{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T09:29:50Z","timestamp":1749634190732},"reference-count":23,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p content-type=\"fine-print\">\u2713 Forty-nine primary intracranial tumors (33 astrocytomas, eight acoustic schwannomas, five oligodendrogliomas, and three miscellaneous tumors) were studied by flow cytometry. Each tumor specimen was divided into two portions: one was studied as an unfixed suspension stained with propidium iodide and the other after formalin fixation and paraffin embedding. The 50-<jats:italic>\u00b5<\/jats:italic>m sections from the paraffin blocks were deparaffinized, rehydrated, and enzymatically disintegrated, and the cells in suspension were stained with propidium iodide. Flow cytometry of the two portions showed a significant correlation between fresh and fixed specimens regarding the S-phase (r = 0.87) and a very close correlation of the deoxyribonucleic acid (DNA) index (r = 0.95). When the 33 astrocytomas were analyzed separately, similar results were obtained (r = 0.86 for S-phase and r = 0.93 for DNA index, respectively).<\/jats:p>\n<jats:p content-type=\"fine-print\">This study demonstrated a high correlation between fresh and fixed specimens for DNA ploidy and S-phase both in primary intracranial tumors in general and also in the selected subgroup of astrocytomas.<\/jats:p>","DOI":"10.3171\/jns.1994.80.1.0085","type":"journal-article","created":{"date-parts":[[2009,5,28]],"date-time":"2009-05-28T15:21:29Z","timestamp":1243524089000},"page":"85-89","source":"Crossref","is-referenced-by-count":8,"title":["DNA index and S-phase in primary brain 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