{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:54:26Z","timestamp":1790178866978,"version":"4.1.0"},"reference-count":23,"publisher":"Journal of Neurosurgery Publishing Group (JNSPG)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,9]]},"abstract":"\u2713 The platelet-derived growth factor (PDGF) family consists of subunits A and B and receptors \u03b1 and \u03b2. This paper evaluates the potential role of the homodimer PDGF-BB as a growth factor in meningiomas. It analyzes the expression of messenger RNA in members of the PDGF family in these tumors, measures the growth response of meningiomas to exogenous PDGF-BB in culture, and examines the induction of the c-fos<\/jats:italic> proto-oncogene by PDGF-BB. Northern blot analysis was carried out on tissue from 20 meningiomas to measure the expression of PDGF-A, PDGF-B, PDGF-\u03b1 receptor (PDGF-\u03b1-R) and PDGF-\u03b2 receptor (PDGF-\u03b2-R). All tumors expressed PDGF-A and PDGF-B subunits. Nineteen of the 20 tumors expressed PDGF-\u03b2-R and none expressed PDGF-\u03b1-R as measured by this technique.<\/jats:p>\nBecause the \u03b2 receptor is selectively sensitive to stimulation by the PDGF-B subunit, these data suggest that meningiomas might be susceptible to stimulation by PDGF-BB. To test this hypothesis, the effect of exogenous PDGF-BB on meningioma growth was evaluated by incubating cells from 10 human meningiomas. Tritiated thymidine incorporation was used to evaluate stimulation of growth over a 48-hour period using PDGF-BB concentrations of 1, 3, or 6 ng\/ml. Linear regression analysis and multiple-factor analysis of variance were used to measure PDGF-BB effects. Three of the 10 tumor specimens responded significantly to PDGF-BB, with a three- to sixfold increase in thymidine incorporation over 72 hours of exposure, and there was a significant overall growth-stimulating effect of PDGF-BB in the 10 tumor specimens tested. In the last set of experiments, the functionality of the PDGF-\u03b2-R was determined by examining the induction of the proto-oncogene c-fos<\/jats:italic> by PDGF-BB in meningioma cell cultures. A significant increase in c-fos<\/jats:italic> protein was observed 3 hours after PDGF-BB addition.<\/jats:p>\nThese findings demonstrate that PDGF-A, PDGF-B, and PDGF-\u03b2-R are expressed in meningiomas and suggest that the \u03b2 receptor is functional: when it is activated, c-fos<\/jats:italic> levels are increased, and an increase in meningioma cell division is observed after the addition of PDGF-BB. These studies support the hypothesis that PDGF acts as a growth factor in meningiomas.<\/jats:p>","DOI":"10.3171\/jns.1994.81.3.0388","type":"journal-article","created":{"date-parts":[[2009,5,28]],"date-time":"2009-05-28T14:27:01Z","timestamp":1243520821000},"page":"388-393","source":"Crossref","is-referenced-by-count":80,"title":["Platelet-derived growth factor expression and stimulation in human meningiomas"],"prefix":"10.3171","volume":"81","author":[{"given":"Peter McL.","family":"Black","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rona","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danuta","family":"Glowacka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Riley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Dashner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1754","reference":[{"key":"b1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1227\/00006123-199304000-00023","volume":"32","author":"Black PML","year":"1993","journal-title":"Neurosurgery"},{"key":"b2","first-page":"1312","volume":"53","author":"Carroll R","year":"1993","journal-title":"Cancer Res"},{"key":"b3","doi-asserted-by":"publisher","DOI":"10.1021\/bi00591a005"},{"key":"b4","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0092-8674(91)90638-F","volume":"64","author":"Cross M","year":"1991","journal-title":"Cell"},{"key":"b5","first-page":"6462","volume":"48","author":"Elashry-Stowers D","year":"1988","journal-title":"Cancer Res"},{"key":"b6","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1987.66.3.0405"},{"key":"b7","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1991.74.6.0861"},{"key":"b8","first-page":"211","volume-title":"Astrocytomas: Diagnosis, Management, and Biology.","author":"Guha A","year":"1992"},{"key":"b9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF00182081","volume":"8","author":"Harsh GR","year":"1990","journal-title":"J Neurooncol"},{"key":"b10","doi-asserted-by":"crossref","first-page":"7748","DOI":"10.1073\/pnas.85.20.7748","volume":"85","author":"Hermansson M","year":"1988","journal-title":"Proc Natl Acad Sci USA"},{"key":"b11","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1990.72.5.0786"},{"key":"b12","volume-title":"Meningiomas. Biology, Pathology and Differential Diagnosis.","author":"Kepes JJ","year":"1982"},{"key":"b13","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/312711a0","volume":"312","author":"Kruijer W","year":"1985","journal-title":"Nature"},{"key":"b14","doi-asserted-by":"publisher","DOI":"10.1016\/0090-3019(87)90130-3"},{"key":"b15","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1987.67.2.0237"},{"key":"b16","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0090-3019(88)90093-6","volume":"30","author":"Markwalder TM","year":"1988","journal-title":"Surg Neurol"},{"key":"b17","first-page":"159","volume-title":"Advances in Neurooncology.","author":"Maxwell M","year":"1988"},{"key":"b18","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910460106"},{"key":"b19","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1172\/JCI114675","volume":"86","author":"Maxwell M","year":"1990","journal-title":"J Clin Invest"},{"key":"b20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114820"},{"key":"b21","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1002\/ijc.2910460504","volume":"46","author":"Wang JL","year":"1990","journal-title":"Int J Cancer"},{"key":"b22","first-page":"2172","volume":"47","author":"Weisman AS","year":"1987","journal-title":"Cancer Res"},{"key":"b23","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1073\/pnas.87.1.128","volume":"87","author":"Westermark B","year":"1990","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["Journal of Neurosurgery"],"original-title":[],"link":[{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/81\/3\/article-p388.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/81\/3\/article-p388.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/thejns.org\/downloadpdf\/journals\/j-neurosurg\/81\/3\/article-p388.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,14]],"date-time":"2022-05-14T05:00:23Z","timestamp":1652504423000},"score":1,"resource":{"primary":{"URL":"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/81\/3\/article-p388.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9]]},"references-count":23,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.3171\/jns.1994.81.3.0388","relation":{},"ISSN":["0022-3085"],"issn-type":[{"value":"0022-3085","type":"print"}],"subject":[],"published":{"date-parts":[[1994,9]]}}}