{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T12:40:41Z","timestamp":1770986441311,"version":"3.50.1"},"reference-count":34,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,2]]},"abstract":"<jats:p content-type=\"fine-print\">\u2713 A novel antibody-toxin conjugate has been developed for use in cancer therapy. This report demonstrates that this new reagent selectively kills glioblastoma- and medulloblastoma-derived cell lines, medulloblastoma cells in primary culture, and cell lines derived from tumors commonly metastatic to the cerebrospinal fluid (CSF). Efficient killing of human tumor cells occurred at concentrations between 3.9 \u00d7 10<jats:sup>\u221213<\/jats:sup> M and 1.1 \u00d7 10<jats:sup>\u221210<\/jats:sup> M, whereas guinea pigs and rhesus monkeys tolerated intrathecal levels of 2 \u00d7 10<jats:sup>\u22129<\/jats:sup> M. Cerebrospinal fluid from normal humans and from brain-tumor patients does not inhibit the <jats:italic>in vitro<\/jats:italic> efficacy of this reagent. The wide therapeutic window, extreme potency, and general applicability of this antibody-toxin conjugate against CSF-borne primary or metastatic tumors warrants clinical trials.<\/jats:p>","DOI":"10.3171\/jns.1989.70.2.0240","type":"journal-article","created":{"date-parts":[[2009,5,8]],"date-time":"2009-05-08T16:18:41Z","timestamp":1241799521000},"page":"240-248","source":"Crossref","is-referenced-by-count":77,"title":["Improved tumor-specific immunotoxins in the treatment of CNS and leptomeningeal neoplasia"],"prefix":"10.3171","volume":"70","author":[{"given":"Virginia G.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Wrobel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debra","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Zovickian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry","family":"Greenfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward H.","family":"Oldfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Youle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1754","reference":[{"key":"b1","series-title":"Annu Rev Biochem","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1146\/annurev.bi.49.070180.002041","article-title":"Iron transport and storage proteins.","volume":"49","author":"Aisen","year":"1980"},{"key":"b2","series-title":"Cancer Res","first-page":"1214","article-title":"Evaluation of monoclonal antibodies for the development of breast cancer immunotoxins.","volume":"45","author":"Bjorn","year":"1985"},{"key":"b3","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"80","DOI":"10.3171\/jns.1987.66.1.0080","article-title":"A review of the factors influencing the prognosis of medulloblastoma. 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