{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T08:49:10Z","timestamp":1782809350382,"version":"3.54.5"},"reference-count":67,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,5]]},"abstract":"<jats:p content-type=\"fine-print\">\u2713 The cytotoxic activity of immunotoxins constructed with human diferric transferrin (Tfn) as the carrier ligand and an abrin variant <jats:italic>Pseudomonas<\/jats:italic> exotoxin A (PE) and the diphtheria toxin mutant cross-reacting material (CRM) 107 as the toxin moieties were studied <jats:italic>in vitro<\/jats:italic>. Three malignant human cell lines, the glioblastomas multiforme SNB19 and SF295 and the LOX melanoma, and a nonhuman control murine melanoma cell line B16 were assessed. The presence of transferrin receptors on the cell lines was confirmed by direct <jats:sup>125<\/jats:sup>I-Tfn binding assays. The 50% protein synthesis inhibitory concentration (IC<jats:sub>50<\/jats:sub>) values for all cell lines demonstrated that Tfn-abrin variant and Tfn-PE had comparable potency and were both more effective than Tfn-CRM 107. Monensin, a carboxylic ionophore, potentiated the effect of Tfn-abrin variant against glioma cells approximately 35-fold with IC<jats:sub>50<\/jats:sub> values of 4.0 \u00d7 10<jats:sup>\u221213<\/jats:sup> M and 4.7 \u00d7 10<jats:sup>\u221212<\/jats:sup> M for SNB19 and SF295, respectively. Cytotoxic activity of Tfn-abrin variant (with or without monensin) and Tfn-PE was correlated with the degree of Tfn receptor expression measured on the cell lines. The exquisite <jats:italic>in vitro<\/jats:italic> cytotoxicity of Tfn-abrin variant and Tfn-PE immunotoxins against glioma and melanoma cells warrants further <jats:italic>in vivo<\/jats:italic> evaluation and future consideration of these agents for potential clinical application against glioblastoma multiforme and leptomeningeal neoplasia.<\/jats:p>","DOI":"10.3171\/jns.1992.76.5.0838","type":"journal-article","created":{"date-parts":[[2009,5,26]],"date-time":"2009-05-26T20:33:37Z","timestamp":1243370017000},"page":"838-844","source":"Crossref","is-referenced-by-count":37,"title":["In vitro efficacy of transferrin-toxin conjugates against glioblastoma multiforme"],"prefix":"10.3171","volume":"76","author":[{"given":"Walter A.","family":"Hall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aslak","family":"Godal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siri","family":"Juell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d8ystein","family":"Fodstad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1754","reference":[{"key":"b1","series-title":"Cancer Res","first-page":"1005","article-title":"Potentiation of cytotoxic activity of immunotoxins on cultured human cells.","volume":"45","author":"Akiyama","year":"1985"},{"key":"b2","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"175","DOI":"10.3171\/jns.1989.70.2.0175","article-title":"Intratumoral LAK cell and interleukin-2 therapy of human gliomas.","volume":"70","author":"Barba","year":"1989"},{"key":"b3","series-title":"Neurosurgery","first-page":"320","article-title":"Biology of gliomas: potential clinical implications of glioma cellular heterogeneity.","volume":"9","author":"Bigner","year":"1981"},{"key":"b4","series-title":"Nature","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1038\/290145a0","article-title":"Immunotoxins: hybrid molecules of monoclonal antibodies and a toxin subunit specifically kill tumour cells.","volume":"290","author":"Blythman","year":"1981"},{"key":"b5","series-title":"Cancer","doi-asserted-by":"publisher","first-page":"2105","DOI":"10.1002\/1097-0142(19901115)66:10<2105::AID-CNCR2820661011>3.0.CO;2-I","article-title":"Surgical treatment of brain metastases in malignant melanoma.","volume":"66","author":"Brega","year":"1990"},{"key":"b6","series-title":"J Neurosurg","doi-asserted-by":"publisher","first-page":"2","DOI":"10.3171\/jns.1985.63.1.0002","article-title":"Applications of monoclonal antibodies in the diagnosis and treatment of primary brain tumors.","volume":"63","author":"Bullard","year":"1985"},{"key":"b7","series-title":"JNCI","first-page":"673","article-title":"Relationship between antigen density and immunotherapeutic response elicited by monoclonal antibodies against solid tumors.","volume":"72","author":"Capone","year":"1984"},{"key":"b8","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"9359","DOI":"10.1016\/S0021-9258(17)42707-4","article-title":"Kinetics of cytotoxicity induced by immunotoxins. 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