980"},{"key":"b24","series-title":"Br J Cancer","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1038\/bjc.1985.80","article-title":"Tumour ploidy, response and survival in patients receiving endocrine therapy for advanced breast cancer.","volume":"51","author":"Stuart-Harris","year":"1985"},{"key":"b25","series-title":"Br J Cancer","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1038\/bjc.1966.53","article-title":"Tumour ploidy and prognosis in carcinomas of the bladder and prostate.","volume":"20","author":"Tavares","year":"1966"},{"key":"b26","series-title":"J Histochem Cytochem","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.1177\/28.11.6159392","article-title":"An evaluation of DNA fluorochromes, staining techniques, and analysis for flow cytometry. I. Unperturbed cell populations.","volume":"28","author":"Taylor","year":"1980"},{"key":"b27","series-title":"Cytometry","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1002\/cyto.990030502","article-title":"Long-term storage of samples for flow cytometric DNA analysis.","volume":"3","author":"Vindel\u00f8v","year":"1983"},{"key":"b28","series-title":"Cancer Res","first-page":"4295","article-title":"Clonal heterogeneity of small-cell anaplastic carcinoma of the lung demonstrated by flow-cytometric DNA analysis.","volume":"40","author":"Vindel\u00f8v","year":"1980"},{"key":"b29","series-title":"Cancer Genet Cytogenet","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/0165-4608(82)90063-2","article-title":"Cytological and cytogenetical studies on human meningioma.","volume":"6","author":"Zang","year":"1982"},{"key":"b30","series-title":"Scand J Urol Nephrol Suppl","first-page":"53","article-title":"Prognostic significance of nuclear DNA levels in prostatic cancer.","volume":"55","author":"Zetterberg","year":"1980"},{"key":"b31","series-title":"Histological Typing of Tumours of the Central Nervous System.","first-page":"53","author":"Z\u00fclch","year":"1979"}],"container-title":["Journal of Neurosurgery"],"original-title":[],"link":[{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/66\/4\/article-p588.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/66\/4\/article-p588.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/66\/4\/article-p588.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,24]],"date-time":"2022-07-24T16:25:03Z","timestamp":1658679903000},"score":1,"resource":{"primary":{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/66\/4\/article-p588.xml"}},"subtitle":["A flow cytometric study with clinicopathological correlates"],"short-title":[],"issued":{"date-parts":[[1987,4]]},"references-count":31,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.3171\/jns.1987.66.4.0588","relation":{},"ISSN":["0022-3085"],"issn-type":[{"value":"0022-3085","type":"print"}],"subject":[],"published":{"date-parts":[[1987,4]]}}}